Genetic variation and division ofPinus sylvestris provenances by ISSR markers
Li Hui-yu , Jiang Jing , Liu Gui-feng , Ma Xu-jun , Dong Jing-xiang , Lin Shi-jie
Journal of Forestry Research ›› 2005, Vol. 16 ›› Issue (3) : 216 -218.
Genetic variation and division ofPinus sylvestris provenances by ISSR markers
Inter Simple Sequence Repeat (ISSR) was used to detect genetic variation among nine provenances, including 135 individuals. A total of 108 loci were amplified using 10 random primers. The differentiation of the percentage of polymorphic bands (PPB) among different provenances was evident, ranging from 27% to 54%, of which Honghuaerji provenance had the highest PPB and Kalunshan provenance had the lowest PPB. Shannon's Information index (I) at species level was 0.1581 and Nei's gene diversity (h) was 0.2393. Coefficient of gene differentiation (GST) calculated by Popgene was 0.3965, these results indicated that majority of genetic variation (60.35%) was found within provenances. According to dendrogram amongpinus sylvestriv provenances, nine provenances were divided into two provenance areas, namely Daxing's and Xiaoxing'an Mountains provenance area and Hulunbeier provenance area.
ISSR / Pinus sylvestris L.var mongolica litv / Provenance / Genetic variation / Division of provenances / S791.253 / A
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