Differentially expressed genes and signalling pathways are involved in mouse osteoblast-like MC3T3-E1 cells exposed to 17-β estradiol

Abstract

Bone-forming cells drastically alter their gene expression patterns in response to the hormone estrogen, according to researchers in China. A team led by Hui-Qiang Sun from Shandong University used microarray analyses to identify all the genes in a mouse bone-forming cell line (MC3T3-E1), where expression increased or decreased following treatment with a form of estrogen known as 17-β estradiol. Approximately 5 400 genes showed changed expression patterns. The researchers classified these genes into more than 1 500 different functional groups involved in approximately 50 different molecular pathways. In general, genes associated with cell proliferation and differentiation were switched on by estrogen, whereas genes involved in programmed cell death and bone resorption were switched off by the hormone treatment. The findings could guide therapies that aim to promote bone regeneration.

Keywords

17-β estradiol / MC3T3-E1 cell / microarray / signal transduction

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