TET1 knockdown inhibits the odontogenic differentiation potential of human dental pulp cells

Abstract

A protein that promotes cell proliferation and differentiation may play a key role in dentin regeneration and repair. Human dental pulp cells (hDPCs) are multipotent stem cells found in the soft tissue inside teeth; they differentiate into other cell types to generate new dentin following injury or infection. Qiong Xu at Sun Yat-sen University in Guangzhou, China, and co-workers investigated a recently discovered protein called TET1 to determine its role in hDPCs during dentin repair. TET1 is known to be expressed in hDPCs, and has been shown to regulate differentiation in various other cell types. The team disabled the TET1 gene, which resulted in reduced expression of TET1 in hDPCs. The loss of TET1 appeared to decrease the hDPCs’ growth rate and prevented differentiation by reducing the expression of key genes and limiting mineralization.

Keywords

DNA demethylation / human dental pulp cell / knockdown / odontogenic differentiation / ten–eleven translocation 1

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