A multiplex microfluidic device to detect miRNAs for diagnosis of plant growth status

Acknowledgements

We thank M. Taniguchi for technical assistance. Funding: This work was supported by grants from the Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (JP21H05657 to M.N. and JP22H04536 to M.H.), the Japan Science and Technology Agency (PRESTO 15665754, CREST JPMJCR15O2, SCORE 2110336, START 2210365 to M.N.), and the NARO Bio-oriented Technology Research Advancement Institution (SBIR 21488775 to M.N.).

Author contributions

M.N. conceived this study. Y.K., R.O., and M.H. designed and conducted the main experiments with advices from A.A., Y.B., K.K., and M.N. Y.K. and M.N. wrote the paper.

Data availability

Data generated or analyzed during this study are included in this article.

Conflict of interest statement

Nagoya University has filed for patents regarding the following topics: ‘Fluidic chip for plant substances detection,’ inventors M.N., R.O., and Naoki Yanagisawa (patent publication nos. JP2018-111362); ‘Fluidic chip for plant substance detection and plant substance detection equipment,’ inventors M.N. and Y.K. (patent application nos. JP 2019-190 996); ‘Highly sensitive diagnostic device to detect biomolecules in plants,’ inventors M.N., M.H., Y.K., and A.A. (patent application nos. JP 2023-047707).

Supplementary data

Supplementary data is available at Horticulture Research online.

References

[1] Zeng H, Wang G, Hu X. et al. Role of microRNAs in plant responses to nutrient stress. Plant and Soil. 2014;374: 1005-21

[2] Chiou T-J, Aung K, Lin S-I. et al. Regulation of phosphate homeostasis by microRNA in Arabidopsis. Plant Cell. 2006;18: 412-21

[3] Kawakatsu Y, Okada R, Hara M. et al. Microfluidic device for simple diagnosis of plant growth condition by detecting miRNAs from filtered plant extracts. Plant Phenomics. 2024;6:0162

[4] Arata H, Komatsu H, Hosokawa K. et al. Rapid and sensi-tive microRNA detection with laminar flow-assisted dendritic amplification on power-free microfluidic Chip. PLoS One. 2012;7: e48329

[5] Sato K, Hamada M, Asai K. et al. CentroidFold: a web server for RNA secondary structure prediction. Nucleic Acids Res. 2009;37: W277-80

[6] Makoto K, Mari K, Yasuyuki N. et al. DeLTa-Seq: direct-lysate targeted RNA-Seq from crude tissue lysate. Plant Methods. 2022; 18:99

[7] Buhtz A, Pieritz J, Springer F. et al. Phloem small RNAs, nutrient stress responses, and systemic mobility. BMC Plant Biol. 2010;10:64

[8] Hsieh L-C, Lin S-I, Shih AC-C. et al. Uncovering small RNA-mediated responses to phosphate deficiency in Arabidopsis by deep sequencing. Plant Physiol. 2009;151: 2120-32

[9] Brás EJS, Margarida Fortes A, Chu V. et al. Microfluidic device for the point of need detection of a pathogen infection biomarker in grapes. Analyst. 2019;144: 4871-9