Development And Evaluation of topical liposomal preparation of Walnut oil and tamarind seed oil against ageing

Abstract

Background: Revamp of liposomal preparation inimical ageing by using a splendid treasure of herbal drugs includes non-prescription and prescription upshot. Liposomal therapy stands at the forefront of cutting-edge cosmeceutical innovation. It involves a sophisticated, bi-layered delivery system designed for the precise infusion of walnut oil and tamarind seed oil into the skin. This innovative approach effectively combats the signs of ageing, whether they are the result of a sedentary lifestyle or genetic factors within one’s family history.

Purpose: The current study focuses on exploring the area of traditional systems of medicine in the field of pharmaceuticals to achieve the combined effect of walnut oil [F1] and tamarind seed oil [F2] in the form of liposomal preparation against ageing.

Methods: Liposomes of walnut oil and tamarind seed oil were developed by using a rotary vacuum evaporator, and microscopy of the formulated liposomes was done using a Digicam microscope, which provided a microscopic view that both formulated liposomes are spherical, but F1 liposomes are more dense and tactile than F2, and the F2 liposomal formulation has more vesicles than F1. In-vitro drug release studies of F1 and F2 were done by diffusion cells while using a dialysis membrane with buffer medium 6.8. Further characterization techniques like SEM and FTIR were used to investigate the size range with the clear shape of the particles and to obtain a clear peak that matched the pure drug, which shows that it has retained its pharmacological properties. The final step was directed towards the preparation of topical anti-ageing cream by combining F1 and F2 to obtain the synergistic effect, and the formulation cream was successfully visualised under a projection microscope at a magnification of 10x, which reveals that spherically droplet vacuoles are present with a soft texture and a creamy, whitish colour. Last but not least, a 14-day animal study was conducted on female Swiss mice from the authorised animal laboratory departmental animal house at Integral University. The studies were conducted in 3 groups: G1 was given saline as a control, G2 was given Olay as a topical application, and G3 was given the test cream. The anti-ageing potential of the formulated cream was evaluated by skin compliance studies using UV exposure.

Results: Walnut oil and tamarind seed oil-based liposomes were formulated after placebo selection, i.e., F1 and F2, and the microscopic view of F1 sphere-shaped vesicles is larger, brighter, and more dense than F2, and the sphereshaped vesicles of F1 and F2 contain phospholipid bilayers composed of soyalecthin, which helps to deliver drugs at a specific site. The pH of two formulations, F1 and F2, was determined by storing them at different temperatures, 350°C and 450°C. The pH resultant values were in the range of 5.8 to 6.9, which means that the formulated liposomes are ideal for skin function. In-vitro drug release studies of F1 and F2 data revealed that F1 shows less drug release than F2 at 35 min, which is less than 100%. This specified study was done to identify the time that was taken to release the drug. The structural analysis of liposomes F1 and F2 was done by the highly characterised technique of SEM, and the result revealed that F1 and F2 were in a spherical shape. F1 liposome was 5 µm in diameter, while F2 liposome was 1 µm in diameter, which means that they lie in the bilayer liposomal range. FTIR is an important parameter to analyse the functional group and also to identify the peak of the pure drug that is similar to the excipient for retaining the pharmacological action, so here F1 and F2 liposomes are similar to the pure drug. The topical preparation of liposomes F1 and F2 was decided to formulate cream that is easy to use and store. The combining anti-ageing creams of F1 and F2 were visualised under a projection microscope, and the view of the liposomal cream revealed sphere-shaped vesicles. As a result of skin compliance studies, **G3 is significantly more significant than G1 and *G2 is significantly more significant than G1. The antiageing evaluation study stated that **G3 is much more significant than G2, and *G2 is much more significant than G1.

Conclusion: The present study indicates that walnut oil and tamarind seed oil were successfully entrapped in a liposomal formulation in the form of cream. The characterization studies reveal that they are potent against ageing.

Keywords

Ageing / Topical application / Liposomal / Compliance / Exposed

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