Embryo culture and rapid propagation ofSyringa

Abstract

Embryo of lilacs (Syringa L) culture in vitro and the rapid propagation were studied. The orthogonal experiments, including the selection of basal medium, embryo age and other factors such as sugar, benzyladenine (BA), naphthalene acetic acid (NAA) and glutamine (Gln), were carried out. The results indicated that the optimal medium for embryo culture was Monnier medium supplemented with NAA (0.001 mg·L⁻¹), BA (0.1 mg·L⁻¹), sugar (50 g·L⁻¹), and Gln (400 mg·L⁻¹), with a germination rate of 91.7% at least; the optimal embryo age was 50 d; and Gln had significant effects on the germination rate of embryo. Moreover, the optimal medium for subculture was MS+BA (2 mg·L⁻¹)+NAA (0.001 mg·L⁻¹)+Gln(0.5 mg·L⁻¹), with the propagation coefficient of 3.6 at least.

Keywords

Syringa / Embryo-culture / Rapid propagation / S722.37 / A

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