Wo interacts with SlTCP25 to regulate type I trichome branching in tomato

Abstract

Plant trichomes serve as a protective barrier against various stresses. Although the molecular mechanisms governing the initiation of trichomes have been extensively studied, the regulatory pathways underlying the trichome branching in tomato remain elusive. Here, we found that Woolly (Wo) mutant and its overexpression transgenic plants displayed branched type I trichomes. The expression level of SlTCP25, a transcription factor of type TB1 of the TCP subfamily, was obviously decreased in Wo mutant and Wo overexpressing lines. Knockout of SlTCP25 resulted in the formation of type I trichome branches on the hypocotyls. Genetic evidence showed that SlTCP25 is epistatic to Wo in the branched trichome formation. Biochemical data further indicated that Wo can directly bind to the L1-box cis-element in the SlTCP25 promoter and repress its transcription. We further determined that SlTCP25 interacts with Wo to weaken Wo-regulated the expression of SlCycB2, a trichome branching inhibitor. In addition, the number of trichome branches was significantly increased in Sltcp25Slcycb2 double mutant, suggesting that SlTCP25 and SlCycB2 coordinately repress trichome branching in wild type. In conclusion, we elucidate a molecular network governing the morphogenesis of multicellular trichomes in tomato.

Acknowledgements

This work was supported by grants from the National Key Research and Development Program of China(2021YFF1000104).

Author contributions

CY conceived and supervised the research. JW, SY, YZ, XS, and LW performed experiments. JW analyzed the data and wrote the manuscript. CY and TW modified the manuscript.

Data availability

The data that supports the findings of this study are available in the supplemental information of this article.

Conflict of interest statement

None declared.

Supplementary Data

Supplementary data are available at Horticulture Research online.

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