Force-induced Caspase-1-dependent pyroptosis regulates orthodontic tooth movement

Liyuan Chen1, Huajie Yu2, Zixin Li1, Yu Wang1, Shanshan Jin1, Min Yu1, Lisha Zhu1, Chengye Ding1, Xiaolan Wu1, Tianhao Wu1, Chunlei Xun1, Yanheng Zhou1, Danqing He1, Yan Liu1

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International Journal of Oral Science ›› 2024, Vol. 16 ›› Issue (0) : 3. DOI: 10.1038/s41368-023-00268-7

Force-induced Caspase-1-dependent pyroptosis regulates orthodontic tooth movement

  • Liyuan Chen1, Huajie Yu2, Zixin Li1, Yu Wang1, Shanshan Jin1, Min Yu1, Lisha Zhu1, Chengye Ding1, Xiaolan Wu1, Tianhao Wu1, Chunlei Xun1, Yanheng Zhou1, Danqing He1, Yan Liu1
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Abstract

Pyroptosis, an inflammatory caspase-dependent programmed cell death, plays a vital role in maintaining tissue homeostasis and activating inflammatory responses. Orthodontic tooth movement (OTM) is an aseptic force-induced inflammatory bone remodeling process mediated by the activation of periodontal ligament (PDL) progenitor cells. However, whether and how force induces PDL progenitor cell pyroptosis, thereby influencing OTM and alveolar bone remodeling remains unknown. In this study, we found that mechanical force induced the expression of pyroptosis-related markers in rat OTM and alveolar bone remodeling process. Blocking or enhancing pyroptosis level could suppress or promote OTM and alveolar bone remodeling respectively. Using Caspase-1-/- mice, we further demonstrated that the functional role of the force-induced pyroptosis in PDL progenitor cells depended on Caspase-1. Moreover, mechanical force could also induce pyroptosis in human ex-vivo force-treated PDL progenitor cells and in compressive force-loaded PDL progenitor cells in vitro, which influenced osteoclastogenesis. Mechanistically, transient receptor potential subfamily V member 4 signaling was involved in force-induced Caspase-1-dependent pyroptosis in PDL progenitor cells. Overall, this study suggested a novel mechanism contributing to the modulation of osteoclastogenesis and alveolar bone remodeling under mechanical stimuli, indicating a promising approach to accelerate OTM by targeting Caspase-1.

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Liyuan Chen, Huajie Yu, Zixin Li, Yu Wang, Shanshan Jin, Min Yu, Lisha Zhu, Chengye Ding, Xiaolan Wu, Tianhao Wu, Chunlei Xun, Yanheng Zhou, Danqing He, …Yan Liu. Force-induced Caspase-1-dependent pyroptosis regulates orthodontic tooth movement. International Journal of Oral Science, 2024, 16(0): 3 https://doi.org/10.1038/s41368-023-00268-7

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