Dynamic feature of flavonoids content in different organs of larch (Larix gmelinii)

Abstract

Flavonoids in plants is very important in its ecological role and economic value. The dynamic features of flavonoids content in different organs of larch (Larix gmelinii) at different light and temperature conditions were investigated in this study. Results showed that the order of flavonoids content in different organs from high to low was 7.78% (stem bark)>2.79% (leaves) >1.72% (branches) >1.19% (stem xylem)and different organs had a great seasonal variation in flavonoids content, but the change of flavonoids content at different temperature was not obvious in different organs., The content of flavonoids in barck had, a positive correlation with temperature (R ²=0.75), but that in other organs had slight variation with the change of temperature. For all the tested organs, the flavonoids content in summer and autumn was approximately 3–4 times higher than in spring and winter. This is attributed to the great stress from environmental physical variables such as UV radiation, high temperature that induce the accumulation of flavonoids. The flavonoid content of sun leaves was evidently higher than that of shade leaves, and leaves at upper part of canopy had a higher flavonoids content compared with that at other parts. This result indicates that sun radiation could improve flavonoids production in leaves (R ²=0.76). The flavonoids may actively evolve in plant defenses to environmental stress, protecting larch from the damage of high temperature and radiation, and its main function is different in different organs.

Keywords

Larix gmelinii / Flavonoids / Dynamic features / Environmental stress / S791.22 / Q945.78 / A

References

[1] Bharti A.K., Khurana J.P. Mutants of Arabidopsis as tools to understand the regulation of phenylpropanoid pathway and UV-B protection mechanisms [J]. Photochem. Photobiol., 1997, 65: 765-776.

[2] Cen Y.P., Bornman J.F. The effect of exposure to enhanced UV-B radiation on the penetration of monochromatic and polychromatic UV-B radiation in leaves of Brassica napus [J]. Physiol. Plant, 1993, 87: 249-255.

[3] Chapell J., Hathlbrock K. Transcription of plant defence genes in response to UV light or fungal elicitor [J]. Nature, 1984, 311: 76-78.

[4] Cooper-Driver G.A., Bhattacharya M. Role of phenolics in plant evolution [J]. Phytochemistry, 1998, 49: 1165-1174.

[5] Day T.A. Relating UV-B radiation screening effectiveness of foliage to absorbing-compound concentration and anatomical characteristicsin a diverse group of plants [J]. Oecologia, 1993, 95: 542-550.

[6] Day T.A., Vogelmann T.C., DeLucia E.H. Are some plant life forms more effective than others in screening out ultraviolet-B radiation? [J]. Oecologia, 1992, 92: 513-519.

[7] Dixon R.A., Howles P.A., Lamb C. et al. Prospects for the metabolic engineering of bioactive flavonoids and related phenylpropanoid compounds [J]. Adv. Exp. Med. Biol., 1998, 439: 55-66.

[8] Dudt J.F., Shure D.J. The influence of light and nutrients on foliar phenolics and insect herbivory [J]. Ecology, 1994, 75: 86-98.

[9] Ellsworth D.S., Reich P.B. Canopy structure and vertical patterns of photosynthesis and related leaf traits in a deciduous forest [J]. Oecologia, 1993, 96: 169-178.

[10] Graham T.L. Flavonoid and isoflavonoid distribution in developing soybean seedling tissue and in seed and root exudates [J]. Plant Physiol., 1991, 95: 594-603.

[11] Harborne J.B., Mabry T.J. The flavonoids, advances in research [M], 1980 London: Chapman and Hall 155-167.

[12] Hideg E., Vass I. UV-B induced free radical production in plant leaves and isolated thylakoid membranes [J]. Plant Science, 1996, 115: 251-260.

[13] Hollinger D.Y. Canopy organization and foliage photosynthetic capacity in a broad-leaved evergreen montane forest [J]. Funct. Ecol., 1989, 3: 53-62.

[14] Krol M., Gray G.R., Hurry V.M. et al. Low-temperature stress and photoperiod affect an increased tolerance to photoinhibition in Pinus banksiana seedlings [J]. Can. J. Bot., 1995, 73: 1119-1127.

[15] Linda C.S., James D. S. Elevated ultraviolet-B radiation induces cross-protection to cold in leaves of rhododendron under field conditions [J]. Photochemistry and Photobiology, 2004, 79(2): 199-204.

[16] Lowman M.D. Temporal and spatial variability in insect grazing of the canopies of five Australian rainforest trees [J]. Aust. J. Ecol., 1985, 10: 7-24.

[17] McClure J.W. Physiology and function of flavonoids [C] The Flavonoids, 1975 Harbin: New York Academic Press 45-77.

[18] Panagopoulos I., Bornman J. F., Bjorn L.O. Response of sugar beet plants to ultraviolet-B (280–320 nm) radiation and Cercospora leaf spot disease [J]. Physiol. Plant, 1992, 84: 140-145.

[19] Rivero R.M., Juan M., Ruiz P.C. et al. Resistance to cold and heat stress: accumulation of phenolic compounds in tomato and watermelon plants [J]. Plant Science, 2001, 160: 315-321.

[20] Vog T., Giilz P.G. Isocratic column liquid chromatographic separation of a complex mixture of epicuticular flavonoid aglycones and intracellelar flavonol glycosides from Cistus laurifolius L [J]. J. Chromatogram, 1991, 537: 453-459.

[21] Waterman P.G., Mole S. Extrinsic factors influencing the production of secondary metabolites in plants [C] Insect-plant interactions, 1989 Florida: CRC Press 107-134.

[22] Wellmann E. UV radiation in photomorphogenesis [C] Encyclopedia of Plant Physiology, 1983 Berlin: Northeast Forestry University and Ecological Society of China, Press 745-756.

[23] Wollenweber E. Flavones and flavonols [C] The flavonoids advances in research since 1986, 1993 London: Chapman and Hall 537-545.

[24] Zaprometov M.N. Photoregulation of secondary metabolism in plants [J]. Sov. Plant Physiol., 1988, 34: 561-571.

[25] Ziska L.H., Teramura A.H., Sullivan J.H. et al. Influence of ultraviolet- B (UV-B) radiation on photosynthetic and growth characteristics in field-grown cassava (Manihot esculentum Crantz) [J]. Plant Cell Environment, 1993, 16: 73-79.

[26] Wenjie Wang, Fuchen Shi, Yuangang Zu et al. A trail to accelerate afforestation of Korean pine forests using a strip-cutting method for deciduous broad-leaves secondary forests in Northeast China and ecophysiological approach [J]. Eurasian J. Forest Research, 2001, 3: 27-48.

[27] Yongqin Wen, Lifen Xu, Zongyu Chen et al. Relationship between climatic factors and contents of petroleum ether extracts and polyphenol compounds in Yunnan tobacco [J]. Journal of Hunan Agricultural University (Natural Science), 2002, 28(2): 103-105. (in Chinese)

[28] Yuqing Wu, Yuyuan Guo Determination of tannin in cotton plant [J]. Chinese Journal of applied ecology, 2000, 11(2): 243-245. (in Chinese)