Developing BrapaCapture40K liquid chip for genetic research and breeding in Brassica rapa

Yifan Zhou; Liping Song; Lijie Zhong; Liguang Tang; Xueqing Zhou; Yuxian Zhu; Kun Wang; Aihua Wang

doi:10.1093/hr/uhaf123

Horticulture Research ›› 2025, Vol. 12 ›› Issue (8) :123 DOI: 10.1093/hr/uhaf123

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Developing BrapaCapture40K liquid chip for genetic research and breeding in Brassica rapa

Yifan Zhou ¹^,²^,^‡
, Liping Song ¹^,^‡
, Lijie Zhong ²
, Liguang Tang ¹
, Xueqing Zhou ¹
, Yuxian Zhu ²^,³^,⁴
, Kun Wang ¹^,²^,⁴^,^*
, Aihua Wang ¹^,^*

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Yifan Zhou, Liping Song, Lijie Zhong, Liguang Tang, Xueqing Zhou, Yuxian Zhu, Kun Wang, Aihua Wang. Developing BrapaCapture40K liquid chip for genetic research and breeding in Brassica rapa. Horticulture Research, 2025, 12(8): 123 DOI:10.1093/hr/uhaf123

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Acknowledgements

We thank the China Agriculture Research System of MOF and MARA (CARS-23-G27 and HBZY2023A001-02), Wuhan Major Project of Key Technologies in Biological Breeding and New Variety Cultivation (2022021302024852), Qinghai Province Major Science and Technology Special - Rural Department (2024-0101-NCC-0001), China Agriculture Research System of MOF and MARA (CARS-23-G27), Hubei Province Seed Industry High-quality Development Fund Project (HBZY2023B004-4), Wuhan Knowledge Innovation Special Dawn Project (2023020201020468), Xining City Science and Technology Plan Project (2023-Y-17), Hubei Provincial Key R&D Program (2021BBA102) and Hubei Provincial Natural Science Foundation of China (2021CFA055 and 2021BBA102) for the financial support.

Author contributions

A.W. conceived the project and was responsible for the initiation of the project. L.S., L.T., and X.Z. supervised and performed the chip design, sample preparation, and sequencing. Z.Y. and L.Z. collected the data, performed the bioinformatics analysis, and wrote the paper. Y.Z., K.W., and A.W. contributed to revisions.

Data availability

All the raw sequencing data related are archived at Genome Sequence Archive of China National Center for Bioinformation (https://www.cncb.ac.cn/) under the project PRJCA034964. The detailed information (Supplemental Table) of BrapaCapture40K can be downloaded at the OMIX database (https://ngdc.cncb.ac.cn/omix/) under the accession OMIX008727.

Conflict of interest statement

The authors declare no competing interests.

References

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[2]	Thomas W-J-W, Zhang YQ, Amas J-C. et al. Innovative advances in plant genotyping. In: Shavrukov Y,ed. Plant Genotyping:Methods and Protocols. Springer US: New York, NY, 2023,451-65

[3]	Cheng F, Sun RF, Hou XL. et al. Subgenome parallel selection is associated with morphotype diversification and convergent crop domestication in Brassica rapa and Brassica oleracea. Nat Genet. 2024; 48:1218-24

[4]	Zhou YF, Ye HZ, Liu EW. et al. The complexity of structural variations in Brassica rapa revealed by assembly of two complete T2T genomes. Sci Bull. 2024; 69:2346-51