Assembling a physical map of the genome by marker sequences

Abstract

Molecular genetic maps were commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs). Here we described methodology-marker sequences in a new mapping based on recent documents. With the methods they were unique sequences detected by the polymerase chain reaction (PCR). Each of the methods had its limitations and the current trend was to integrate the maps produced by the different methods. Market sequences contained mainly expressed sequence tags (ESTs), polymorphic sequence-tagged sites (STSs), randomly amplified polymorphic DNA (RAPDs), cleaved amplified polymorphic sequences (CAPS), amplified fragment length polymorphism (AFLPs), genome sequence sampling (GSS) and sequence-tagged connectors (STCs) in this paper.

Keywords

Marker sequences / Sequence-tagged sites / Expressed sequence tags / Randomly amplified polymorphic DNA / Cleaved amplified polymorphic sequences / Amplified fragment length polymorphism / Genome sequence sampling / Sequence-tagged connectors / Q75 / A