Chemical ingredients in personal care products and their hormetic effects on freshwater photobacteria

Abstract

Personal care products (PCPs), including skin lotions, toners, and make-up waters, are ubiquitous in daily life. Prior research has demonstrated hormesis, a biphasic dose-response phenomenon, in these PCPs on the freshwater photobacterium Vibrio qinghaiensis sp. -Q67 (Q67). Given that PCPs contain various active chemical components, their hormetic effects are intricately linked to these ingredients. However, studies focusing on the concentration-response relationships of individual PCP chemical ingredients are scarce. Building upon our previous work, we analyzed the ingredients of 6 PCPs and employed Q67 as a model organism to investigate the concentration-response relationships of these ingredients. Our analysis of 6 representative PCPs revealed 49 chemical ingredients, categorized into 10 moisturizers, 5 preservatives, 5 emulsifiers, 5 emollients, 20 ingredients with known efficacy, and 4 with unknown efficacy. Among these 49 chemical ingredients, 30 water-soluble ones were suitable for toxicity testing. At 0.25 h, 23 chemical ingredients exhibited S-shaped concentration-response curves (CRCs) with EC₅₀ values spanning from 6.943E-5 g/mL [lactic acid (LAA)] to 1.032 g/mL [sorbitol (SBO)], while 2 [PEG-7 glyceryl cocoate (PGC) and Tween 80 (TW80)] showed hormesis with J-shaped CRCs, and 5 [adenosine (ADE), dexpanthenol (DEX), E4C, E6C, serine (SER)] lacked discernible CRCs. At 12 h, 13 chemical ingredients displayed S-shaped CRCs with EC₅₀ values ranging from 4.402E-5 g/mL (E6C) to 1.961E-1 g/mL [dicaprylyl carbonate (DIC)], while 16 showed J-shaped CRCs, with EC₅₀, zero effective concentration point (ZEP), EC_min, and E_min values indicating a consistent variation range spanning 4 orders of magnitude. These findings underscore the importance of considering individual chemical ingredients in assessing the risks associated with PCPs.

Keywords

Luminous bacteria / cosmetics / stimulatory effect / chronic toxicity

References

[1] Daughton CG.Pharmaceuticals and personal care products in the environment: agents of subtle change?.Environ Health Perspect1999;107:907-38 PMCID:PMC1566206

[2] Nicolopoulou-Stamati P,Sasco AJ.Cosmetics as endocrine disruptors: are they a health risk?.Rev Endocr Metab Disord2015;16:373-83

[3] Kessler R.More than cosmetic changes: taking stock of personal care product safety.Environ Health Perspect2015;123:A120-7 PMCID:PMC4421754

[4] Li J,Xia W.Variations, determinants, and coexposure patterns of personal care product chemicals among Chinese pregnant women: a longitudinal study.Environ Sci Technol2019;53:6546-55

[5] Fandiño-Del-Rio M,Calafat AM.Recent use of consumer and personal care products and exposures to select endocrine disrupting chemicals among urban children with asthma.J Expo Sci Environ Epidemiol2024;34:637-46

[6] Goldberg M,Stevens DR.Environmental phenol exposures in 6- to 12-week-old infants: the infant feeding and early development (IFED) study.Environ Res2024;252:119075 PMCID:PMC11178257

[7] Sdougkou K,Bonnefille B.Longitudinal exposomics in a multiomic wellness cohort reveals distinctive and dynamic environmental chemical mixtures in blood.Environ Sci Technol2024;58:16302-15 PMCID:PMC11411717

[8] García-Pimentel M,Castaño-Ortiz JM,León VM.Occurrence and distribution of contaminants of legacy and emerging concern in surface waters of two Western Mediterranean coastal areas: Mar Menor Lagoon and Ebro Delta.Mar Pollut Bull2023;187:114542

[9] Lu S,Wang B.Comprehensive profiling of the distribution, risks and priority of pharmaceuticals and personal care products: a large-scale study from rivers to coastal seas.Water Res2023;230:119591

[10] Ghosh R,Bhaduri S,Behera M.Occurrence, fate, transport, and removal technologies of emerging contaminants: a review on recent advances and future perspectives.CLEAN Soil Air Water2024;52:2300259

[11] K’oreje K,Van Langenhove H.Occurrence and point-of-use treatment of contaminants of emerging concern in groundwater of the Nzoia River basin, Kenya.Environ Pollut2022;297:118725

[12] Lu J,Li H,Yang Z.Occurrence of and human exposure to parabens, benzophenones, benzotriazoles, triclosan and triclocarban in outdoor swimming pool water in Changsha, China.Sci Total Environ2017;605-6:1064-9

[13] Vecchiato M,Spolaor A.Fragrances and PAHs in snow and seawater of Ny-Ålesund (Svalbard): local and long-range contamination.Environ Pollut2018;242:1740-7

[14] Montes-Grajales D,Miranda-Castro W.Occurrence of personal care products as emerging chemicals of concern in water resources: a review.Sci Total Environ2017;595:601-14

[15] Kek T,Virant-Klun I.Exposure to endocrine disrupting chemicals (bisphenols, parabens, and triclosan) and their associations with preterm birth in humans.Reprod Toxicol2024;125:108580

[16] Veettil P, Nikarthil Sidhick J, Kavungal Abdulkhader S, Ms SP, Kumari Chidambaran C. Triclosan, an antimicrobial drug, induced reproductive impairment in the freshwater fish, Anabas testudineus (Bloch, 1792).Toxicol Ind Health2024;40:254-71

[17] Wimmerova L,Spacilova M,Krejcikova S.Toxicity assessment and treatment options of diclofenac and triclosan dissolved in water.Toxics2022;10:422 PMCID:PMC9415529

[18] Fent K,Rapp M.Widespread occurrence of estrogenic UV-filters in aquatic ecosystems in Switzerland.Environ Pollut2010;158:1817-24

[19] Carstensen L,Börnick H.Structure-related endocrine-disrupting potential of environmental transformation products of benzophenone-type UV filters: a review.J Hazard Mater2022;430:128495

[20] Mao JF,Ong CN,Jong MC.Assessment of human exposure to benzophenone-type UV filters: a review.Environ Int2022;167:107405

[21] Ghazipura M,Arslan A.Exposure to benzophenone-3 and reproductive toxicity: a systematic review of human and animal studies.Reprod Toxicol2017;73:175-83

[22] Zheng X,Zhao L.Binding and activation of estrogen related receptor γ as possible molecular initiating events of hydroxylated benzophenones endocrine disruption toxicity.Environ Pollut2020;263:114656

[23] Zhu Q,Jia J.Occurrence, distribution, and human exposure of several endocrine-disrupting chemicals in indoor dust: a nationwide study.Environ Sci Technol2020;54:11333-43

[24] Wang W,Zhu Q.Occurrence of synthetic phenolic antioxidants in foodstuffs from ten provinces in China and its implications for human dietary exposure.Food Chem Toxicol2022;165:113134

[25] Tang S,Qiao X.Prenatal exposure to emerging plasticizers and synthetic antioxidants and their potency to cross human placenta.Environ Sci Technol2022;56:8507-17

[26] Xu YQ,Wang ZJ,Qu R.Commercial personal care product mixtures exhibit hormetic concentration-responses to Vibrio qinghaiensis sp.-Q67.Ecotoxicol Environ Saf2018;162:304-11

[27] Xu YQ,Wang ZJ,Liu SS.Transfer pattern of hormesis into personal care product mixtures from typical hormesis-inducing compounds.Sci Total Environ2023;855:158981

[28] Zhu XW,Ge HL.Comparison between the short-term and the long-term toxicity of six triazine herbicides on photobacteria Q67.Water Res2009;43:1731-9

[29] Liu S,Zhang Y.APTox: assessment and prediction on toxicity of chemical mixtures.Acta Chim Sinica2012;70:1511

[30] Wang ZJ,Qu R.JSFit: a method for the fitting and prediction of J- and S-shaped concentration-response curves.RSC Adv2018;8:6572-80 PMCID:PMC9078288

[31] Stroppel L,Cebulla M.Antimicrobial preservatives for protein and peptide formulations: an overview.Pharmaceutics2023;15:563 PMCID:PMC10217790

[32] Esimbekova EN,Kratasyuk VA.Alternative enzyme inhibition assay for safety evaluation of food preservatives.Life2023;13:1243 PMCID:PMC10300798

[33] Reeder MJ,Aravamuthan SR.More than just methylisothiazolinone: Retrospective analysis of patients with isothiazolinone allergy in North America, 2017-2020.J Am Acad Dermatol2024;90:319-27

[34] Molin EAD,Lazzari VM.A systematic review focused on lubricant use and sperm quality: improving human reproductive success by informing lubricants toxicity.J Appl Toxicol2024;44:1470-7

[35] Sala-Hamrick KE,Polemi KM,Colacino JA.High-throughput transcriptomics of nontumorigenic breast cells exposed to environmentally relevant chemicals.Environ Health Perspect2024;132:47002 PMCID:PMC10990114

[36] Jun T,Won YY.Engineered polymeric excipients for enhancing the stability of protein biologics: poly(N-isopropylacrylamide)-poly(ethylene glycol) (PNIPAM-PEG) block copolymers.Int J Pharm2024;664:124636

[37] Lou H,Kuczera K.Coarse-grained molecular dynamics simulation of heterogeneous polysorbate 80 surfactants and their interactions with small molecules and proteins.Mol Pharm2024;21:5041-52

[38] Friis UF,Thyssen JP.A patient’s drawing helped the physician to make the correct diagnosis: occupational contact allergy to isothiazolinone.Contact Dermatitis2012;67:174-6

[39] Tang L,Li J.Isothiazolinone disrupts reproductive endocrinology by targeting the G-protein-coupled receptor signaling.Environ Sci Technol2024;58:1076-87

[40] Silva V,Soares P,Borges F.Isothiazolinone biocides: chemistry, biological, and toxicity profiles.Molecules2020;25:991 PMCID:PMC7070760

[41] Schwensen JF,Bossi R.Methylisothiazolinone and benzisothiazolinone are widely used in paint: a multicentre study of paints from five European countries.Contact Dermatitis2015;72:127-38

[42] Amat AM,López-Pérez M,Palacios S.Effect of methylisothiazolinone on biological treatment: efficiency of SBRs and bioindicative studies.Environ Eng Sci2015;32:479-85

[43] Paijens C,Caupos E,Bressy A.Determination of 18 biocides in both the dissolved and particulate fractions of urban and surface waters by HPLC-MS/MS.Water Air Soil Pollut2020;231:4546

[44] Paun I,Iancu VI.Occurrence and transport of isothiazolinone-type biocides from commercial products to aquatic environment and environmental risk assessment.Int J Environ Res Public Health2022;19:7777 PMCID:PMC9266048

[45] Lee S.Toxicological signature for thyroid endocrine disruption of dichlorooctylisothiazolinone in zebrafish larvae.Ecotoxicology2023;32:38-45

[46] Lee S,Kho Y.Effects of methylisothiazolinone and octylisothiazolinone on development and thyroid endocrine system in zebrafish larvae.J Hazard Mater2022;425:127994

[47] Lanigan RS.Final report on the safety assessment of PEG-7, -30, -40, -78, and -80 glyceryl cocoate.Int J Toxicol1999;18:33-42Available from: https://www.semanticscholar.org/paper/Final. [Last accessed on 19 Dec 2024]

[48] Humann RA.Potential trypanocidal activity of glycerol analogues.ChemistryOpen2024;13:e202400094 PMCID:PMC11625932

[49] Yi L,Piao C.The protective effects of 1,2-propanediol against radiation-induced hematopoietic injury in mice.Biomed Pharmacother2019;114:108806

[50] Cheng R,Ding TT,Tao MT.Time-dependent hormesis transfer from five high-frequency personal care product components to mixtures.Environ Res2024;248:118418

[51] Ortiz de García S, García-Encina PA, Irusta-Mata R. Dose-response behavior of the bacterium Vibrio fischeri exposed to pharmaceuticals and personal care products.Ecotoxicology2016;25:141-62

[52] Zheng Q,Liu S.Hormesis of some organic solvents on Vibrio qinghaiensis sp.-Q67 from first binding to the β subunit of luciferase.RSC Adv2017;7:37636-42