Chemical composition of Galla chinensis extract and the effect of its main component(s) on the prevention of enamel demineralization in vitro

Abstract

Gallachinensis extract (GCE), the major component of a traditional Chinese medicine, can protect teeth against caries, scientists show. For thousands of years, Gallachinensis has found use in traditional medicines ranging from antibacterial to anticancer agents. From a chemical analysis, a team led by Xue-Dong Zhou at Sichuan University in China and Jacob ten Cate at the University of Amsterdam in the Netherlands found that gallic acid accounts for 71% of GCE's major organic components. Through washing bovine teeth with either GCEor gallic acid, followed by an acid buffer,the researchers showed that these natural compounds are almost equally effective at inhibiting demineralization of tooth enamel. The author's suggest that gallic acid is the herb's anti-cavity agent, so could be added to food and drink to improve a population's dental health.

Keywords

active component / Galla chinensis / gallic acid / composition / dental caries

References

[1] Tian F, Li B, Ji B. Identification and structure-activity relationship of gallotannins separated from Galla chinensis. . LWT-Food Sci Technol, 2009, 42(7): 1289-1295.

[2] Djakpo O, Yao W. Rhus chinensis and Galla chinensis—folklore to modern evidence: review. Phytother Res, 2010, 24(12): 1739-1747.

[3] Cheng L, Exterkate RA, Zhou X. Effect of Galla chinensis on growth and metabolism of microcosm biofilms. Caries Res, 2011, 45(2): 87-92.

[4] Cheng L, Li JY, Hao YQ. Effect of compounds of Galla chinensis and their combined effects with fluoride on remineralization of initial enamel lesion in vitro. . J Dent, 2008, 36(5): 369-373.

[5] Chu JP, Li JY, Hao YQ. Effect of compounds of Galla chinensis on remineralisation of initial enamel carious lesions in vitro. . J Dent, 2007, 35(5): 383-387.

[6] Xie Q, Li J, Zhou X. Anticaries effect of compounds extracted from Galla Chinensis in a multispecies biofilm model. Oral Microbiol Immunol, 2008, 23(6): 459-465.

[7] Huang S, Gao S, Cheng L. Combined effects of nano-hydroxyapatite and Galla chinensis on remineralisation of initial enamel lesion in vitro. . J Dent, 2010, 38(10): 811-819.

[8] Zou L, Zhang LL, Li JY. Effect of Galla chinensis extract and chemical fractions on demineralization of bovine enamel in vitro. . J Dent, 2008, 36(12): 999-1004.

[9] Zhang LL, Xue J, Li JY. Effects of Galla chinensis on inhibition of demineralization of regular bovine enamel or enamel disposed of organic matrix. Arch Oral Biol, 2009, 54(9): 817-822.

[10] Cheng L, ten Cate JM. Effect of Galla chinensis on the in vitro remineralization of advanced enamel lesions. Int J Oral Sci, 2010, 2(1): 15-20.

[11] Shi B, Di Y. Plant polyphenol, 2000 Beijing 30-31.

[12] Jiang Y, David B, Tu P. Recent analytical approaches in quality control of traditional Chinese medicines—a review. Anal Chim Acta, 2010, 657(1): 9-18.

[13] Ball P. History of science: quinine steps back in time. Nature, 2008, 451(7182): 1065-1066.

[14] White NJ. Qinghaosu (Artemisinin): the price of success. Science, 2008, 320(5874): 330-334.

[15] ten Cate JM, Buijs MJ, Miller CC. Elevated fluoride products enhance remineralization of advanced enamel lesions. J Dent Res, 2008, 87(10): 943-947.

[16] ten Cate JM, Duijsters PP. Influence of fluoride in solution on tooth demineralization. Caries Res, 1983, 17(6): 193-199.

[17] Phan TN, Buckner T, Sheng J. Physiologic actions of zinc related to inhibition of acid and alkali production by oral streptococci in suspensions and biofilms. Oral Microbiol Immunol, 2004, 19(1): 31-38.

[18] Snyder LR, Dolan JW . High-performance gradient elution: the practical application of the linear-solvent-strength model. Hoboken: Wiley Interscience, 2007.

[19] Iswaldi I, Arráez-Román D, Rodríguez-Medina I. Identification of phenolic compounds in aqueous and ethanolic rooibos extracts (Aspalathus linearis) by HPLC-ESI-MS (TOF/IT). Anal Bioanal Chem, 2011, 400(10): 3643-3654.

[20] Tian F, Li B, Ji B. Antioxidant and antimicrobial activities of consecutive extracts from Galla chinensis: the polarity affects the bioactivities. Food Chem, 2009, 113(1): 173-179.

[21] Xiang P, Lin Y, Lin P. Effect of cationization reagents on the matrix-assisted laser desorption/ionization time-of-flight mass spectrum of Chinese gallotannins. J Appl Polym Sci, 2007, 105(2): 859-864.

[22] Travis DF, Glimcher MJ. The structure and organization of, and the relationship between the organic matrix and the inorgainc crystals of embrayonic bovine enamel. J Cell Biol, 1964, 23(3): 447-497.

[23] Yu S. Oral histology and pathology, 2007 Beijing 47-48.

[24] Featherstone JD, Rosenberg H. Lipid effect on the progress of artificial carious lesions in dental enamel. Caries Res, 1984, 18(1): 52-55.

[25] Fang Z, Zhang M, Tao G. Chemical composition of clarified bayberry (Myrica rubra Sieb. et Zucc.) juice sediment. J Agric Food Chem, 2006, 54(20): 7710-7716.

[26] Kang MS, Oh JS, Kang IC. Inhibitory effect of methyl gallate and gallic acid on oral bacteria. J Microbiol, 2008, 46(6): 744-750.

[27] Hsu CL, Yen GC. Effect of gallic acid on high fat diet-induced dyslipidaemia, hepatosteatosis and oxidative stress in rats. Br J Nutr, 2007, 98(4): 727-735.

[28] Q He, B Shi, K Yao. Synthesis of 6-O-galloyl-d-glucose, 3,6-di-O-galloyl-d-glucose and 3,4,6-tri-O-galloyl-d-glucose. J Sichuan Univ (Eng Sci Edition), 2002, 34(3): 110-113.

[29] Q He, B Shi, K Yao. Interactions of gallotannins with proteins, amino acids, phospholipids and sugars. Food Chem, 2006, 95(2): 250-254.