Anti-proteolytic capacity and bonding durability of proanthocyanidin-biomodified demineralized dentin matrix

Abstract

Plant-derived compounds called proanthocyanidins can protect tooth dentin from breakdown by enzymes. A team led by Ji-Hua Chen from the Fourth Military Medical University in Xi'an, China, treated human third molars with a grape-seed extract rich in proanthocyanidins, a naturally occurring plant compound. The researchers exposed slabs of dentin from these teeth to enzymes that break down collagen, an important structural protein in dentin. Collagen breakdown is one of the main reasons for the deterioration of resin-dentin bonds over time. They found that dentin specimens treated with proanthocyanidin exhibited lower levels of collagen breakdown and maintained higher bond strengths than non-treated control samples. The proanthocyanidin treatment also inhibited the dental tissue from producing gelatinases, enzymes that further break down collagen. This type of treatment could help strengthen the bonding of dentin for clinical applications.

Keywords

bonding durability / collagenolysis / crosslinking agents / dentin / proanthocyanidins

References

[1] Breschi L, Mazzoni A, Ruggeri A. Dental adhesion review: aging and stability of the bonded interface. Dent Mater, 2008, 24(1): 90-101.

[2] Liu Y, Tjaderhane L, Breschi L. Limitations in bonding to dentin and experimental strategies to prevent bond degradation. J Dent Res, 2011, 90(8): 953-968.

[3] Hashimoto M, Ohno H, Kaga M. In vivo degradation of resin–dentin bonds in humans over 1 to 3 years. J Dent Res, 2000, 79(6): 1385-1391.

[4] Breschi L, Martin P, Mazzoni A. Use of a specific MMP-inhibitor (galardin) for preservation of hybrid layer. Dent Mater, 2010, 26(6): 571-578.

[5] Breschi L, Mazzoni A, Nato F. Chlorhexidine stabilizes the adhesive interface: a 2-year in vitro study. Dent Mater, 2010, 26(4): 320-325.

[6] de Munck J, van den Steen PE, Mine A. Inhibition of enzymatic degradation of adhesive–dentin interfaces. J Dent Res, 2009, 88(12): 1101-1106.

[7] Ricci HA, Sanabe ME, de Souza Costa CA. Chlorhexidine increases the longevity of in vivo resin–dentin bonds. Eur J Oral Sci, 2010, 118(4): 411-416.

[8] Sadek FT, Braga RR, Muench A. Ethanol wet-bonding challenges current anti-degradation strategy. J Dent Res, 2010, 89(12): 1499-1504.

[9] La VD, Howell AB, Grenier D. Cranberry proanthocyanidins inhibit MMP production and activity. J Dent Res, 2009, 88(7): 627-632.

[10] Epasinghe DJ, Yiu CK, Burrow MF. The inhibitory effect of proanthocyanidin on soluble and collagen-bound proteases. J Dent, 2013, 41(9): 832-839.

[11] Castellan CS, Pereira PN, Grande RH. Mechnical characterization of proanthocyandin–dentin matrix interaction. Dent Mater, 2010, 26(10): 968-973.

[12] Castellan CS, Bedran-Russo AK, Karol S. Long-term stability of dentin matrix following treatment with various natural collagen cross-linkers. J Mech Behav Biomed Mater, 2011, 4(7): 1343-1350.

[13] Castellan CS, Bedran-Russo AK, Antunes A. Effect of dentin biomodification using naturally derived collagen cross-linkers: one-year bond strength study. Int J Dent, 2013, 2013: 918010.

[14] Hechler B, Yao X, Wang Y. Proanthocyanidins alter adhesive/dentin bonding strengths when included in a bonding system. Am J Dent, 2012, 25(5): 276-280.

[15] Liu R, Fang M, Xiao Y. The effect of transient proanthocyanidins preconditioning on the cross-linking and mechanical properties of demineralized dentin. J Mater Sci Mater Med, 2011, 22(11): 2403-2411.

[16] Fang M, Liu R, Xiao Y. Biomodification to dentin by a natural crosslinker improved the resin–dentin bonds. J Dent, 2012, 40(6): 458-466.

[17] Liu RR, Fang M, Zhao SJ. [The potential effect of proanthocyanidins on the stability of resin-dentin bonds against thermal cycling.]. Zhonghua Kou Qiang Yi Xue Za Zhi, 2012, 47(5): 268-272.

[18] Liu Y, Chen M, Yao X. Enhancement in dentin collagen’s biological stability after proanthocyanidins treatment in clinically relevant time periods. Dent Mater, 2013, 29(4): 485-492.

[19] Yamauchi M, Shiiba M. Lysine hydroxylation and cross-linking of collagen. Methods Mol Biol, 2008, 446: 95-108.

[20] Tezvergil-Mutluay A, Agee KA, Hoshika T. The inhibitory effect of polyvinylphosphonic acid on functional matrix metalloproteinase activities in human demineralized dentin. Acta Biomater, 2010, 6(10): 4136-4142.

[21] Mazzoni A, Mannello F, Tay FR. Zymographic analysis and characterization of MMP-2 and -9 forms in human sound dentin. J Dent Res, 2007, 86(5): 436-440.

[22] Hosaka K, Nishitani Y, Tagami J. Durability of resin–dentin bonds to water- vs. ethanol-saturated dentin. J Dent Res, 2009, 88(2): 146-151.

[23] Sulkala M, Tervahartiala T, Sorsa T. Matrix metalloproteinase-8 (MMP-8) is the major collagenase in human dentin. Arch Oral Biol, 2007, 52(2): 121-127.

[24] Perumal S, Antipova O, Orgel JP. Collagen fibril architecture, domain organization, and triple-helical conformation govern its proteolysis. Proc Natl Acad Sci U S A, 2008, 105(8): 2824-2829.

[25] Tjaderhane L, Nascimento FD, Breschi L. Optimizing dentin bond durability: control of collagen degradation by matrix metalloproteinases and cysteine cathepsins. Dent Mater, 2013, 29(1): 116-135.

[26] Niu LN, Zhang L, Jiao K. Localization of MMP-2, MMP-9, TIMP-1, and TIMP-2 in human coronal dentine. J Dent, 2011, 39(8): 536-542.

[27] Carrilho MRO, Geraldeli S, Tay F. In vivo preservation of the hybrid layer by chlorhexidine. J Dent Res, 2007, 86(6): 529-533.

[28] La VD, Bergeron C, Gafner S. Grape seed extract suppresses lipopolysaccharide-induced matrix metalloproteinase (MMP) secretion by macrophages and inhibits human MMP-1 and -9 activities. J Periodontol, 2009, 80(11): 1875-1882.

[29] Ebrahimi A, Schluesener H. Natural polyphenols against neurodegenerative disorders: potentials and pitfalls. Ageing Res Rev, 2012, 11(2): 329-345.

[30] Ku CS, Sathishkumar M, Mun SP. Binding affinity of proanthocyanidin from waste Pinus radiata bark onto proline-rich bovine achilles tendon collagen type I. Chemosphere, 2007, 67(8): 1618-1627.

[31] Sela-Passwell N, Rosenblum G, Shoham T. Structural and functional bases for allosteric control of MMP activities: can it pave the path for selective inhibition. Biochim Biophys Acta, 2010, 1803(1): 29-38.

[32] Feghali K, Feldman M, La VD. Cranberry proanthocyanidins: natural weapons against periodontal diseases. J Agric Food Chem, 2012, 60(23): 5728-5735.

[33] Nam K, Kimura T, Kishida A. Physical and biological properties of collagen-phospholipid polymer hybrid gels. Biomaterials, 2007, 28(20): 3153-3162.

[34] Cova A, Breschi L, Nato F. Effect of UVA-activated riboflavin on dentin bonding. J Dent Res, 2011, 90(12): 1439-1445.

[35] Macedo GV, Yamauchi M, Bedran-Russo AK. Effects of chemical cross-linkers on caries-affected dentin bonding. J Dent Res, 2009, 88(12): 1096-1100.

[36] Tay FR, Pashley DH. Biomimetic remineralization of resin-bonded acid-etched dentin. J Dent Res, 2009, 88(8): 719-724.

[37] Tay FR, Pashley DH. Guided tissue remineralisation of partially demineralised human dentine. Biomaterials, 2008, 29(8): 1127-1137.