Determination of contents of 10-Hydroxycamptothecin inCamptotheca acuminata by high-performance liquid chromatogram

Abstract

The determination method of 10-hydroxycamptothecin inCamptotheca acuminatu fruits by high-performance liquid chromatogram (HPLC) was studied. The HPLC analysis was performed on a HIQ sil C₁₈(4.6×250 mm) column with mobile phase of acetonitrile-water (3:7, V:V), flow rate 1 mL·min⁻¹ and UV detective wavelength 266 nm. Extracting 10-hydroxycamptothecin by ultrasonic method from fruits ofC. acuminata to prepare samples for analysis was systematically discussed. The optimal extraction condition was carried out by 60% alcohol solution at 60°C for 50 minutes.

Keywords

10-hydroxycamptothecin / Camptotheca acuminata / HPLC / ultrasonic extraction method / O652.63 / B

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