Effect of priming on the germination ofPeltophorum dubium seeds under water stress

Abstract

Peltophorum dubium seeds provided by Anhembi, SP were scarified in 98% H₂SO₄ for 15 min to overcome mechanical dormancy. Seeds were primed in solutions of 0.2% Captan at 10 °C and 27°C, PEG 6000 −1.0 MPa at 10°C and 27°C, 0.5 mol KNO₃, 0.75 Mol KNO₃, 1.0 Mol KNO₃. Eight treatments including the primed seeds and nonprimed seeds, five replicates with 100 seeds for each treatment, were set to 15-cm-Petri dish with double filter paper moistened with testing solution PEG in refrigerator at 27°C. For the experiments of all the groups, osmotic potential were 0.0, −0.2, −0.4, −0.6, −0.8, −1.0, −1.2, and −1.4 MPa.P. dubium seeds were also set to water stress experiment in rolled paper with PEG solutions from 0.0 to −1.0 M Pa. Germination percentage decreased with the increase of PEG concentration. Control group had a better germination percentage than other groups. Germination hardly occurred in PEG −1.4 MPa.

Keywords

Priming / Osmotic potential / Germination / Seeds,Peltophorum dubium

References

[1] Atherton J.G., Farooque A.M. High temperature and germination in spinach: II. Effects of osmotic priming [J]. Scientia Hort., 1983, 19: 221-227.

[2] Bewley J.D., Black M. Seeds: Physiology of development and germination [M], 1986 Harbin and London: Plenum Press 231-233.

[3] Bradford K.J. Manipulation of seed water relations via osmotic priming improve germination under stress conditions. Proc. Seed germination Under Environ. Stress [J]. Hort Science., 1986, 2: 1105-1112.

[4] Cantliffe D.J. Priming of lettuce seed of early and uniform emergence under conditions of environmental stress [J]. Acta Hort., 1981, 122: 29-38.

[5] Carpenter W.J. Priming dusty miller seeds: Role of aeration, temperature, and relative humidity [J]. Hort Science, 1990, 25: 299-302.

[6] Cordeiro S.R., Di Stefano G.J.L. Efeito del estrés osmótico sobre la germinaçao de semillas deTecoma stans (Bignoniaceae.) [J]. Ver. Bras. Sem., 1991, 39: 107-110.

[7] Cordoba G.A.T. et al. Osmocondiçionamentos, secagem e armazenamento deEucalyptus citriodora. Hook eEucalyptus grandis W. Hill [J]. Rev. Bras. Sem., 1995, 17: 81-95.

[8] Haridi M.B. Effect of osmotic priming with polyethylene glycol on germination of Pinus elliottii seeds [J]. Seed Sci. & Technol., 1985, 13: 669-674.

[9] Khan A.A. Preplant physiological seeds conditioning Horticultural Review, 1992, 14: 131-181.

[10] Yoon B.Y., Lang H.J., Cobb B.G. Priming with salt solutions improves germination of pansy seed at high temperatures [J]. Hort Science, 1997, 32(2): 248-250.