Spatiotemporally transcriptomic analyses of floral buds reveal the high-resolution landscape of flower development and dormancy regulation in peach

Abstract

The spatiotemporal transcriptome dataset reported here provides the peach flower bud’s gene expression atlas at spatiotemporal resolution level using the 10x Genomics Visium platform. This dataset can be used to define transcript accumulation for any interesting genes across several flower bud cells. It was generated using three peach flower bud samples during the activity-dormancy period, providing valuable insight into gene expression profiling and developmental stages under different environmental contexts or conditions. Importantly, we found that different cell types are related to specific regulatory programs, including signal transduction, environment and stress responses, and flower development. Our research provides insight into the transcriptomic landscape of the key cell types for flower buds and opens new avenues to study cell-type specification, function, and differentiation in Rosaceae fruit trees. A series of pivotal genes (e.g. AMS, MS188, MS1) for flower bud development were identified. These results provide a valuable reference for the activity-dormancy transition in perennial deciduous fruit trees.

Acknowledgements

Thanks to the National Horticultural Germplasm Resources Center and the National Peach germplasm Resource Nursery. This work was supported by the National Key Research and Development Program (2023YFE0105400, 2019YFD1000200), the National Natural Science Foundation of China (32341042), the Central Public-interest Scientific Institution Basal Research Fund (Y2022QC23, 1610192023310), the Agricultural Science and Technology Innovation Program (CAAS-ASTIP-2024-ZFRI-01), the Natural Science Foundation of Henan (232300421042), the National Science and Technology Major Project of Yunan (202302AE090005-3), and the Crop Germplasm Resources Conservation Project (2016NWB041). We would like to thank Abebe Misganaw for his help in revising the article.

Author contributions

L.W. and Y.L. planned and designed the research. Y.Z., X.C., and D.G. performed the experiments. W.F., C.C., X.W., J.W., and K.C. conducted fieldwork. Y.Z. managed the experimental materials. Y.Z. wrote the manuscript and analyzed the sequencing data.

Data availability statement

All relevant data are available within the article and the supplementary data.

Conflict of interests

The authors have no conflict of interest that would bias the collection, analysis, reporting, or publishing of the research in the manuscript.

Supplementary Data

Supplementary data is available at Horticulture Research online.

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