Magnetic Solid Phase Extraction of Paeonol and Paeonoflorinfrom Moutan Bark with Magnetic Graphene Oxide

Abstract

Herbal medicine plays animportant role in modern medicine and separation of the active ingredients fromherbal medicine is vital for convenient and safe usage. Paeonol andpaeonoflorin are the active ingredients in the widely used herbal medicine ofmoutan bark. In this study, the composite of graphene oxide-Fe₃O₄ nanoparticles (GO-Fe₃O₄) was synthesized and used as a magneticabsorbent to extract paeonol and paeonoflorin from the herbal medicine ofmoutan bark. The adsorption of paeonol and paeoniflorin on GO-Fe₃O₄ rapidly reached equilibrium (within 10 min) due to the high absorptioncapability of GO. Thermodynamics and kinetics for the absorption process werestudied. The optimal condition for the elution of the target compound from GO-Fe₃O₄ was the use of 2 mL of a mixed solvent (methanol and dichloromethane, 1:1 byvolume) with 0.2% formic acid for 5 min. The GO-Fe₃O₄ adsorbent possesses the advantages of rapid adsorption and convenientseparation. GO-Fe₃O₄ can be used over 6 times withoutlosing absorbing capacity. This method is efficient, convenient and rapid, thuspossesses a high potential for the separation of active ingredients from herbalmedicine.

Keywords

Graphene oxide / Fe₃O₄ / Extraction / Paeonol / Paeoniflorin

Acknowledgments

Financial support from Hubei Three Gorges Laboratory through grant No. SK211007 is acknowledged.

Author Contributions

J.J.: Writing—Original Draft Preparation, Data Curation. M.L. Formal Analysis, X.H. Investigation, J.Y.: Methodology, R.C.: Investigation, Formal analysis. Q.Z.: Writing—Review & Editing, Funding Acquisition, Methodology.

Ethics Statement

Not applicable.

Informed Consent Statement

Not applicable.

Funding

This study was funded by the Hubei Three Gorges Laboratory through grant No.SK211007.

Declaration of competing Interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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