Nutritional composition, antioxidant properties, and molecular docking strategy of muricidae operculum (Chicoreus ramosus)

Abstract

The murrcidae gastropod operculum has many therapeutic uses in ayurveda, including treating cancer, gastric, hepatic, cardiovascular, and immunological disorders. Antibacterial, cell reinforcement, FTIR, and mass spectrum datas were used to identify important functional groups and chemical constituents in Chicoreus ramosus operculum concentrate. At 100 mg/L, the operculum extract showed stronger inhibitory movement (125 mm) against Bacillus subtilis and less (08 mm) against Staphylococcus aureus. Operculum extract’s biochemical composition, total antioxidant properties, protein denaturation, metal chelation movement, all-out cell reinforcement action, and anti-diabetic action were 85.71%, 80.98%, 32.03%, and 76.47% at 1000 µg/mL concentration. The operculum remove FTIR showed nine significant groups, including amines, esters, and fragrant mixtures. 11 dynamic mixtures from GC–MS analysis of operculum rough concentrate. These bioactive fractions interacted with IL 23 in molecular docking experiments. Androst-1-en-3-one, Bis (2-ethylhexyl) phthalate, and 3-Methoxy-2,4,5-trifluorobenzoic acid had the highest docking scores and target protein receptor interactions. -11.9 kcal/mol, -08.6 kcal/mol and -7.7 kcal/mol are the maximum scores. These compounds are therapeutic and antimicrobial. These bioactive compounds in operculum extracts allow C. ramosus to be used in conventional medicine and may lead to the development of new drugs.

Keywords

Chicoreus ramosus / Operculum extract / Bioactive efficacy / Bioactive compounds / Interleukin-23 gene

References

[1] Barco A, Claremont M, Reid DG, et al. A molecular phylogenetic framework for the Muricidae a diverse family of carnivorous gastropods. Mol Phylogenet Evol. 2010; 56(3):1025–1039.

[2] Levis GE, Castilla JC. A review of the world marine gastropod fishery: evolution of catches, management and the Chilean experience. Rev Fish.Biol. 2001;11:283–300.

[3] Woodcock SH, Benkendorff K. The impact of diet in the growth and proximate composition of juvenile whelks Dicathais orbita (Gastropoda: Mollusca). Aquaculture. 2008;276(1–4):162–170.

[4] Lev E, Zohar A. Practical Materia Maedicao of the Medieval Mediterranean According to the Cairo genizah. Vol. 7. Leiden, The. Netherlands: brill Online Books and Journals; 2008:664.

[5] Ciavatta ML, Lefranc F, Carbone M, et al. Marine mollusk – derived agents with antiproliferative activity as promising anticancer agents to overcome chemotherapy resistance. Med Res Rev. 2017;37(4):702–801.

[6] Dar HY, Lone Y, Koiri RK, Mishra PK, Srivastava RK. Microcystin-leucine arginine (MC-LR) induces bone loss and impairs bone micro-architecture by modulating host immunity in mice: implications for bone health. Environ Pollut. 2018;238:792–802.

[7] Hasegawa H, Mizoguchi I, Chiba Y, Ohashi M, Xu M, Yoshimoto T. Expanding diversity in molecular structures and functions of the IL-6/IL-12 heterodimeric cytokine family. Front Immunol. 2016;7:479.

[8] Watford WT, Hissong BD, Bream JH, Kanno Y, Muul L, O’Shea JJ. Signaling by IL-12 and IL-23 and the immunoregulatory roles of STAT4. Immunol Rev. 2004;202: 139–156.

[9] Hoeve MA, Savage ND, de Boer T, et al. Divergent effects of IL-12 and IL-23 on the production of IL-17 by human T cells. Eur J Immunol. 2006;36(3):661–670.

[10] Subbarao NV. Indian sea shells Part I: polyplacophora and gastropods. Rec. Zoo. Sur. Ind. 2003;416:96.

[11] Chellaram C, Edward JKP. Anti – nociceptive assets of coral associated gastropod, Drupa margariticola. Int J Pharm. 2009;5(3):236–239.

[12] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem. 1951;193(1):265–275.

[13] Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem. 1956;28(3):350–356.

[14] Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem. 1957;226(1):497–509.

[15] AOAC. In: Official Method of Analytical Chemists. 17th ed. Association of Official Analytical Chemists; 2000.

[16] Iqbal S, Kazmi F, Asad S, Mumtaz M, Khan AA. Dental Caries and diabetes mellitus. Pakistan Oral & Dental [journal]. 2011;31(1).

[17] Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966;45(4_ts):493–496.

[18] Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti – inflammatory activity of methanol extract of Oxalis corniculate Linn. Int J Pharm Sci. 2010;2: 146–155.

[19] Guan HS, Wang SG, Yang H, Dong WJZ, Yao W. In: Hai Ben Cao ZH, ed. [Chinese Marine Materia Medica. Shanghai]. Sci. Technol. Pub. House. 2009.

[20] Dinis TCP, Maderia VMC, Almeida LM. Action of phenolic derivatives (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys. 1994;315(1): 161–169.

[21] Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999;269(2):337–341.

[22] Jayasri MA, Radha A, Mathew TL. A- amylase and α-glucosidase inhibitory activity of Costus pictus D. Don in the management of diabetes. J Herb Toxicol. 2009;3:91–94.

[23] Jayasheela K, Nagabalasubramanian PB, Periandy S. Conformational and spectroscopic characterization, charge analysis and molecular docking profiles of chromone-3-carboxylic acid using a quantum hybrid computational method, 1-5: e04775 Heliyon. 2020;6(10).

[24] Babu M, Bundalovic-Torma C, Calmettes C, et al. Global landscape of cell envelope protein complexes in Escherichia coli. Nat Biotechnol. 2018;36(1):103–112.

[25] Trott O, Olson AJ. AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading, 30 J Comput Chem. 2010;31(2):455–461.

[26] Liu X, Zhang B, Jin Z, Yang H, Rao Z. The Crystal Structure of COVID-19 Main Protease in Complex with an Inhibitor. N3. Protein Data Bank; 2020.

[27] Sikorski ZE, Kolakowska A, Pan BS. The nutritive composition of the major groups of marine food organisms. In: Sikorki ZE, ed. Seafood: Resources, Nutritional Composition and Preservation. CRC Press; 1990:29–54.

[28] Lipton AP, Syda RG, Jagadis I. The Indian Sacred Chank. Marine Fisheries Research Institute; 2013, 978-93-82263-00-5.

[29] Periyasamy N, Srinivasan M, Devanathan K, Balakrishnan S. Nutritional value of gastropod babylonia spirata (linnaeus, 1758) from thazhanguda, southeast coast of India. Asian Pac J Trop Biomed. 2011;1(2):S249–S252.

[30] Benkendorff K, Rudd D, Nongmaithem BD, et al. Are the traditional medical uses of Muricidae molluscs substantiated by their pharmacological properties and bioactive compounds? Mar Drugs. 2015;13(8):5237–5275.

[31] Prem Anand T, Chellaram C, Chandrika M, et al. Nutritional studies on marine mollusk Pleuroploca trapezium (Gastropoda: fasciolariidae) from Tuticorin coastal waters. J Chem Pharm Res. 2013;5(4):16–21.

[32] Mereen JE, Diaz JHJ. GC – MS analysis and anti-inflammatory activity of Murex tribulus. Int J Pharm Sci Res. 2019;11(12):6184–6188.

[33] Correia AD, Costa MH, Luis OJ, Livingstone DR. Age – related changes in antioxidant enzyme activities, fatty acid composition and lipid peroxidation in whole body Gammarus locusta (Crustacea: Amphipoda). J Exp Mar Biol Ecol. 2003;289(1): 83–101.

[34] Nawata H. An export item from Badi on the western Red Sea Coast in the eighth century: historical and ethnographical studies on operculum as incense and perfume. Int. Conf. Ethiop Stud. 1997;3:307–325.

[35] Chellaram CM, Patterson EJ. Antibacterial activity of the winged oyster Pteria chinensis (Pterioida pteridae). Ind J Mar Sci. 2004;33:369–372.

[36] Jkp Edward , Ayyakkannu K. Shell trade and marketing with special reference to Chicoreus ramosus along the southeast coast of India. Phuket Mar Biol Cent Spec Publ. 1992:33–34.

[37] Kumar JKK, Prasad AGD. Identification and comparison of biomolecules in medicinal plants of Tephrosia tinctoria and Atylosia albicans by using FTIR. J Biophys. 2011;21: 63–71.

[38] Hashimoto A, Kameoka T. Applications of infrared spectroscopy to biochemical, food and agricultural processes. Appl Spectrosc Rev. 2008;43(5):416–451.

[39] Sahu N, Saxena J. Phytochemical analysis of bougainvillea glabra, choisy by FTIR and UV-vis spectroscopic analysis. Int J Pharm Sci Rev Res. 2013;21(1):196–198.

[40] Jamuna S, Paulsamy S. GC – MS analysis for bioactive compounds in the methanolic leaf and root extract of Hypochearis radicata L. (Asteraceae). Int J Curr Res. 2013; 5(12):4070–4074.

[41] Kim DH, Park MH, Choi YJ, et al. Molecular study of dietary heptadecane for the anti-inflammatory modulation of NF-kB in the aged kidney. PLoS One. 2013;8(3): e59316.

[42] Ten Brink T, Exner TE. Influence of protonation, tautomeric, and stereoisomeric states on protein–ligand docking results. J Chem Inf Model. 2009;49(6):1535–1546.

[43] Chopade AR, Mulla WA. Novel strategies for the treatment of inflammatory hyperalgesia. Eur J Clin Pharmacol. 2010;66(5):429–444.

RIGHTS & PERMISSIONS

2024 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.