An aqueous extract of Syzygium cumini bark reduces the rate of preterm birth in rats

Abstract

Preterm delivery is a considerable burden on prenatal healthcare and a major risk factor for neurological impairment and disability. A study looked at whether an aqueous extract of Syzygium cumini (AESC) bark could stop rats from giving birth too early. The DPPH and ABTS radical scavenging assays were used to evaluate the antioxidant activity of AESC in vitro, and the results showed that this extract possesses free radical scavenging activity, which helps to prevent premature labour. A piece of an isolated rat uterus was used for in-vitro pharmacological testing of the AESC at 25 mg/ml and 50 mg/ml. The extract showed free radical scavenging activity, effectively suppressing uterine contractions by 45.7% and 66.9%, respectively. The researchers concluded that AESC has considerable tocolytic activity, resulting in a decrease in the rate of premature birth. The extract showed no significant adverse effects on mothers or their infants, suggesting it may be a safe option for pregnant individuals seeking to prevent preterm labor. The extract also exhibited a dose-dependent effect, with higher doses resulting in a greater reduction in premature birth rates, further supporting its efficacy as a tocolytic agent.

Keywords

Syzygium cumini / Tocolytic activity / Antioxidant activity / Preterm labor / Rat uterus muscle

References

[1] Tantratian S, Krusong W, Siriwetwut O. Combination of Syzygium cumini (L) Skeels seed extract with acetic acid to control Escherichia coli on mint (Mentha cordifolia opiz.) leaves. Lebensm Wiss Technol. 2022;164:113619.

[2] Parimala SM, Salomi AA. GC-MS analysis and antimicrobial assessment of syzygium cumini (L.) skeels seed ethanol extract. J of Pharm Res Inter. 2021:271–283.

[3] Magalhães A, Melo G, Gabriel N, et al. Susceptibility of animal pathogenic bacteria to an ethanolic extract from jambolan (Syzygium cumini) leaves. Planta Med. 2015;81.

[4] Shankar P, Singh RV, Kumar A. Therapeutic protection of arsenic-induced oxidative stress and hepato-nephro toxicity by Syzygium cumini (seed) ethanolic extract (SCEE) in charles foster rats. Inter. 2023:207–224.

[5] Lyell DJ, El-Sayed YY. Magnesium sulfate compared with nifedipine for acute tocolysis of preterm labor: a randomized controlled trial. Obstet Gynecol. 2007;110:1171.

[6] Abbas F. Artificial neural network (ANN) approach for modeling of methyl orange adsorption by Syzygium cumini seed coat. Pure and App Bio. 2021;10.

[7] Sivaprakasam M. An in vitro study of syzygium cumini seed extract on glucose uptake activity in l-6 cell lines. J Drug Deliv Therapeut. 2019;9:256–259.

[8] Sebastian A, George AM. Evaluation of tocolytic activity of aqueous seed extract of syzygium cumini on oxytocin induced preterm labor. Asian J Pharmaceut Clin Res. 2020:162–170.

[9] Supraba W, Juliantoni Y, Ananto AD. The effect of stirring speeds to the entrapment efficiency in a nanoparticles formulation of java plumâ seed ethanol extract (syzygium cumini). Acta Chimica Asia. 2021;4:197–203.

[10] Babu PS, Krishnan G, Babu KA, Chitra K. In silico and in vitro evaluation of antiurolithiatic activity of ethanolic extract of syzygium cumini stem bark. Res J Pharm Technol. 2017;10:1317.

[11] Rohadi R, Santoso U, Raharjo S, Falah II. Determination of antioxidant activity and phenolic compounds of methanolic extract of java plum (syzygium cumini linn. (Skeel) seed. Indo Food and Nut Prog. 2017;14:9.

[12] Asanaliyar M, Nadig P. In -vivo anti-diabetic activity of hydro-ethanolic seed extract of syzygium cumini (L.). Biomed Pharmacother J. 2021;14:241–247.

[13] Puspitasari L, Dira MA. Phytochemical screening and antidiabetic activities test of ethanol extract from Syzygium cumini L. seeds in male Wistar rats induced by alloxan. Pharm Educ. 2022:165–168.

[14] Yilmaz O, Göncü AŞ. Effects of nifedipine on fetal cardiac function in preterm labor. J Perinat Med. 2020;48:723–727.

[15] Jaju PB. Effectiveness and safety of isoxsuprine hydrochloride as tocolytic agent in arresting active/threatened preterm labor and its role in maintenance tocolysis: a prospective, open-label study. Am Jails. 2019;38:291–295.

[16] Ahmadvand H. Inhibitory effects of Oak fruit (Quercus) husks hydroalcoholic extract on LDL oxidation in vitro. Clin BioMech. 2011;44:S341.

[17] Prasad M, Venugopal SP. The role of ethanolic extract of syzygium cumini stem bark on female reproductive system in wistar rats. Int J Anat Res. 2020;8:7835–7840.

[18] Anas M, Malik A. Impact of sodium alginate packaging film synthesized using syzygium cumini seed extract on multi drug resistant Escherichia coli isolated from raw Buffalo meat. Indian J Microbiol. 2021;61:137–150.

[19] Aziz A, Banerjee S. Phytochemical screening and antibacterial activity study of syzygium cumini (myrtaceae) seed extracts. Pharm Times. 2018;6:70.

[20] Sungkar S, Haniastuti T, Santoso AS, Agustina D. The effect of ethanolic extract of syzygium cumini leaves on the growth of Streptococcus mutans. Dentika: Dent J. 2018;21:32–36.

[21] Asanaliyar M, Nadig P. In -vivo anti-diabetic activity of hydro-ethanolic seed extract of syzygium cumini (L.). Biomed Pharmacother J. 2021;14:241–247.

[22] Prajapati M, Mohanty PK, Rai JP. Antiulcer activity of hydroalcoholic seed extract of moringa oleifera lam. and syzygium cumini (l.) skeels in pylorus ligation induced and ethanol induced gastric ulcer in rats. J of Ad Scientific Res. 2021;12:102–106.

[23] Rathod M, Rakholiya K, Kaneria M. Effects of extraction techniques on phytochemical analysis and antioxidant activity of syzygium cumini (L.) skeels seeds. SSRN Electron J.2020.

[24] Monteiro FS, Carvalho AFS, Ribeiro RM, Borges ACR, Borges MOR. Phytochemical profile and investigation of the spasmolytic activity of hydroalcoholic extract of syzygium cumini (L.) skeels seeds. Eur J Med Plants. 2020:27–38.

[25] Tantratian S, Krusong W, Siriwetwut O. Combination of Syzygium cumini (L) Skeels seed extract with acetic acid to control Escherichia coli on mint (Mentha cordifolia opiz.) leaves. Lebensm Wiss Technol. 2022;164:113619.

[26] Bayrami G, Boskabady MH, Iranshahi M, Gholamnezhad Z. Relaxant effects of asafoetida extract and its constituent umbelliprenin on Guinea-pig tracheal smooth muscle. Chin J Med.2013.

[27] Venkatasamy R, Spina D. Novel relaxant effects of RPL554 on Guinea pig tracheal smooth muscle contractility. Br J Pharmacol. 2016;173:2335–2351.

[28] Özay C, Mammadov R. Antioxidant activity, total phenolic, flavonoid and saponin contents of different solvent extracts of C. Phrygius bornm. Cur Per on Med and Aroma Plant. (CUPMAP). 2019;2:23–28.

[29] Ghasemi Pirbalouti A, Siahpoosh A, Setayesh M, Craker L. Antioxidant activity, total phenolic and flavonoid contents of some medicinal and aromatic plants used as herbal teas and condiments in Iran. J Med Food. 2014;17:1151–1157.

[30] Mere JK, Bintang M, Safithri M. Antibacterial effectiveness of syzygium cumini (L.) skeels leaves to Escherichia coli pBR322. Indo J Chem Res. 2021;9:8–14.

[31] Tantratian S, Krusong W, Siriwetwut O. Combination of Syzygium cumini (L) Skeels seed extract with acetic acid to control Escherichia coli on mint (Mentha cordifolia opiz.) leaves. Lebensm Wiss Technol. 2022;164:113619.

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