Shoot tissue culture ofRobinia pseudoacacia f.decaisneana

Abstract

Robinia pseudoacacia f.decaisneana is a transfiguration ofRobinia pseudoacacia. For enhancing propagation co-efficient of the species, the experiment of shoot tissue culture ofRobinia pseudoacacia f. decaisneana was conducted in Forestry College of Shenyang Agricultural University from July 1999 to July 2001. The experiment included medium selection of explant induction survival, initial culture, subculture as well as rooting culture, and forming seedling with callus. The results showed that shoot segmentin vitro survive rate is larger in spring than in autumn, and green dense callus could form plantiet. The best medium for initial culture was SH+0.5mg/L BA+0.05mg/L NAA, with a propagation coefficient of 4.1 (per micro-cutting in a month), and for subculture it was B₅+0.5mg/L BA+0.05mg/L NAA+10mg/L Glu., with a propagation coefficient of 4.7. The best rooting medium was 1/2MS+0.5mg/L NAA+10mg/L Glu., with a rooting rate of 84.4%. These results provide reference data for reproduction of superior individuals ofRobinia pseudoacacia f. decaisneana.

Keywords

Robinia pseudoacacia f. decaisneana / Shoot segment / Tissue culture / Medium / S792.270.5 / S722.3 / A

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