Two novel alleles of the MYB transcription factor BjA06.GL1 and BjB02.GL1 control leaf trichomes and enhance resistance to aphids in Brassica juncea

Abstract

Leaf trichome formation is a very important agronomic trait as it confers resistance to biotic and abiotic stresses, but the causal genes involved in this process in Brassica juncea remain largely unexplored. In this study, we first characterized the haplotypes of BjB02.GL1 among different inbred lines with leaf trichomes or glabrous leaves. A comparative analysis of the number and density of leaf trichomes between the two mustard inbred lines was then performed. BSA analysis of leaves with trichomes and glabrous pools from the F2 segregating population mapped the candidate genes on Chr.A06 and Chr.B02. Two candidate genes, BjA06.GL1 and BjB02.GL1, were subsequently cloned. After sequence alignment of the BjGL1 genes, both single-nucleotide polymorphisms (SNPs) and indel were identified in the BjA06.GL1 and BjB02.GL1 genes. And quantitative real-time polymerase chain reaction (qRT-PCR) analysis further confirmed that both the BjA06.GL1 and BjB02.GL1 genes were more highly expressed in leaves with trichomes than in glabrous leaves. As the leaf size increased, the leaf trichome density decreased. Gene editing of both BjA06.GL1 and BjB02.GL1 changed the leaf trichome to a glabrous leaf phenotype in mustard. In addition, plants with leaf trichomes presented greater resistance to aphids. Taken together, our results revealed that both BjA06.GL1 and BjB02.GL1 positively regulate leaf trichome formation and help increase aphid resistance in mustard. This study provides valuable resources and helps to elucidate the molecular mechanism of leaf trichome formation in B. juncea.

Acknowledgements

This research was supported by grants from the National Natural Science Foundation of China (32002056), the training program for young backbone teachers in Henan Province (2024GGJS085), the Natural Science Foundation of Henan Province (232300420021), and the Nanhu Scholars Program for Young Scholars of XYNU. We are grateful to Prof. Mingfang Zhang for kindly providing mustard seeds. The authors are grateful to the anonymous reviewers for their constructive comments for revisions.

Author contributions

H.S.P., Z.W., M.G.Z., X.F., W.Z.J., W.J., S.J.X., and F.T.D. conceived and designed all of the experiments. L.X.L., L.M., J.L.L., and L.M. performed the experiments. H.S.P. and L.X.L. analyzed the data. H.S.P. wrote the manuscript. All of the authors have read and approved the final manuscript.

Data availability

The data used in this study are available in the Genome Sequence Archive of the National Genomics Data Center and can be accessed with CRA017217.

Conflict of interest statement

The authors declare that they have no competing interests.

Supplementary Data

Supplementary data is available at Horticulture Research online.

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