Engineered three-dimensional rabbit oral epithelial–mesenchymal–muscular hybrid sheets

Abstract

Laboratory-made sheets of the cells from the inside of the mouth could help treat oral muscular defects. Shigeki Yamane and colleagues from Tokyo Dental College, Japan, isolated different kinds of rabbit cells from the mucous membrane that lines the mouth. They then fabricated layered sheets that contained all three cell types: epithelial, mesenchymal, and muscular. The researchers studied the expression of proteins that are essential for maintaining this structure and found that, on a molecular level, the tissues of the engineered sheets closely resembled those found inside the oral cavity. The cells also had the capacity to proliferate after grafting. Similar constructs made of human cells could offer a path toward regenerative therapies for people with muscle problems in the tongue or pharynx who have trouble swallowing or chewing.

Keywords

mesenchymal stem cell / multi-differentiation / myoblast / oral mucosa / three-dimensional culture

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