New cell line establishment of a cell line from embryos ofDendrolimus superans

Abstract

A new cell line was established from 5-day-old embryonated eggs ofDendrolimus superans and has been designated NEAU-Ds-950821. The cell line consists of mixture of cell types, including majority of spherical shaped cells and a few of spindle shaped cells. The cell line has a population of doubling time of 52.6 h. Chromosome analysis revealed typical lepidopteran chromosomes. Isozyme characterization of Esterase showed the patterns were different from other three cell lines (Ms-927311, Xc-920730, and SF-21AE). Virus infectivity tests revealed the cell line can supportD. superans cytoplasmic polyhedrosis virus.

Keywords

Dendrolimus superans / Embryonated eggs / Characterization / Cell lines

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