Expression of PANDA, LincRNA-p21, PUMA in lung tissues of lung cancer patients in the Xuanwei and non-Xuanwei areas of Yunnan Province

Abstract

Aim: To study the expression of PANDA, LincRNA-p21, and PUMA in lung tissue of patients with lung cancer from Xuanwei of Yunnan Province. Methods: Forty-five cases of lung cancer patients from Xuanwei and 42 lung cancer cases from non-Xuanwei were enrolled. Extraction of RNA was done using the Trizol kit. Real-time fluorescence quantitative PCR assay was done to obtain the relative expression. Results: Expressions of PANDA, LincRNA-p21, and PUMA in male and female patients or in squamous cell carcinoma and adenocarcinoma were not significantly different (P > 0.05). However, expression of LincRNA-p21 in Xuanwei patients was higher than non-Xuanwei patients (P < 0.05). Expression of PUMA in tumor tissue was lower than that in normal lung tissue (P < 0.05), and in Xuanwei patients was lower than non-Xuanwei patients (P < 0.05). In patients from non-Xuanwei regions, expression of LincRNA-p21 in patients with smoking index > 400 was higher than in those < 400 and non-smokers. Conclusion: Expressions of PANDA, LincRNA-p21, and PUMA in lung tissues have no gender differences or tissue specificity. High expression of LincRNA-p21 in Xuanwei patients may have relationship with cell damage caused by coal burning pollution in Xuanwei.

Keywords

Lung cancer / PANDA / LincRNA-p21 / PUMA / Xuanwei area

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