Insect Science >

2024 , Vol. 31 >Issue 1: 13 - 27

DOI: https://doi.org/10.1111/1744-7917.13214

Chromosome-level genome assembly of the Asian citrus psyllid, Diaphorina citri

  • Shuang Lei 1 ,
  • Shi-Jiang Yu 1 ,
  • Qi Pan 1 ,
  • Li-Li Ding 1 ,
  • Si-Chen Li 1 ,
  • Lu-Yan Cheng 1 ,
  • Shu-Qi Wang 1 ,
  • Bing-Hai Lou 2 ,
  • Jun He 1 ,
  • Cui-Yun Lei 2 ,
  • Lin Cong 1 ,
  • Hao-Qiang Liu 1 ,
  • Xue-Feng Wang , 1 ,
  • Chun Ran , 1
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  • 1. Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, National Engineering Research Center for Citrus, Chongqing, China
  • 2. Guangxi Key Laboratory of Citrus Biology, Guangxi Academy of Specialty Crops, Guilin, Guangxi, China
wangxuefeng@cric.cn
ranchun@cric.cn

Received date: 09 Mar 2023

Revised date: 13 Apr 2023

Accepted date: 17 Apr 2023

Copyright

2023 2023 Institute of Zoology, Chinese Academy of Sciences.
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Abstract

Diaphorina citri is a global citrus pest. As a vector insect, it can transmit the causative agents of citrus huanglongbing, causing irreversible losses to the citrus industry. The acquisition of genomic information can provide a molecular genetic basis for effective control of D. citri. Here, the DNBSEQ™, Oxford Nanopore Technologies, and Hi-C technologies are applied to generate a high-quality chromosome-level genome of D. citri. The genome size of D. citri was 523.78 Mb with a scaffold N50 of 47.05 Mb distributed on 13 chromosomes. A total of 250.64 Mb (47.85%) repeat sequences and 24 048 protein-coding genes were predicted. Genome resequencing of female and male individuals indicated that the sex chromosome system of D. citri is XO. Phylogenetic analysis demonstrated that D. citri and Pachypsylla venusta, which separated from their most recent common ancestor about 336.62 million years ago, were the most closely related. Additionally, we identified genes potentially involved in detoxification metabolism, pathogen transmission, and honeydew secretion for further investigation. The high-quality genome provides an important reference for developing effective management strategies of D. citri.

Key words： Asian citrus psyllid; chromosome-level genome; comparative genomics; cytochrome P450; detoxification; Huanglongbing

Cite this article

Shuang Lei , Shi-Jiang Yu , Qi Pan , Li-Li Ding , Si-Chen Li , Lu-Yan Cheng , Shu-Qi Wang , Bing-Hai Lou , Jun He , Cui-Yun Lei , Lin Cong , Hao-Qiang Liu , Xue-Feng Wang , Chun Ran . Chromosome-level genome assembly of the Asian citrus psyllid, Diaphorina citri[J]. Insect Science, 2024 , 31(1) : 13 -27 . DOI: 10.1111/1744-7917.13214

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