Chromosome-level genome assembly of the Asian citrus psyllid, Diaphorina citri
Received date: 09 Mar 2023
Revised date: 13 Apr 2023
Accepted date: 17 Apr 2023
Copyright
Diaphorina citri is a global citrus pest. As a vector insect, it can transmit the causative agents of citrus huanglongbing, causing irreversible losses to the citrus industry. The acquisition of genomic information can provide a molecular genetic basis for effective control of D. citri. Here, the DNBSEQ™, Oxford Nanopore Technologies, and Hi-C technologies are applied to generate a high-quality chromosome-level genome of D. citri. The genome size of D. citri was 523.78 Mb with a scaffold N50 of 47.05 Mb distributed on 13 chromosomes. A total of 250.64 Mb (47.85%) repeat sequences and 24 048 protein-coding genes were predicted. Genome resequencing of female and male individuals indicated that the sex chromosome system of D. citri is XO. Phylogenetic analysis demonstrated that D. citri and Pachypsylla venusta, which separated from their most recent common ancestor about 336.62 million years ago, were the most closely related. Additionally, we identified genes potentially involved in detoxification metabolism, pathogen transmission, and honeydew secretion for further investigation. The high-quality genome provides an important reference for developing effective management strategies of D. citri.
Shuang Lei , Shi-Jiang Yu , Qi Pan , Li-Li Ding , Si-Chen Li , Lu-Yan Cheng , Shu-Qi Wang , Bing-Hai Lou , Jun He , Cui-Yun Lei , Lin Cong , Hao-Qiang Liu , Xue-Feng Wang , Chun Ran . Chromosome-level genome assembly of the Asian citrus psyllid, Diaphorina citri[J]. Insect Science, 2024 , 31(1) : 13 -27 . DOI: 10.1111/1744-7917.13214
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