A novel porcine acellular dermal matrix scaffold used in periodontal regeneration

Abstract

Grafting a new biologically derived matrix onto damaged dental tissue may help cell regeneration, research from China and the USA shows. Guo-qiang Guan at the State University of New York, USA, and co-workers at that institute and Shandong University, China, have successfully designed and trialed a matrix scaffold for use in the repair of periodontal tissues, which surround and support the teeth. The team removed cells from sections of pig tissue, while leaving the extracellular framework intact to create so-called ‘porcine acellular dermal matrix’ (PADM) grafts. They then treated the PADMs with hydroxyapatite (HA) — a mineral form of calcium apatite similar to that found in dental enamel and dentin. The HA-PADM provided an excellent, biodegradable scaffold for the regeneration of periodontal tissue cells both in laboratory cultures and in rabbits.

Keywords

periodontal ligament cells / periodontal regeneration / porcine acellular dermal matrix / scaffold / tissue engineering

References

[1] Schroder NW, Meister D, Wolff V. Chronic periodontal disease is associated with single nucleotide polymorphisms of the human TLR-4 gene. Genes Immun, 2005, 6(5): 448-451.

[2] Deepa D, Mehta DS, Puri VK. Combined periodontic-orthodontic-endodontic interdisciplinary approach in the treatment of periodontally compromised tooth. J Indian Soc Periodontol, 2010, 14(2): 139-143.

[3] Karring T, Nyman S, Gottlow J. Development of the biological concept of guided tissue regeneration—animal and human studies. Periodontol 2000, 1993, 1(1): 26-35.

[4] Cho EH, Park JC, Cha JK. Dimensional change of the healed periosteum on surgically created defects. J Periodontal Implant Sci, 2011, 41(4): 176-184.

[5] Giannopoulou C, Cimasoni G. Functional characteristics of gingival and periodontal ligament fibroblasts. J Dent Res, 1996, 75(3): 895-902.

[6] Wang LH, Greenwell H, Fiorellini J. Periodontal regeneration. J Periodontol, 2005, 76(9): 1601-1622.

[7] Chen FM, Zhao YM, Zhang R. Periodontal regeneration using novel glycidyl methacrylated dextran (Dex-GMA)/gelatin scaffolds containing microspheres loaded with bone morphogenetic proteins. J Controlled Release, 2007, 121(1/2): 81-90.

[8] Yang ZH, Jin F, Zhang XJ. A novel possible strategy based on self-assembly approach to achieve complete periodontal regeneration. J Artificial Organs, 2010, 34(7): 603-609.

[9] Seo BM, Miura M, Gronthos S. Investigation of multipotent postnatal stem cells from human periodontal ligament. J Lancet, 2004, 364(9429): 149-155.

[10] Lin CC, Ritch R, Lin SM. A new fish scale-derived scaffold for corneal regeneration. J Eur Cells Mater, 2010, 19: 50-57.

[11] Lee CH, Singla A, Lee Y. Biomedical applications of collagen. Int J Pharm, 2001, 221(1/2): 1-22.

[12] Ma L, Gao C, Mao Z. Enhanced biological stability of collagen porous scaffolds by using amino acids as novel cross-linking bridges. Biomaterials, 2004, 25(15): 2997-3004.

[13] Hoerstrup SP, Sodian R, Daebritz S. Functional living trileaflet heart valves grown in vitro. . Circulation, 2000, 102(19 Suppl 3): III44-III49.

[14] Shinoka T, Shum-Tim D, Ma PX. Creation of viable pulmonary artery autografts through tissue engineering. J Thorac Cardiovasc Surg, 1998, 115(3): 536-546.

[15] Stock UA, Nagashima M, Khalil PN. Tissue-engineeredvalved conduits in the pulmonary irculation. J Thorac Cardiovasc Surg, 2000, 119(4): 732-740.

[16] Steinhoff G, Stock U, Karim N. Tissue engineering of pulmonary heart valves on allogenic acellular matrix conduits: in vivo restoration of valve tissue. Circulation, 2000, 102(19 Suppl 3): III50-III55.

[17] Joshi P, Chung C, Aukhil I. Endothelial cells adhere to the RGD domain and the fibrinogen-like terminal knob of tenascin. J Cell Sci, 1993, 106(Pt 1): 389-400.

[18] Ngangan AV, McDevitt TC. Acellularization of embryoid bodies via physical disruption methods. Biomaterials, 2009, 30(6): 1143-1149.

[19] Niederkorn JY. Immune mechanisms of corneal allograft rejection. Curr Eye Res, 2007, 32(12): 1005-1016.