Assessment of factors affectingin vitro shoot regeneration from axillary bud explant ofCamptotheca acuminata

Abstract

Axillary buds from 3-yr.-old seedlings ofCamptotheca acuminata in the greenhouse were cultured on the different basal media with different concentrations of growth regulators for shoot regeneration for studying the effects of different basal media, different concentrations of growth regulators (BA or TDZ), sucrose, agar and pH value on shoot regeneration from axillary bud. The results showed that B5 and WPM media were the optimal basal media and the optimal phyotohormone was BA of 1.0 mg/L or TDZ of 0.1 mg/L; The concentrations of sucrose of 30g/L and agar of 6g/L were most suitable for the shoot regeneration; pH value from 5.8 to 6.6 were broadly effective, but the best at pH 5.8.

Keywords

Camptotheca acuminata / Axillary bud / Shoot regeneration / Growth regulators / Basal media / S722.37 / A

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