Functional evaluation for effective compositions in seed oil of Korean pine

Abstract

The effect of seed oil of Korean pine (Pinus koraiensis) on the rats’ blood-fat and its anti-ageing function was studied for appraising the efficacy of the seed oil of Korean pine. Sixty experimental rats were randomly divided into 5 groups (half males and half females in each group) as normal control group, high fat diet control group, and three groups (Group 1 Group 2, Group 3) that were fed with feedstuff with the contents of the seed oil of 2.0g/(kg·d⁻¹), 4.0g/(kg·d⁻¹) and 8.0g/(kg·d⁻¹), respectively. The indexes such as superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and anti-oxidation capacity (AOC) were measured by Reagent Kit method. It was found that the seed oil of the Korean pine could reduce the content of triglyceride and improve SOD as well as GSH-PX activity in serum. These indexes of the rats in Group 2 fed with Korean pine seed oil of 4.0 g/(kg·d⁻¹) reached the significant level and those of rats in Group 3 fed with the seed oil of 8.0 g/(kg·d⁻¹) reached the extremely significant level. The results indicated the seed oil of Korean pine had function of regulating the level of blood-fat and anti-ageing.