Analysis of genetic relationship in 12 species ofSection Strobus with ISSR markers

Abstract

Genetic relationship of 12 species ofSection Strobus was analyzed with ISSR markers. 117 loci were detected with 12 ISSR primers. Percentage of polymorphic bands (PPB) varied from 5.93% to 19.92%.P. pumila had the highest levels of genetic differentiation andP. flexilis had lowest. Total genetic diversity (H_T) of 12 species inSection Strobus was 26.21%, of which intraspecific genetic diversity (Hs) was 7.66%, and interspecific genetic diversity (D_ST) was 18.55%, and the genetic variation in interspecies accounted for 70.78% of the total genetic diversity. According to the cluster results of genetic distance, the 12 species were classified into two groups. The first group includedP. griffithii, P. armandi, P. fenzeliana, P. kwangtungensis, P. strobus, P. monticola andP. wangii. The second group includedP. albicaulis, P. pumila, P. flexilis, P. sibirica andP. koraiensis.

Keywords

Pinus / ISSR-PCR / Genetic relationship / Q78 / A

References

[1] Yaolin Gui et al. Comparative dissection observation ofPinus needle in China [J]. Acta Botanica Sinica, 1963, 11(1): 44-66. (in Chinese)

[2] Yanfang Lu, Guifeng Liu, Jing Jiang et al. The ISSR-PCR reaction system's establishment about seed's endosperm of five needles pine [J]. Bulletin of Botanical Research, 2003, 23(4): 429-432.

[3] Shaw, Russell George The genusPinus [M] Arnold Arbor. Publ., 1914, 5: 1-96.

[4] Gang Wu, Zhongwei Feng The sociological characteristics and biomass of stone pine forests in China [J]. Acta Ecological Sinica, 1995, 15(3): 260-267.

[5] Guangyi Zhao, Wenjie Liu, Bosheng Zhao Comparative morphological studies on the three kinds ofSubsect. Cembrae ofPinus from Daxing'an Mountains in China [J]. Journal of Northeast Forestry University, 1998, 26(3): 1-5. (in Chinese)

[6] Yuping Zou, Song Ge, Xiaodong Wang Molecular markers in systematic and evolutionary botany [M], 2001 Beijing: Science Press 167 167 (in Chinese)