Low-power laser irradiation promotes the proliferation and osteogenic differentiation of human periodontal ligament cells via cyclic adenosine monophosphate

Abstract

Exposure to low-power laser light could stimulate the repair of jaw tissues that have been damaged in the course of periodontal disease. Dentists have several strategies for addressing severe plaque build-up in the teeth and gums, but these are generally inadequate for repairing the resulting destruction of root, jaw and connective tissue. Inspired by research demonstrating that low-power laser irradiation (LPLI) may promote wound-healing, Yan-Hsiung Wang of Kaohsiung Medical University in Taiwan and colleagues investigated the potential benefits of LPLI in treating periodontal damage. They demonstrated that this treatment stimulated active proliferation and bone-producing activity in human periodontal ligament (hPDL) cells, which are primarily responsible for the formation and maintenance of periodontal tissue. These results indicate that LPLI could be a valuable tool for promoting tissue regeneration in patients.

Keywords

cell proliferation / cyclic adenosine monophosphate / human periodontal ligament cells / low-power laser irradiation / osteogenic differentiation

References

[1] Nanci A . Ten Cate’s oral histology: development, structure, and function. St Louis: Mosby, 2007.

[2] Rygh P. Ultrastructural changes in tension zones of rat molar periodontium incident to orthodontic tooth movement. Am J Orthod, 1976, 70(3): 269-281.

[3] Christgau M, Caffesse RG, Schmalz G. Extracellular matrix expression and periodontal wound-healing dynamics following guided tissue regeneration therapy in canine furcation defects. J Clin Periodontol, 2007, 34(8): 691-708.

[4] Niemiec BA. Periodontal therapy. Top Companion Anim Med, 2008, 23(2): 81-90.

[5] Gottlow J, Nyman S, Lindhe J. New attachment formation in the human periodontium by guided tissue regeneration. Case reports. J Clin Periodontol, 1986, 13(6): 604-616.

[6] Bowers GM, Chadroff B, Carnevale R. Histologic evaluation of new attachment apparatus formation in humans. Part I. J Periodontol, 1989, 60(12): 664-674.

[7] Bertolini GR, Artifon EL, Silva TS. Low-level laser therapy, at 830 nm, for pain reduction in experimental model of rats with sciatica. Arq Neuropsiquiatr, 2011, 69(2B): 356-359.

[8] Doshi-Mehta G, Bhad-Patil WA. Efficacy of low-intensity laser therapy in reducing treatment time and orthodontic pain: a clinical investigation. Am J Orthod Dentofacial Orthop, 2012, 141(3): 289-297.

[9] de Lima FM, Villaverde AB, Albertini R. Dual effect of low-level laser therapy (LLLT) on the acute lung inflammation induced by intestinal ischemia and reperfusion: action on anti- and pro-inflammatory cytokines. Lasers Surg Med, 2011, 43(5): 410-420.

[10] Pires D, Xavier M, Araujo T. Low-level laser therapy (LLLT; 780 nm) acts differently on mRNA expression of anti- and pro-inflammatory mediators in an experimental model of collagenase-induced tendinitis in rat. Lasers Med Sci, 2011, 26(1): 85-94.

[11] Goncalves RV, Novaes RD, do Carmo Cupertino M. Time-dependent effects of low-level laser therapy on the morphology and oxidative response in the skin wound healing in rats. Lasers Med Sci, 2012, 28(2): 383-390.

[12] Melo VA, Anjos DC, Albuquerque Junior R. Effect of low level laser on sutured wound healing in rats. Acta Cir Bras, 2011, 26(2): 129-134.

[13] Hou JF, Zhang H, Yuan X. In vitro effects of low-level laser irradiation for bone marrow mesenchymal stem cells: proliferation, growth factors secretion and myogenic differentiation. Lasers Surg Med, 2008, 40(10): 726-733.

[14] Soleimani M, Abbasnia E, Fathi M. The effects of low-level laser irradiation on differentiation and proliferation of human bone marrow mesenchymal stem cells into neurons and osteoblasts—an in vitro study. Lasers Med Sci, 2012, 27(2): 423-430.

[15] da Silva AP, Petri AD, Crippa GE. Effect of low-level laser therapy after rapid maxillary expansion on proliferation and differentiation of osteoblastic cells. Lasers Med Sci, 2012, 27(4): 777-783.

[16] Wu JY, Chen CH, Wang CZ. Low-power laser irradiation suppresses inflammatory response of human adipose-derived stem cells by modulating intracellular cyclic AMP level and NF-kappaB activity. PloS ONE, 2013, 8(1): e54067.

[17] Wang YH, Ho ML, Chang JK. Microporation is a valuable transfection method for gene expression in human adipose tissue-derived stem cells. Mol Ther, 2009, 17(2): 302-308.

[18] Silverio KG, Rodrigues TL, Coletta RD. Mesenchymal stem cell properties of periodontal ligament cells from deciduous and permanent teeth. J Periodontol, 2010, 81(8): 1207-1215.

[19] Seo BM, Miura M, Gronthos S. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet, 2004, 364(9429): 149-155.

[20] Isaka J, Ohazama A, Kobayashi M. Participation of periodontal ligament cells with regeneration of alveolar bone. J Periodontol, 2001, 72(3): 314-323.

[21] Hakki SS, Korkusuz P, Berk G. Comparison of Er,Cr:YSGG laser and hand instrumentation on the attachment of periodontal ligament fibroblasts to periodontally diseased root surfaces: an in vitro study. J Periodontol, 2010, 81(8): 1216-1225.

[22] Shimizu N, Yamaguchi M, Goseki T. Inhibition of prostaglandin E2 and interleukin 1-beta production by low-power laser irradiation in stretched human periodontal ligament cells. J Dent Res, 1995, 74(7): 1382-1388.

[23] Ozawa Y, Shimizu N, Abiko Y. Low-energy diode laser irradiation reduced plasminogen activator activity in human periodontal ligament cells. Lasers Surg Med, 1997, 21(5): 456-463.

[24] Yu Y, Mu J, Fan Z. Insulin-like growth factor 1 enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells via ERK and JNK MAPK pathways. Histochem Cell Biol, 2012, 137(4): 513-525.

[25] Lee JH, Um S, Jang JH. Effects of VEGF and FGF-2 on proliferation and differentiation of human periodontal ligament stem cells. Cell Tissue Res, 2012, 348(3): 475-484.

[26] Zhou Y, Wu C, Xiao Y. The stimulation of proliferation and differentiation of periodontal ligament cells by the ionic products from Ca₇Si₂P₂O₁₆ bioceramics. Acta Biomater, 2012, 8(6): 2307-2316.

[27] Xia L, Zhang Z, Chen L. Proliferation and osteogenic differentiation of human periodontal ligament cells on akermanite and beta-TCP bioceramics. Eur Cell Mater, 2011, 22: 68-82.

[28] Kreisler M, Christoffers AB, Willershausen B. Effect of low-level GaAlAs laser irradiation on the proliferation rate of human periodontal ligament fibroblasts: an in vitro study. J Clin Periodontol, 2003, 30(4): 353-358.

[29] Choi EJ, Yim JY, Koo KT. Biological effects of a semiconductor diode laser on human periodontal ligament fibroblasts. J Periodontal Implant Sci, 2010, 40(3): 105-110.

[30] Walsh LJ. The current status of low level laser therapy in dentistry. Part 1. Soft tissue applications. Aust Dent J, 1997, 42(4): 247-254.

[31] Gether U. Uncovering molecular mechanisms involved in activation of G protein-coupled receptors. Endocrine reviews, 2000, 21(1): 90-113.

[32] Zungu IL, Hawkins Evans D, Abrahamse H. Mitochondrial responses of normal and injured human skin fibroblasts following low level laser irradiation—an in vitro study. Photochem Photobiol, 2009, 85(4): 987-996.

[33] Karu TI, Lobko VV, Lukpanova GG. Effect of irradiation with monochromatic visible light on the cAMP content in mammalian cells. Dokl Akad Nauk SSSR, 1985, 281(5): 1242-1244.

[34] Doorn J, Leusink M, Groen N. Diverse Effects of cyclic AMP variants on osteogenic and adipogenic differentiation of human mesenchymal stromal cells. Tissue Eng Part A, 2012, 18(13/14): 1431-1442.

[35] Siddappa R, Doorn J, Liu J. Timing, rather than the concentration of cyclic AMP, correlates to osteogenic differentiation of human mesenchymal stem cells. J Tissue Eng Regen Med, 2010, 4(5): 356-365.

[36] James MA, Lu Y, Liu Y. RGS17, an overexpressed gene in human lung and prostate cancer, induces tumor cell proliferation through the cyclic AMP–PKA–CREB pathway. Cancer Res, 2009, 69(5): 2108-2116.

[37] Takahashi H, Honma M, Miyauchi Y. Cyclic AMP differentially regulates cell proliferation of normal human keratinocytes through ERK activation depending on the expression pattern of B-Raf. Arch Dermatol Res, 2004, 296(2): 74-82.

[38] Rocha AS, Paternot S, Coulonval K. Cyclic AMP inhibits the proliferation of thyroid carcinoma cell lines through regulation of CDK4 phosphorylation. Mol Biol Cell, 2008, 19(11): 4814-4825.

[39] Inada A, Hamamoto Y, Tsuura Y. Overexpression of inducible cyclic AMP early repressor inhibits transactivation of genes and cell proliferation in pancreatic beta cells. Mol Cell Biol, 2004, 24(7): 2831-2841.