RAPD analysis on genomic DNA of male and female plants ofAcer negundo L

Zhao Linsen; Zhang Qiang; Ma Jinxia; Xu Jian; Zhang Hua

doi:10.1007/BF02855422

Journal of Forestry Research ›› 1999, Vol. 10 ›› Issue (3) : 152 -154. DOI: 10.1007/BF02855422

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RAPD analysis on genomic DNA of male and female plants ofAcer negundo L

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Abstract

Genomic DNAs were isolated from both male and female plants ofAcer negundo L., by modified CTAB method of Doyle and Doyle for plant genomic DNA isolation and consequently tested with RAPD technique. Some sex-related specific oxyribonucleic acid bands were amplified by a group of decamer oligonucleotide random primers. This indicated that some genetic marks related to sexes of the species were found, which laid a foundation for sapling sex identification ofAcer negundo L.

Keywords

Genomic DNA / RAPD / Sex identification

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Zhao Linsen, Zhang Qiang, Ma Jinxia, Xu Jian, Zhang Hua. RAPD analysis on genomic DNA of male and female plants ofAcer negundo L. Journal of Forestry Research, 1999, 10(3): 152-154 DOI:10.1007/BF02855422

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References

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[1]	Yuquan Chen Research Techniques of Biochemistry, 1995 Beijing: Publishing House of China Agriculture

[2]	Rongzhao Fu et al. Handbook of Plant Genetic Transformation Techniques, 1994 Beijing: China Science and Technology Publishing House

[3]	Hormaza J. I. et al. Identification of a RAPD marker linked to sex determination in Pistacia vera using bulked segregant analysis Theor. Appl. Genet., 1994, 89: 9-13.