Formulation, development and evaluation of Woodfordia fruticosa and β-sitosterol mediated silver nanoparticles gel for synergistic effect

Abstract

Background: Silver nanoparticles (AgNP) are frequently utilized metallic nanoparticles in healthcare systems. In this investigation, AgNP was produced employing β-sitosterol and Woodfordia fruticosa gel for the purpose of ameliorating wound healing.

Methods: The characterization of AgNP was conducted via SEM and spectrophotometry analysis. Simultaneously, a solution with a concentration of 1 mM of silver nitrate was prepared by dissolving 16.99 mg of silver nitrate in 100 ml of distilled water. Subsequently, the API and the silver nitrate solution were utilized in the synthesis of silver nanoparticles. The physicochemical properties, stability, and morphology of the synthesized silver nanoparticles were evaluated through various techniques, such as zeta potential analysis and electron microscopy. These techniques were employed for the purpose of assessing the aforementioned properties. Following the gel preparation, an assessment of the gel was performed.

Result: The investigation successfully produced silver nanoparticles (AgNP) using β-sitosterol and Woodfordia fruticosa gel. To characterize the synthesized AgNP, various techniques were employed, including SEM and spectrophotometry analysis. A 1 mM solution of silver nitrate was prepared and used in the synthesis process, and the physicochemical properties, stability, and morphology of the AgNP were assessed through zeta potential analysis and electron microscopy.

Conclusion: The results of this study indicate the successful synthesis of silver nanoparticles using β-sitosterol and Woodfordia fruticosa gel. The characterization techniques, including SEM and spectrophotometry, confirmed the formation of AgNP. The analysis of physicochemical properties and stability, particularly through zeta potential analysis, provided valuable insights into the stability of the synthesized nanoparticles. These findings have significant implications in the context of wound healing. Silver nanoparticles have been widely studied for their potential wound healing properties due to their antimicrobial and anti-inflammatory characteristics. The utilization of natural compounds like β-sitosterol and botanical sources like Woodfordia fruticosa gel for the synthesis of AgNP is particularly promising as it may reduce potential toxicity and enhance the biocompatibility of these nanoparticles.

Keywords

Silver nanoparticles / β-sitosterol / Woodfordia fruticosa / Zeta potential analysis / Wound healing / Synthesized nanoparticles

References

[1] Singh A, Ansari VA, Haider F, et al. Oral fast dissolving film: the avant-garde avenue for oral consignment modus operandi. Res J Pharm Technol. 2021;14(4):2145–2152.

[2] Maheshwari S, Tiwari RK, Singh L. Green expertise: synthesis of Silver nanoparticles for wound healing application an overview. Res J Pharm Technol. 2021 Feb 1;14(2):1149–1154.

[3] Singh A, Maheshwari S, Vishwakarma VK, Mohammed S. Processed excipients for targeted drug delivery in cancer management: enhancing efficacy and precision. Intelligent Pharm. 2023 Oct 17.

[4] Maheshwari S. Synergistic effects of Woodfordia fruticosa silver nanoparticles accelerating wound healing in Swiss mice in vivo. Intelligent Pharm. 2023 Oct 3.

[5] Singh A, Maheshwari S. Extraction, development, and characterization of tamarind seed oil based liposome. Int J Pharmaceutical Res. 2020 Jul 1;12(3).

[6] Maheshwari S, Singh A. Development and evaluation of topical liposomal gel of lemon oil and orange oil for synergistic effect to enhance skin beauty. Int J Pharm Professional’s Res (IJPPR). 2023;14(2):91–102.

[7] Maheshwari S, Tiwari RK, Singh L. Novel drug delivery: transdermal patches. Int J Pharmaceutical Res. 2020 Jul 1;12(3):09752366.

[8] Singh A, Ansari VA, Ahsan F, Akhtar J, Khushwaha P, Maheshwari S. Viridescent concoction of genstein tendentious silver nanoparticles for breast cancer. Res J Pharm Technol. 2021;14(5):2867–2872.

[9] Singh A, Ansari VA, Haider MF, Ahsan F, Mahmood T, Wasim R. Development and evaluation of topical liposome preparation of flax seed oil and tamarind seed oil against skin-protective activity. Pharmaceut Chem J. 2023 Oct 17:1–4.

[10] Najda A, Bains A, Chawla P, et al. Assessment of anti-inflammatory and antimicrobial potential of ethanolic extract of Woodfordia fruticosa flowers: GC-MS analysis. Molecules. 2021 Nov 27;26(23):7193.

[11] Elbahnasawy MA, Shehabeldine AM, Khattab AM, Amin BH, Hashem AH. Green biosynthesis of silver nanoparticles using novel endophytic Rothia endophytica: characterization and anticandidal activity. J Drug Deliv Sci Technol. 2021 Apr 1;62:102401.

[12] Lokesh BS, Haloi P, Konkimalla VB. Fabrication and optimization of BSA-PEG-loaded phenethyl isothiocyanate (PEITC) nanoparticles using Box-Behnken design for potential application in subcutaneous infection condition. J Drug Deliv Sci Technol. 2023 Feb 1;80:104101.

[13] Naikwadi PH, Phatangare ND, Mane DV. Active anti-inflammatory potency of γ-sitosterol from woodfordia floribunda salisb. J Plant Sci Res. 2022 Jul 1;38(2).

[14] Patel SP, Vaishya R, Mishra GP, Tamboli V, Pal D, Mitra AK. Tailor-made pentablock copolymer based formulation for sustained ocular delivery of protein therapeutics. J drug delivery. 2014;2014.

[15] Rodrigues MA, Cortez AR, Cortez J, et al. Antimicrobial profile of a dental implant abutment coating to prevent adhesion and migration of bacteria and screw loosening. Dent Mater. 2021 Oct 1;37(10):e493–e501.

[16] Dieny FF. Hasil Review Association of Dietary Index with Visceral Adiposity Index Among Obese.

[17] Aulia RN, Sriwidodo S. Sistem penghantaran obat ekstrak herbal dalam formulasi dan aplikasi biomedis. Majalah Farmasetika. 2022 Jul 1;7(5).

[18] Raota CS, Cerbaro AF, Salvador M, et al. Green synthesis of silver nanoparticles using an extract of Ives cultivar (Vitis labrusca) pomace: characterization and application in wastewater disinfection. J Environ Chem Eng. 2019 Oct 1;7(5):103383.

[19] Marinescu L, Ficai D, Ficai A, et al. Comparative antimicrobial activity of silver nanoparticles obtained by wet chemical reduction and solvothermal methods. Int J Mol Sci. 2022 May 26;23(11):5982.

[20] Menazea AA, Abdelghany AM. Precipitation of silver nanoparticle within silicate glassy matrix via Nd: YAG laser for biomedical applications. Radiat Phys Chem. 2020 Sep 1;174:108958.

[21] Shejawal KP, Randive DS, Bhinge SD, et al. Green synthesis of silver, iron and gold nanoparticles of lycopene extracted from tomato: their characterization and cytotoxicity against COLO320DM, HT29 and Hella cell. J Mater Sci Mater Med. 2021 Feb;32:1–2.

[22] Krzyszczyk P, Schloss R, Palmer A, Berthiaume F. The role of macrophages in acute and chronic wound healing and interventions to promote pro-wound healing phenotypes. Front Physiol. 2018 May 1;9:419.

[23] Ellis S, Lin EJ, Tartar D. Immunology of wound healing. Curr Dermatol Rep. 2018 Dec;7:350–358.

[24] Metcalf DG, Bowler PG. Biofilm delays wound healing: a review of the evidence. Burns & Trauma. 2013 Jun;1(1):2321–3868.

[25] Fandrich R, Gu Y, Burrows D, Moeller K. Modern SEM-based mineral liberation analysis. Int J Miner Process. 2007 Oct 19;84(1–4):310–320.

[26] Purwanto A, Sudargini Y. Partial least squares structural squation modeling (PLS-SEM) analysis for social and management research: a literature review. J Industrial Eng & Manag Res. 2021 Jul 4;2(4):114–123.

[27] Mohammed A, Abdullah A. Scanning electron microscopy (SEM): a review. In: InProceedings of the 2018 International Conference on Hydraulics and Pneumatics—HERVEX, Băile Govora, Romania. 2018 Nov 7:7–9, 2018.

[28] Gallagher W. FTIR analysis of protein structure. Course manual Chem. 2009 Oct 26:455.

[29] Carrillo F, Colom X, Sunol JJ, Saurina J. Structural FTIR analysis and thermal characterisation of lyocell and viscose-type fibres. Eur Polym J. 2004 Sep 1;40(9):2229–2234.

[30] Liu Q, Wang S, Zheng Y, Luo Z, Cen K. Mechanism study of wood lignin pyrolysis by using TG–FTIR analysis. J Anal Appl Pyrol. 2008 May 1;82(1):170–177.

[31] Bagur H, Medidi RS, Somu P, et al. Endophyte fungal isolate mediated biogenic synthesis and evaluation of biomedical applications of silver nanoparticles. Mater Technol. 2022 Feb 23;37(3):167–178.

[32] Huang Y, Bai L, Yang Y, Yin Z, Guo B. Biodegradable gelatin/silver nanoparticle composite cryogel with excellent antibacterial and antibiofilm activity and hemostasis for Pseudomonas aeruginosa-infected burn wound healing. J Colloid Interface Sci. 2022 Feb 15;608:2278–2289.

[33] Abdellatif AA, Tolba NS, Al Rugaie O, Alhumaydhi FA, Mousa AM. Green synthesis of silver nanoparticles for enhancing wound healing activity in rats. Saudi Pharmaceut J. 2022;10.

[34] Mostafa M, Kandile NG, Mahmoud MK, Ibrahim HM. Synthesis and characterization of polystyrene with embedded silver nanoparticle nanofibers to utilize as antibacterial and wound healing biomaterial. Heliyon. 2022 Jan 1;8(1):e08772.

[35] Liu Q, Zhang Y, Huang J, et al. Mesoporous silica-coated silver nanoparticles as ciprofloxacin/siRNA carriers for accelerated infected wound healing. J Nanobiotechnol. 2022 Aug 23;20(1):386.

[36] Cataldi S, Ceccarini MR, Patria F, et al. The effect of vitamin D3 and silver nanoparticles on HaCaT cell viability. Int J Mol Sci. 2022 Jan 26;23(3):1410.

[37] Rathore HS, Sivagnanam UT, Abraham LS, Prakash D, Panda RC, Senthilvelan T. Green synthesized silver nanoparticles-impregnated novel gum kondagogu-chitosan biosheet for tissue engineering and wound healing applications. Polym Bull. 2022 Sep;79(9):7215–7227.

[38] Zhou A, Zhang Y, Zhang X, et al. Quaternized chitin/tannic acid bilayers layer-by-layer deposited poly (lactic acid)/polyurethane nanofibrous mats decorated with photoresponsive complex and silver nanoparticles for antibacterial activity. Int J Biol Macromol. 2022 Mar 15;201:448–457.

[39] Raghuwanshi N, Kumari P, Srivastava AK, et al. Synergistic effects of Woodfordia fruticosa gold nanoparticles in preventing microbial adhesion and accelerating wound healing in Wistar albino rats in vivo. Mater Sci Eng C. 2017 Nov 1;80:252–262.

[40] Desai AS, Singh A, Edis Z, et al. An in vitro and in vivo study of the efficacy and toxicity of plant-extract-derived silver nanoparticles. J Funct Biomater. 2022 May 10;13(2):54.

[41] Kabeerdass N, Murugesan K, Arumugam N, et al. Biomedical and textile applications of alternanthera sessilis leaf extract mediated synthesis of colloidal silver nanoparticle. Nanomaterials. 2022 Aug 12;12(16):2759.

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