Opposing regulation of L-theanine biosynthesis by CsWRKY65 and CsWRKY69 in tea plant roots

Peiying Liu , Yongheng Zhang , Yumeng Bao , Dan Chen , Yezi Xiao , Hongyan Deng , Ziyao Ge , Pengjie Wang , Youben Yu

Horticulture Research ›› 2025, Vol. 12 ›› Issue (8) : 131

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Horticulture Research ›› 2025, Vol. 12 ›› Issue (8) :131 DOI: 10.1093/hr/uhaf131
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Opposing regulation of L-theanine biosynthesis by CsWRKY65 and CsWRKY69 in tea plant roots
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Peiying Liu, Yongheng Zhang, Yumeng Bao, Dan Chen, Yezi Xiao, Hongyan Deng, Ziyao Ge, Pengjie Wang, Youben Yu. Opposing regulation of L-theanine biosynthesis by CsWRKY65 and CsWRKY69 in tea plant roots. Horticulture Research, 2025, 12(8): 131 DOI:10.1093/hr/uhaf131

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Acknowledgements

This work was supported by the National key research and development program (2022YFD1602003), China Agriculture Research System of MOF and MARA (CARS-19), the Agricultural Special Fund Project of Shaanxi Province (2024NYGG009), and the special fund for University-Supported Extension Model (XTG2025-04).

Author contributions

P.L. and Y.Z. conceived and designed the experiments. P.L., Y.Z., and D.C. performed the experiments and data analysis. P.L. and Y.Z. wrote the manuscript. Y.B., Z.G., H.D., and Y.X. provided valuable advice on the manuscript. Y.Y. and P.W. revised the manuscript. All authors contributed to the article and approved the submitted version.

Data availability

The data that support the findings of this study are publicly available in the Tea Plant Information Archive (TPIA): a comprehensive knowledge database for tea plant (http://tpia.teaplants.cn/index.html).

Conflict of interest statement

The authors declare that they have no conflict of interest.

References

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Li M, Liu H, Wu D. et al. l-Theanine: a unique functional amino acid in tea (Camellia sinensis L.) with multiple health benefits and food applications. Front Nutr. 2022; 9:853846

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Zhu B, Guo J, Dong C. et al. CsAlaDC and CsTSI work coordinately to determine theanine biosynthesis in tea plants (Camellia sinensis L.) and confer high levels of theanine accumulation in a non-tea plant. Plant Biotechnol J. 2021; 19:2395-7

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Guo J, Zhu B, Chen Y. et al. Potential ‘accelerator’ and ‘brake’ regulation of theanine biosynthesis in tea plant. Hortic Res. 2022;9:uhac169

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Gao Y, Sun C, Zhang X. et al. Identification of the GATA tran-scription factor family in tea plant (Camellia sinensis) and the characterizations in nitrogen metabolism. Plant Physiol Biochem. 2025; 221:109661

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Ma J, Liu N, Sun X. et al. Establishment of an efficient transforma-tion system and its application in regulatory mechanism analysis of biological macromolecules in tea plants. Int J Biol Macromol. 2023; 244:125372

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