Feasibility of skin carotenoids as a biomarker of vegetable intake among Mexican-Origin children in a community setting: A cross-sectional pilot study

Abstract

Background and purpose: Studies in adults and children have demonstrated a strong correlation of skin carotenoids with fruit and vegetable (F/V) intake. There are few studies testing the usefulness of this methodology in a community setting. This study aimed to determine the feasibility of resonance Raman spectroscopy (RRS) skin carotenoid intensity scores in young children to assess F/V intake.

Methods: A cross-sectional study design was used to capture the RRS skin carotenoid intensity scores using the Pharmanex BioPhotonic Scanner. The RRS skin carotenoid intensity score ranges from 0 to 70,000+ with increasing scores indicating a higher carotenoid concentration. Children’s dietary intake of F/V was assessed within one-month period using a 26-item food frequency questionnaire (FFQ) and multiple pass 24-hour dietary recalls (24HDRs) administered to the parent of the child. Participants were 51 Mexican-origin children (ages 3-8 years) living in two rural California school districts during July-August 2014. RRS skin carotenoid intensity scores; frequency of fruit and vegetable intake from FFQ; daily servings of fruit and vegetables and carotenoid intake from 24HDR. Spearman correlation was used to determine the correlation between RRS skin carotenoid intensity scores and average 24HDR F/V intake and FFQ F/V intake.

Results: The results of the study demonstrate a significant positive correlation between skin carotenoid levels and parent-reported 24HDR vegetable consumption (r =.41, p =.003) but not with 24HDR fruit, FFQ fruit, or FFQ vegetable consumption.

Conclusion: The use of skin carotenoid measurements may be more indicative of short-term vegetable consumption than overall fruit and vegetable or fruit-only consumption in this population of Mexican-origin children.

Keywords

Carotenoids / Children / Mexican / Fruits and vegetables / Resonance Raman spectroscopy

References

[1] Monsen ER, Van Horn L. Research: Successful Approaches: American Dietetic Associati; 2007.

[2] Livingstone MBE, Robson PJ. easurement of dietary intake in children. Proceedings of the Nutrition Society. 2000; 59(02): 279-93. https://doi.org/10.1017/S0029665100000318

[3] Burrows T, Truby H, Morgan P, et al. A comparison and validation of child versus parent reporting of children’s energy intake using food frequency questionnaires versus food records: who’s an accurate reporter? Clinical Nutrition. 2013; 32(4): 613-8. PMid:23206381 https://doi.org/10.1016/j.clnu.2012.11.006

[4] Burrows TL, Martin RJ, Collins CE. A Systematic Review of the Validity of Dietary Assessment Methods in Children when Compared with the Method of Doubly Labeled Water. Journal of the American Dietetic Association. 2010; 110(10): 1501-10. https://doi.org/10.1016/j.jada.2010.07.008

[5] Food Nutrition Board IoM. Dietary Reference Intakes for Vitamin C, Vitamin E, Selenium, and Carotenoids. National Academy of Sciences. 2000.

[6] Resnicow K, Odom E, Wang T, et al. Validation of Three Food Frequency Questionnaires and 24-Hour Recalls with Serum Carotenoid Levels in a Sample of African-American Adults. American Journal of Epidemiology. 2000; 152(11): 1072-80. https://doi.org/10.1093/aje/152.11.1072

[7] Burrows TL, Williams R, Rollo M, et al. Plasma carotenoid levels as biomarkers of dietary carotenoid consumption: A systematic review of the validation studies. Journal of Nutrition & Intermediary Metabolism. 2015; 2(1-2): 15-64. https://doi.org/10.1016/j.jnim.2015.05.001

[8] Pezdirc K, Hutchesson MJ, Williams RL, et al. Consuming High-Carotenoid Fruit and Vegetables Influences Skin Yellowness and Plasma Carotenoids in Young Women: A Single-Blind Randomized Crossover Trial. Journal of the Academy of Nutrition and Dietetics. 2016; 116(8): 1257-65. https://doi.org/10.1016/j.jand.2016.03.012

[9] Hammond BR, Wooten BR. Resonance Raman spectroscopic measurement of carotenoids in the skin and retina. Journal of Biomedical Optics. 2005; 10(5): 054002. https://doi.org/10.1117/1.2116767

[10] Mayne ST, Cartmel B, Scarmo S, et al. Noninvasive assessment of dermal carotenoids as a biomarker of fruit and vegetable intake. The American Journal of Clinical Nutrition. 2010; 92(4): 794-800. https://doi.org/10.3945/ajcn.2010.29707

[11] Jahns L, Johnson LK, Mayne ST, et al. Skin and plasma carotenoid response to a provided intervention diet high in vegetables and fruit: uptake and depletion kinetics. The American Journal of Clinical Nutrition. 2014; 100(3): 930-7. https://doi.org/10.3945/ajcn.114.086900

[12] Aguilar SS, Wengreen HJ, Dew J. Skin Carotenoid Response to a High-Carotenoid Juice in Children: A Randomized Clinical Trial. Journal of the Academy of Nutrition and Dietetics. 2015; 115(11): 1771-8. https://doi.org/10.1016/j.jand.2015.06.011

[13] Nguyen LM, Scherr RE, Linnell JD, et al. Evaluating the relationship between plasma and skin carotenoids and reported dietary intake in elementary school children to assess fruit and vegetable intake. Archives of Biochemistry and Biophysics. 2015. https://doi.org/10.1016/j.abb.2015.02.015

[14] de la Torre A, Sadeghi B, Green R, et al. Ninos Sanos, Familia Sana: Mexican immigrant study protocol for a multifaceted CBPR intervention to combat childhood obesity in two rural California towns. BMC Public Health. 2013; 13(1): 1033. https://doi.org/10.1186/1471-2458-13-1033

[15] Scarmo S, Cartmel B, Lin H, et al. Single v. multiple measures of skin carotenoids by resonance Raman spectroscopy as a biomarker of usual carotenoid status. British Journal of Nutrition. 2013; FirstView: 1-7. https://doi.org/10.1017/S000711451200582X

[16] Scarmo S, Henebery K, Peracchio H, et al. Skin carotenoid status measured by resonance Raman spectroscopy as a biomarker of fruit and vegetable intake in preschool children. European Journal of Clinical Nutrition. 2012; 66(5): 555-60. https://doi.org/10.1038/ejcn.2012.31

[17] Wengreen HJ, Madden GJ, Aguilar SS, et al. Incentivizing children’s fruit and vegetable consumption: Results of a United States pilot study of the Food Dudes program. Journal of Nutrition Education and Behavior. 2013; 45(1): 54-9. PMid:23178042 https://doi.org/10.1016/j.jneb.2012.06.001

[18] NDSR. 2012 ed. Minneapolis, MN: Nutrition Coordinating Center; 2012.

[19] U.S. Department of Agriculture U.S. Department of Health and Human Services. Dietary Guidelines for Americans, 2015. 8th ed2015.

[20] Kaiser LL, Aguilera AL, Horowitz M, et al. Correlates of food patterns in young Latino children at high risk of obesity. Public Health Nutr. 2015: 1-9. https://doi.org/10.1017/S1368980014003309

[21] Kaiser L, Martin A, Metz D, et al. Food insecurity prominent among low-income California Latinos. California Agriculture. 2004; 58(1): 18-23. https://doi.org/10.3733/ca.v058n01p18

[22] Ermakov IV, Gellermann W. Validation model for Raman based skin carotenoid detection. Archives of Biochemistry and Biophysics. 2010; 504(1): 40-9. https://doi.org/10.1016/j.abb.2010.07.023

[23] Lohan T, Roche A, Martorell R. Anthropometric Standardization Reference Manual: Champaign: Human Kinetics Publishers; 1988.

[24] Kuczmarski R, Ogden C, Grummer-Strawn L, et al. CDC growth charts: United States. Adv Data. 2000; 1-27. PMid:11183293

[25] SAS. 9.3 ed. Cary, NC, USA: SAS Institute Inc.; 2011.

[26] Nielsen SJ, Rossen LM, Harris DM, et al. Fruit and vegetable consumption of US youth, 2009-2010. NCHS data brief. 2014; 156: 1-8.

[27] Food Availability and Consumption Internet. United States Department of Agriculture. 2012. Available from: http://www.ers.usda.gov/data-products/ag-and-food-statistics-charting-the-essentials/food-availability-and-consumption.aspx#.U4-WDJRg6K4