Identification ofTaxus cuspidata Sieb. et Zucc endophytic fungi— new recorded-genus-species of China and the metabolite

Abstract

A total of 94 isolates of endophytic fungi were isolated from the bark of 200-yr.-oldTaxus cuspidata Sieb. et Zucc. in the primeval forest of the Changbai Mountain Natural Reserve, and 19 species of endophytic fungi were identified. Among the taxa 2 new species and 1 new varied species as well as the 6 known species of China had been described in a previous article published on Journal of Forestry Research, Vol. 14 (4): 290–294 (2003). This paper made a detail description on the morphological characteristics of the 9 new recorded-genus-species and 1 new record species with illustrations, includingStaphylotrichum coccosporum Meyer et Nicot,Botryodema lateritium Papendoorf & Upadhyay,Oedocephalum glomerulosum (Rulliard) Sacc.,Trichosporonoides oedocephalis Haskins & Spencer,Beniowskia sphaeroidea (Kal Chbrenner et Caeke) Mason,Thermomyces verrucosus Pugh, Blakeman & Morgan-jones,Gilmaniella humicola Brron,Arthrinum sphaerosperma (Per.:Fr.) Ellis,Ambrosiella brunnea (Verrall) Batra, andCytosporina notha Diedz (1 new record species). And the habitats, hosts, distributions and fruiting periods were recorded for the new recorded-genus-species. The determination result of thin layer chromatography (TLC) showed that the liquid fermentation metabolites of the strains could not react positively with taxoids developer— the vanillic aldehyde and dilute bismuth potassium iodide.

Keywords

Taxus / Endophytic fungi / Identification / New recorded-genus-species of China / S792.49 / S432.44 / A / 1007-662X(2004)01-0061-06

References

[1] Ainsworth G.C., Sparrow F.K., Sussman A.S. The Fuyi An advanced tretise 4A [M], 1973 Harbin and London: Academic Press 1-521.

[2] Barnett H.L., Barry B. Illustrated genera of imperfect fungi, 1972 3rd ed. Minneepolis Minnesota: Burgess Pub. Com. 1-241.

[3] Barron G.L. A new genus of theHyphomycetes from soll [J]. Mycologia, 1964, 56: 514-518.

[4] Barron, G.L. 1968. The genera ofHyphomyoetes from soil. [M]. Maryland: Baltimore, p1–364.

[5] Batra L.R. Ambrosia fungi: a Taxonomic Revision, and nutritional studies of some species [J]. Mycologia, 1967, 59: 976-1017.

[6] Diedicke H. Kryptogamenflora der Mark Brandenburg. IX, [M], 1912 Leipzig: Borntraeger Bros 1-545.

[7] Ellis M.B. DematiccousHyphomycete, VI, [J]. Mycol Papers C.M.I., 1965, 103: 1-46.

[8] Ellis M.B. DermatiaceousHyphomycetes, [M], 1971 England.: C.M.I, Kew, Surry 1-608.

[9] Ellis M.B., Ellis E.A., Ellis J.P. British marsh and fen fingi II [J]. Trans. Brit. Mycol. Soc., 1951, 34: 497-514.

[10] Zhongda Fang The study methods of plant disease [M], 1979 Beijing: Agriculture Publishing Company 102 160 (in Chinese)

[11] Grave W.B. British stem and leaf fungi (Coelonuycetes) [M], 1935 London and New York: Cambridge University Press 451-452.

[12] Haskins R.H., Spencer J.F.T. Trichosporonoides oedocophalis nov.gen., nov. sp. morphology, development, and taxonomic position [J]. Can. J. Bot., 1967, 45: 515-520.

[13] Mason E.W. List of fungi [J]. Mycol, Paers C.M.I., 1927, 2: 26-27.

[14] Ncot J, Meyer J UnHyphomycete nouveau Des sols tropicaux:Staphlotriclum coccosporum Meyer et Nicot [J]. Ball. Soc. Mycol. Fr., 1957, 72: 318-323.

[15] Papendorf M.C., Upadhyay H.P. Botryodena lateritium andB. rosratum gen. et spp. nov. From soil in south Africa and bragil [J]. Trans. Br. Mycol. Soc., 1969, 52(2): 257-265.

[16] Pugh G.J.F., Blakeman J.P., Morgan-jones G Thermonyces verrucosus sp. nov. andT. Lanuginosas [J]. Trans. Brit. Mycol. Soc., 1964, 47(1): 115-121.

[17] Saccardo P.A. Sylloge fuugorum [M]. Italy: Pavia, 1884, 3: 1-860.

[18] Saccardo P.A. Sylloge fuugorum [M]. Italy: Pavia, 1886, 4: 1-807.

[19] Sutton B.C. TheCoelomycetes [J], 1980 England: C.M.I. 1-696.

[20] Tsiklinsky P. Sur les mucedinies thermophilies [J]. Ann. Inst. Pasteur, 1899, 13: 500-505.

[21] Von Arx The genera of fungi sporulating in pure culture [M], 1974 Lehre: Veriag von Cramer 1-315.

[22] Yong Xiang, Jingxia Cui, Anguo Lu Isolation and screen of Taxus cuspidata Sieb. et Zucc. endophytic fungi [J]. Journal of Northeast Forestry University, 2002, 30(2): 30-34. (in Chinese)

[23] Yong Xiang, Jun Liu, Anguo Lu et al. Separation purification and structure identification of taxoids-produced endophytic fungi Alternaria alternata var.taxi 1011 [J]. Journal of Forestry Research, 2003, 14(3): 217-220.