Background: Surface-soil organic matter accumulation is expected under well-managed grasslands. Characterizing particulate and nonparticulate organic C and N fractions across a soil texture gradient under different conservation land management systems would help understand how grassland management can optimize soil organic matter to meet the challenges of environmental stress.
Methods: A cross-sectional study of 648 soil profiles sampled to 60-cm depth was conducted under a diversity of grasslands and woodlands in North Carolina, USA. Root-zone enrichments of particulate organic C and N were calculated as the difference between total and baseline stocks, derived from nonlinear depth distributions of C and N.
Results: Particulate organic C and N declined dramatically with depth, representing 35% ± 8% of total organic C at 0–10-cm depth, 14% ± 7% at 10–30-cm depth, and 10% ± 6% at 30–60-cm depth. Sand concentration had a significant negative association with both fractions. Root-zone enrichment of particulate organic C was lower under grassland than under woodland (14.2 vs. 16.7 Mg C ha−1, respectively, p < 0.001), while root-zone enrichment of particulate organic N was greater under grassland than under woodland (0.96 vs. 0.77 Mg N ha−1, respectively, p < 0.001). Root-zone enrichment of particulate organic C and N was increased by 17%–32% with poultry litter amendment and by 4%–20% in 30-year-old pastures compared with newer pastures.
Conclusions: Land use and management during the past few decades had a large influence on particulate organic C and N fractions, while pedogenic processes over millennia dominated the nonparticulate organic C and N stocks.
| [1] |
Beillouin, D., Cardinael, R., Berre, D., Boyer, A., Corbeels, M., Fallot, A., Feder, F., & Demenois, J. (2022). A global overview of studies about land management, land-use change, and climate change effects on soil organic carbon. Global Change Biology, 28(4), 1690–1702. https://doi.org/10.1111/gcb.15998
|
| [2] |
Bilgili, A., & Çomakli, E. (2021). Evaluation of the relationship between root nutrients and root biomass in lands under different management practices. Environmental Monitoring and Assessment, 193, 799. https://doi.org/10.1007/s10661-021-09585-y
|
| [3] |
Cambardella, C. A., & Elliott, E. T. (1992). Particulate soil organic matter changes across a grassland cultivation sequence. Soil Science Society of America Journal, 56(3), 777–783. https://doi.org/10.2136/sssaj1992.03615995005600030017x
|
| [4] |
Chomel, M., Lavallee, J. M., Alvarez-Segura, N., Baggs, E. M., Caruso, T., de Castro, F., Emmerson, M. C., Magilton, M., Rhymes, J. M., de Vries, F. T., Johnson, D., & Bardgett, R. D. (2022). Intensive grassland management disrupts below-ground multi-trophic resource transfer in response to drought. Nature Communications, 13(1), 6991. https://doi.org/10.1038/s41467-022-34449-5
|
| [5] |
Cotrufo, M. F., Ranalli, M. G., Haddix, M. L., Six, J., & Lugato, E. (2019). Soil carbon storage informed by particulate and mineral-associated organic matter. Nature Geoscience, 12(12), 989–994. https://doi.org/10.1038/s41561-019-0484-6
|
| [6] |
Don, A., Schumacher, J., & Freibauer, A. (2011). Impact of tropical land-use change on soil organic carbon stocks—A meta-analysis. Global Change Biology, 17(4), 1658–1670. https://doi.org/10.1111/j.1365-2486.2010.02336.x
|
| [7] |
Franzluebbers, A. J. (2010). Achieving soil organic carbon sequestration with conservation agricultural systems in the southeastern United States. Soil Science Society of America Journal, 74(2), 347–357. https://doi.org/10.2136/sssaj2009.0079
|
| [8] |
Franzluebbers, A. J. (2021). Soil organic carbon sequestration calculated from depth distribution. Soil Science Society of America Journal, 85(1), 158–171. https://doi.org/10.1002/saj2.20176
|
| [9] |
Franzluebbers, A. J. (2022a). Probing deep to express root-zone enrichment of soil-test biological activity on southeastern U.S. farms. Agricultural & Environmental Letters, 7(2), e20087. https://doi.org/10.1002/ael2.20087
|
| [10] |
Franzluebbers, A. J. (2022b). Root-zone soil organic carbon enrichment is sensitive to land management across soil types and regions. Soil Science Society of America Journal, 86(1), 79–91. https://doi.org/10.1002/saj2.20346
|
| [11] |
Franzluebbers, A. J. (2023a). Root-zone enrichment of soil-test biological activity and particulate organic carbon and nitrogen under conventional and conservation land management. Soil Science Society of America Journal, 87(6), 1431–1443. https://doi.org/10.1002/saj2.20574
|
| [12] |
Franzluebbers, A. J. (2023b). Soil organic carbon and nitrogen storage estimated with the root-zone enrichment method under conventional and conservation land management across North Carolina. Journal of Soil and Water Conservation, 78(2), 124–140. https://doi.org/10.2489/jswc.2023.00064
|
| [13] |
Franzluebbers, A. J. (2024a). Particulate organic carbon and nitrogen and soil-test biological activity under grazed pastures and conservation land uses. Soil Science Society of America Journal, 88(5), 1852–1869. https://doi.org/10.1002/saj2.20742
|
| [14] |
Franzluebbers, A. J. (2024b). Root-zone enrichment of soil organic carbon and nitrogen under grazing and other land uses in a humid-temperate region. Grass and Forage Science, 79(2), 265–280. https://doi.org/10.1111/gfs.12665
|
| [15] |
Franzluebbers, A. J. (2025a). Cumulative frequency distributions of soil health properties under grasslands and woodlands across North Carolina. Soil Science Society of America Journal, 89(5), e70142. https://doi.org/10.1002/saj2.70142
|
| [16] |
Franzluebbers, A. J. (2025b). Sand is the unifying textural component influencing surface-soil carbon and nitrogen fractions across undisturbed land uses in North Carolina. Soil Science Society of America Journal, 89(1), e70011. https://doi.org/10.1002/saj2.70011
|
| [17] |
Franzluebbers, A. J., & Stuedemann, J. A. (2002). Particulate and non-particulate fractions of soil organic carbon under pastures in the Southern Piedmont USA. Environmental Pollution, 116(S1), S53–S62. https://doi.org/10.1016/S0269-7491(01)00247-0
|
| [18] |
Gmach, M.-R., Dias, B. O., Silva, C. A., Nóbrega, J. C. A., Lustosa-Filho, J. F., & Siqueira-Neto, M. (2018). Soil organic matter dynamics and land-use change on Oxisols in the Cerrado, Brazil. Geoderma Regional, 14, e00178. https://doi.org/10.1016/j.geodrs.2018.e00178
|
| [19] |
Grandy, A. S., Strickland, M. S., Lauber, C. L., Bradford, M. A., & Fierer, N. (2009). The influence of microbial communities, management, and soil texture on soil organic matter chemistry. Geoderma, 150(3–4), 278–286. https://doi.org/10.1016/j.geoderma.2009.02.007
|
| [20] |
Jobbágy, E. G., & Jackson, R. B. (2000). The vertical distribution of soil organic carbon and its relation to climate and vegetation. Ecological Applications, 10(2), 423–436. https://doi.org/10.1890/1051-0761(2000)010[0423:TVDOSO]2.0.CO;2
|
| [21] |
Kingery, W. L., Wood, C. W., Delaney, D. P., Williams, J. C., Mullins, G. L., & van Santen, E. (1993). Implications of long-term land application of poultry litter on tall fescue pastures. Journal of Production Agriculture, 6(3), 390–395. https://doi.org/10.2134/jpa1993.0390
|
| [22] |
Kögel-Knabner, I., Guggenberger, G., Kleber, M., Kandeler, E., Kalbitz, K., Scheu, S., Eusterhues, K., & Leinweber, P. (2008). Organo-mineral associations in temperate soils: Integrating biology, mineralogy, and organic matter chemistry. Journal of Plant Nutrition and Soil Science, 171(1), 61–82. https://doi.org/10.1002/jpln.200700048
|
| [23] |
Li, X., Yang, T., Hicks, L. C., Hu, B., Li, F., Liu, X., Wei, D., Wang, Z., & Bao, W. (2023). Latitudinal patterns of particulate and mineral-associated organic matter down the soil profile in drylands. Soil and Tillage Research, 226, 105580. https://doi.org/10.1016/j.still.2022.105580
|
| [24] |
Loss, A., Pereira, M. G., Perin, A., Coutinho, F. S., & Cunha dos Anjos, L. H. (2013). Particulate organic matter in soil under different management systems in the Brazilian Cerrado. Soil Research, 50(8), 685–693. https://doi.org/10.1071/SR12196
|
| [25] |
Lugato, E., Lavallee, J. M., Haddix, M. L., Panagos, P., & Cotrufo, M. F. (2021). Different climate sensitivity of particulate and mineral-associated soil organic matter. Nature Geoscience, 14(5), 295–300. https://doi.org/10.1038/s41561-021-00744-x
|
| [26] |
Luo, Z., Viscarra-Rossel, R. A., & Qian, T. (2021). Similar importance of edaphic and climatic factors for controlling soil organic carbon stocks of the world. Biogeosciences, 18(6), 2063–2073. https://doi.org/10.5194/bg-18-2063-2021
|
| [27] |
Macinnis-Ng, C. M. O., Fuentes, S., O'Grady, A. P., Palmer, A. R., Taylor, D., Whitley, R. J., Yunusa, I., Zeppel, M. J. B., & Eamus, D. (2010). Root biomass distribution and soil properties of an open woodland on a duplex soil. Plant and Soil, 327(1–2), 377–388. https://doi.org/10.1007/s11104-009-0061-7
|
| [28] |
Mishra, U., Torn, M. S., Masanet, E., & Ogle, S. M. (2012). Improving regional soil carbon inventories: Combining the IPCC carbon inventory method with regression kriging. Geoderma, 189–190, 288–295. https://doi.org/10.1016/j.geoderma.2012.06.022
|
| [29] |
National Oceanic and Atmospheric Administration (NOAA). (2024). U.S. climate normals 2020: U.S. annual/seasonal climate normals (1991–2020). National Centers for Environmental Information. https://www.ncei.noaa.gov/
|
| [30] |
Ontl, T. A., Cambardella, C. A., Schulte, L. A., & Kolka, R. K. (2015). Factors influencing soil aggregation and particulate organic matter responses to bioenergy crops across a topographic gradient. Geoderma, 255–256, 1–11. https://doi.org/10.1016/j.geoderma.2015.04.016
|
| [31] |
Peichl, M., Leava, N. A., & Kiely, G. (2012). Above- and belowground ecosystem biomass, carbon and nitrogen allocation in recently afforested grassland and adjacent intensively managed grassland. Plant and Soil, 350(1–2), 281–296. https://doi.org/10.1007/s11104-011-0905-9
|
| [32] |
Poblete-Grant, P., Suazo- Hernández, J., Condron, L., Rumpel, C., Demanet, R., Malone, S. L., & Mora, M. L. L. (2020). Soil available P, soil organic carbon and aggregation as affected by long-term poultry manure application to Andisols under pastures in Southern Chile. Geoderma Regional, 21, e00271. https://doi.org/10.1016/j.geodrs.2020.e00271
|
| [33] |
Postma-Blaauw, M. B., de Goede, R. G. M., Bloem, J., Faber, J. H., & Brussaard, L. (2010). Soil biota community structure and abundance under agricultural intensification and extensification. Ecology, 91(2), 460–473. https://doi.org/10.1890/09-0666.1
|
| [34] |
Rasmussen, C., Heckman, K., Wieder, W. R., Keiluweit, M., Lawrence, C. R., Berhe, A. A., Blankinship, J. C., Crow, S. E., Druhan, J. L., Hicks Pries, C. E., Marin-Spiotta, E., Plante, A. F., Schädel, C., Schimel, J. P., Sierra, C. A., Thompson, A., & Wagai, R. (2018). Beyond clay: Towards an improved set of variables for predicting soil organic matter content. Biogeochemistry, 137(3), 297–306. https://doi.org/10.1007/s10533-018-0424-3
|
| [35] |
Ribeiro, D. O., Castoldi, G., Freiberger, M. B., Silva, M. A. S. D., & Rodrigues, C. R. (2022). Physical fractionation and carbon and nitrogen stocks in soil after poultry waste applications. Revista Caatinga, 35(3), 667–676. https://doi.org/10.1590/1983-21252022v35n318rc
|
| [36] |
Rumpel, C., Crème, A., Ngo, P. T., Velásquez, G., Mora, M. L., & Chabbi, A. (2015). The impact of grassland management on biogeochemical cycles involving carbon, nitrogen and phosphorus. Journal of Soil Science and Plant Nutrition, 15(2), 353–371. https://doi.org/10.4067/S0718-95162015005000034
|
| [37] |
Russell, E. W. (1977). The rôle of organic matter in soil fertility. Philosophical Transactions of the Royal Society B, 181, 209–219. https://doi.org/10.1098/rstb.1977.0134
|
| [38] |
Schmidt, M. W. I., Torn, M. S., Abiven, S., Dittmar, T., Guggenberger, G., Janssens, I. A., Kleber, M., Kögel-Knabner, I., Lehmann, J., Manning, D. A. C., Nannipieri, P., Rasse, D. P., Weiner, S., & Trumbore, S. E. (2011). Persistence of soil organic matter as an ecosystem property. Nature, 478(7367), 49–56. https://doi.org/10.1038/nature10386
|
| [39] |
Sizmur, T., Collins, C., Clark, J., & Antony, D. (2022). Data for: The chemical and physical stability of soil organic carbon in the top 1 m of the soil profile under different land uses in England (Version 1) [Data set]. Mendeley Data. https://doi.org/10.17632/v8sztp867f.1
|
| [40] |
Sollenberger, L. E., Kohmann, M. M., Dubeux, Jr., J. C. B., & Silveira, M. L. (2019). Grassland management affects delivery of regulating and supporting ecosystem services. Crop Science, 59(2), 441–459. https://doi.org/10.2135/cropsci2018.09.0594
|
| [41] |
USDA-NASS (National Agricultural Statistics Service). (2022). 2017 census of agriculture. United States Department of Agriculture. https://www.nass.usda.gov/AgCensus/
|
| [42] |
Wander, M. M., Bidart, M. G., & Aref, S. (1998). Tillage impacts on depth distribution of total and particulate organic matter in three Illinois soils. Soil Science Society of America Journal, 62(6), 1704–1711. https://doi.org/10.2136/sssaj1998.03615995006200060031x
|
| [43] |
Weaver, J. E., & Darland, R. W. (1949). Soil-root relationships of certain native grasses in various soil types. Ecological Monographs, 19, 303–338. https://doi.org/10.2307/1943273
|
| [44] |
Zhou, Y., Watts, S. E., Boutton, T. W., & Archer, S. R. (2019). Root density distribution and biomass allocation of co-occurring woody plants on contrasting soils in a subtropical savanna parkland. Plant and Soil, 438(1–2), 263–279. https://doi.org/10.1007/s11104-019-04018-9
|
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2025 Published 2025. This article is a U.S. Government work and is in the public domain in the USA. Grassland Research published by John Wiley & Sons Australia, Ltd on behalf of Chinese Grassland Society and Lanzhou University.
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