Laser capture microdissection enables cellular and molecular studies of tooth root development

Abstract

Meticulous laser excision of oral tissues has enabled researchers from the US and China to identify genes involved in tooth-root development. Better understanding of the spatial and temporal regulation of these genes could open the way for gum and tooth regenerative therapies. Led by Hai Zhang from the University of Washington,Seattle,the researchers used two complementary gene profiling approaches to identify genes that were differentially expressed in pure samples of five cell- and tissue-types excised from one- and two-week-old mice using the technique known as laser capture microdissection. The findings will help scientists to elucidate the carefully orchestrated molecular interactions between the epidermal and mesenchymal cell layers that control root formation,including the dramatic differentiation of soft tissues into enamel—the body's hardest tissue.

Keywords

gene / laser capture microdissection / microarray / PCR / root

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