Impact of anthocyanin fromMalva sylvestris on plasma lipids and free radical

Abstract

The effects of anthocyanin fromMalva sylvestris on plasma lipids and free radical were investigated by Reagent Kit method and in vitro assay. High fat model was set up with albino rats that were fed with different dosages of anthocyanin fromMalva sylvestris (0.03 g·d⁻¹, 0.04 g·d⁻¹ and 0.05 g·d⁻¹). The results showed that the total cholesterol was decreased by 19.7% at an anthocyanin of 0.04 g·d⁻¹ and triglyceride was decreased by 34.4% at an anthocyanin of 0.05 g·d⁻¹. In vitro assay, some indexes of anthocyanin were measured including the capability of scavenging free radical, reducing force and the capability of anti-lipid peroxidation by orthophenanthroline Fe⁺² oxidation-reduction method. The results indicated that the clearance rate of free radical reached to 43.46% when the content of anthocyanin was 0.20 mg·mL⁻¹ and the inhibition ratio of lipid peroxidation reached 18.82% when the content was 0.5 mg·mL⁻¹. Therefore anthocyanin is a kind of natural and effective free radical remover and antioxidant and can prevent from the formation of the thrombus and reduced the emergence of the cardiovascular disease.

Keywords

Anthocyanin / Plasma lipids / Free radical / Q493 / A

References

[1] Zhongxiang Fang, Yuanying Ni Research advances of anthocyanidin's physiological functions [J]. Guangzhou Food Science and Technology, 2001, 17(3): 60-62. (in Chinese)

[2] Mazza G. Recent Development in the Stabilization of Anthocyanins in Food Products [J]. Food Chemistry, 1987, 88(3): 207-225.

[3] Ghiselli A., Nardini M., Baldi A., Scaccini C. et al. Antioxidant activity of different phenolic fractions separated from an Italian red wine [J]. J. Agre. Food Chem., 1998, 46(3): 361-367.

[4] Hongying He, Huajun Li, Jian Yang et al. The progress on the research of functional foods of regulating blood fat [J]. Food and Mechanism, 2001, 81(1): 4-5. (in Chinese)

[5] Igarashi K., Kimura Y., Takenaka A. et al. Preventive Effects of Dietary Cabbage Acylated Ant-Hocyanins on Paraqual-Induced Oxidative Stress in Rats [J]. Biosci. Biotechnol. Biochem., 2000, 64(4): 1600-1607.

[6] Kayamori F., Igarashi K. Effects of dietary nasunio on the serum cholesterol level in rats [J]. Biosci. Biotechnol. Biochem., 1994, 58(4): 507-571.

[7] Dan Li, Gang Xiao, Xiaolin Ding et al. Study on the free radical scavenging effects of tartary buckwheat flavone [J]. Food Science and Technology, 2000, 19(6): 61-64. (in Chinese)

[8] Meunier M.T., Villie F.F., Villie F., Jonader M. et al. Antioxidant Activity of Different Phenolic Fractions Separated from An Italian Red Wine [J]. PlantaMed, 1987, 53(1): 12-15.

[9] Ross, R. 1993. Nature Reviews Drug Discovery [M].Nature Publishing Group, p362, 801.

[10] Tsudn T. Antioxidntive activity of anthoocyanin pigments cyanidin 3-0-β-D-gluceside and cyandin [J]. J. Agre. Food Chem., 1994, 42(4): 2407-2410.

[11] Guifu Yang The situation of security and regulation of pigment additives supplied in food, medicine and cosmetic exempted from inspection in USA [J]. China Food Additives, 1997, 26(3): 57-68. (in Chinese)

[12] Ying Yu, Wu Su, Dongzhi Wei et al. The research of free radicals externally-scavenging effects of proanthocyanidin [J]. Journal of East China of Science and Technology, 2002, 28(3): 318-320. (in Chinese)

[13] Xiaoling Zeng A study of Scavenging action of purslane aquenous extracts on oxygen free radical [J]. Bull. of Hunan Med. Univ., 1999, 24(2): 133-135. (in Chinese)

[14] Hongjie Zhang Study on Sports And Lipoprotein Metabolism [J]. Journal of Chengdu Physical Education Institute, 2004, 30(3): 75-77. (in Chinese)

[15] Jun Zhang, Weizhi Ji, Xiao-yun Chen et al. Preparation of oligomeric proanthocyanidins by adsorption chromatography [J]. Natural Product Research and Development, 2002, 14(4): 31-33. (in Chinese)

[16] Guangyao Zheng Physiological activity, pharmaceutical activity and application advances of proanthocyanidins extracted from grape seeds [J]. Journal of Chemical Industry of Forest Products, 2000, 34(1): 28-33. (in Chinese)