Construction and analysis of a substracted cDNA library ofBetula platyphylla female inflorescence

Abstract

Female inflorescence ofBetula platyphylla was sampled at an interval of each two days to analyze the background of gene expression in floral phase. On the basis of SMART strategy, the driver cDNA was obtained from total RNA of the last sample and the tester cDNA was from that of the others by RT-PCR which were subsequently used to construct a subtracted cDNA library. The result of the ESTs (expression sequence tags) blastX showed that the genes in the subtracted cDNA library could be mainly clustered into 5 groups related to metabolism, transportation and signal transduction, cell cycle, stress response, and regulation. The relationship between gene expression and development was also discussed.

Keywords

Betula platyphylla / Subtracted cDNA library / SMART / S792.153 / A

References

[1] Ausubel F., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. Short protocols in molecular biology [M] (Chinese version in 1998), 1995 Beijing: Science Press 126-127.

[2] Cardon G.H., Hohmann S., Nettesheim K., Saedler H., Huijser P. Functional analysis of the Arabidopsis thaliana SBP-box gene SPL3: a novel gene involved in the floral transition [J]. Plant J., 1997, 12(2): 367-377.

[3] Cheng Y., Kato N., Wang W., Li J., Chen X. Two RNA binding proteins, HEN4 and HUA1, act in the processing of AGAMOUS pre-mRNA inArabidopsis thaliana [J]. Dev. Cell, 2003, 4(1): 53-66.

[4] Colombo L., Franken J., Koetje E., van Went J., Dons H.J., Angenent G.C., van Tunen A.J. The petunia MADS box gene FBP11 determines ovule identity [J]. Plant Cell, 1995, 7(11): 1859-1868.

[5] Cone E.S., Meyerowitz E.M. The war of the whorls: Genetics interactions controlling flower development [J]. Nature, 1991, 353: 31-37.

[6] Elo A., Lemmetyinen J., Turunen M.L., Tikka L., Sopanen T. Three MADS-box genes similar to APETALA1 and FRUITFULL from silver birch (Betula pendula) [J]. Physiol Plant, 2001, 112(1): 95-103.

[7] Felton G.W., Donato K.K., Del Vecchio R.J., Duffey S.S. Activation of foliar oxidases by insect feeding reduces nutritive quality of dietary protein of foliage for noctuid herbivores [J]. J Chem Ecol, 1989, 15: 2667-2693.

[8] Galan J.M., Peter M. Ubiquitin-dependent degradation of multiple F-box proteins by an autocatalytic mechanism [J]. Proc. Natl. Acad. Sci. USA, 1999, 96(16): 9124-9133.

[9] Golovkin M., Reddy A.S. A calmodulin-binding protein from Arabidopsis has an essential role in pollen germination [J]. Proc. Natl. Acad. Sci. USA, 2003, 100(18): 10558-10563.

[10] Jarvinen P., Lemmetyinen J., Savolainen O., Sopanen T. DNA sequence variation in BpMADS2 gene in two populations ofBetula pendula [J]. Mol. Ecol., 2003, 12(2): 369-384.

[11] Koning A.J., Tanimoto E.Y., Kiehne K., Rost T., Comai L. Cell-specific expression of plant histone H2A genes [J]. Plant Cell, 1991, 3(7): 657-665.

[12] Lannenpaa M., Janonen I., Holtta-Vuori M., Gardemeister M., Porali I., Sopanen T. A new SBP-box gene BpSPL1 in silver birch (Betula pendula) [J]. Physiol. Plant, 2004, 120(3): 491-500.

[13] Lauber M.H., Waizenegger I., Steinmann T., Schwarz H., Mayer U., Hwang I., Lukowitz W., Jurgens G. The Arabidopsis KNOLLE protein is a cytokinesis-specific syntaxin [J]. J Cell Biol., 1997, 139(6): 1485-1493.

[14] Lemmetyinen J., Hassinen M., Elo A., Porali I., Keinonen K., Makela H., Sopanen T. Functional characterization of SEPALLATA3 and AGAMOUS orthologues in silver birch [J]. Physiol Plant, 2004, 121(1): 149-162.

[15] Liu G., Sanchez-Fernandez R., Li Z.S., Rea P.A. Enhanced multispecificity of arabidopsis vacuolar multidrug resistance-associated protein-type ATP-binding cassette transporter, AtMRP2 [J]. J Biol Chem., 2001, 276(12): 8648-8656.

[16] Lu Y.P., Li Z.S., Drozdowicz Y.M., Hortensteiner S., Martinoia E., Rea P.A. AtMRP2, an Arabidopsis ATP binding cassette transporter able to transport glutathioneS-conjugates and chlorophyll catabolites: functional comparisons with Atmrp1 [J]. Plant Cell, 1998, 10(2): 267-282.

[17] McKinney E.C., Kandasamy M.K., Meagher R.B. Arabidopsis contains ancient classes of differentially expressed actin-related protein genes [J]. Plant Physiol., 2002, 128(3): 997-1007.

[18] Mizukami Y., Ma H. Determination of Arabidopsis floral meristem identity by AGAMOUS [J]. Plant Cell, 1997, 9(3): 393-408.

[19] Ng M., Yanofsky M.F. Activation of theArabidopsis B class homeotic genes by APETALA1 [J]. Plant Cell, 2001, 13(4): 739-753.

[20] Pelaz S., Ditta G.S., Baumann E., Wisman E., Yanofsky M.F. B and C floral organ identity functions require SEPALLATA MADS-box genes [J]. Nature, 2000, 405(6783): 200-203.

[21] Pelttari J., Hoja M.R., Yuan L., Liu J.G., Brundell E., Moens P., Santucci-Darmanin S., Jessberger R., Barbero J.L., Heyting C., Hoog C. A meiotic chromosomal core consisting of cohesin complex proteins recruits DNA recombination proteins and promotes synapsis in the absence of an axial element in mammalian meiotic cells [J]. Mol. Cell Biol., 2001, 21(16): 5667-5677.

[22] Sirko A., Brodzik R. Plant ureases: roles and regulation [J]. Acta Biochim Pol., 2000, 47(4): 1189-1195.

[23] Xiumin Su, Fengmao Wu, Tao Hong, Yueliang Wu, Chengjun Shi, Liming Wang Technology of anthesis inducing ofBetula platyphylla [J]. Journal of Liaoning Forestry Science & Technology, 2000, 3: 47-47. (in Chinese)

[24] Xu H., Swoboda I., Bhalla P.L., Singh M.B. Male gametic cell-specific, expression of H2A and H3 histone genes [J]. Plant Mol. Biol., 1999, 39(3): 607-614.

[25] Yanagisawa S. The Dof family of plant transcription factors [J]. Trends Plant Sci., 2002, 7(12): 555-560.