PiggyBac transposon-mediated gene delivery efficiently generates stable transfectants derived from cultured primary human deciduous tooth dental pulp cells (HDDPCs) and HDDPC-derived iPS cells

Abstract

A method that enables the efficient, effective delivery of genes into human baby teeth cells could aid research into tooth regeneration. Dental pulp cells found inside baby teeth give rise to other cells that make dentin, one of the major components of teeth. Masahiro Sato from Kagoshima University, Japan, and colleagues now show that a gene delivery system called ‘PiggyBac’ can be used to introduce DNA into these pulp cells or stem cells derived from them, yielding modified cells that stably express one or two genes for months at a time. The PiggyBac system greatly outperforms other more conventional gene delivery systems, but because of safety risks is not suited for clinical application. Rather, the system is expected to boost functional studies of genes and proteins related to tooth development.

Keywords

drug selection / electroporation / genetically modified / human deciduous tooth dental pulp cells / PiggyBac

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