Experiments on decolourization with 122 resin for extraction of gibberellins from solid medium under different conditions

Abstract

The extracting liquid of gibberellins (Gibberella fujikuroi) from solid medium for was decolorized separately with 75%, 95% alcohol, and distilled water in static adsorption and vibrating way for different durations. The results showed that the content of GA₃ in efflux extracted with alcohol is 10% higher than that with distilled water. With the increase of the durations of extraction, the content of GA₃ increases and the dissolution of pigments also increase. For extraction of GA₃ cultured in solid medium, the best decolorizing result was obtained when it was extracted with 75%-95% alcohol in static way for 1–3 h, or in a vibrating way for 30–60 min, and then to decolor in a static way for 30–60 min in 122 resin column.

Keywords

Gibberellins (GA₃) / Solid Culture / Extraction / Adsorption / Decolourization 122-Resin