MiRNA let-7i promotes radiation-induced pulmonary epithelial-mesenchymal transition by targeting IL-10

Shenghui Zhou; Xin Liang; Zewen Sun; Xueping Li; Jiaojiao Zhu; Zhihua Yang; Xiujie Pan; Yilong Wang; Yongqing Gu; Maoxiang Zhu

doi:10.1007/s42764-022-00089-8

Genome Instability & Disease ›› 2022, Vol. 3 ›› Issue (6) : 271-284. DOI: 10.1007/s42764-022-00089-8

Original Research Paper

MiRNA let-7i promotes radiation-induced pulmonary epithelial-mesenchymal transition by targeting IL-10

Shenghui Zhou¹^,² ,
Xin Liang²^,³ ,
Zewen Sun² ,
Xueping Li²^,⁴ ,
Jiaojiao Zhu² ,
Zhihua Yang² ,
Xiujie Pan² ,
Yilong Wang² ,
Yongqing Gu¹^,²^,⁴^,^j ,
Maoxiang Zhu²^,^k

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Abstract

Radiation-induced pulmonary fibrosis (RIPF) is a common complication of thoracic tumors, lacking targeted therapeutic strategies, which seriously affects the quality of life. Numerous studies have confirmed that epithelial to mesenchymal transition (EMT) plays an important role in RIPF, but the specific mechanism remains unclear. We verified by mice lung tissue miRNA microarray screen combined with alveolar type II epithelial cells (AECII) and found that miRNA-let-7i was significantly highly expressed after ionizing radiation (IR). It was also confirmed that miRNA-let-7i inhibited its expression by targeted binding to the 3ʹUTR of IL-10, promoting the phosphorylation activation of AKT, and subsequently inducing the EMT of AECII. In conclusion, our study confirmed the role and specific mechanism of miRNA-let-7i promoting AECII cells EMT to participate in RIPF and provided a new target for the prevention and treatment of RIPF.

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Shenghui Zhou, Xin Liang, Zewen Sun, Xueping Li, Jiaojiao Zhu, Zhihua Yang, Xiujie Pan, Yilong Wang, Yongqing Gu, Maoxiang Zhu. MiRNA let-7i promotes radiation-induced pulmonary epithelial-mesenchymal transition by targeting IL-10. Genome Instability & Disease, 2022, 3(6): 271‒284 https://doi.org/10.1007/s42764-022-00089-8