This study aimed to develop a clean-label 3D-printable food ink using a formulation based on Khanom Piak Poon, a traditional Thai dessert, enriched with carrot powder (CP) for enhanced nutritional value. Unlike conventional food printing inks that contain additive hydrocolloids, this ink relies on the natural reactions between starch, sugar, and dietary fiber. Response surface methodology (RSM), combined with a Box-Behnken design, was applied to optimize a formulation consisting of rice flour (RF), coconut sugar (CS), and CP. The results showed that interactions among these components significantly affected rheological properties and printing accuracy. The optimal formulation had an RF:CS:CP ratio of 9.8:5.4:6.3, achieving 93.6% printing accuracy, which was close to the predicted value (R2 = 0.99). Rheological analysis revealed that successful printing depends on a specific balance between shear-thinning behavior and viscoelasticity. The optimal formulation exhibited a viscosity of approximately 1000 Pa·s at a shear rate of 0.1 s−1 and a storage modulus (G’) of 5000 Pa at an angular frequency of 10 rad/s. This defined rheological range allows for optimal flow under pressure while ensuring rapid structural recovery after printing. Textural characterization indicates that the optimized printed gel had a soft texture suitable for easy consumption, comparable to the traditional dessert, but with improved nutritional value. This study provides a rheological framework for the 3D printing of clean-label, plant-based food, demonstrating that the matrix of the traditional Thai dessert can be successfully modernized without compromising its original composition.
| [1] |
Adamczyk, G., M. Krystyjan, and G. Jaworska. 2020. “The Effect of the Addition of Dietary Fibers From Apple and Oat on the Rheological and Textural Properties of Waxy Potato Starch.” Polymers 12, no. 2: 321. https://doi.org/10.3390/polym12020321.
|
| [2] |
Ahmad, M., T. A. Wani, S. M. Wani, F. A. Masoodi, and A. Gani. 2016. “Incorporation of Carrot Pomace Powder in Wheat Flour: Effect on Flour, Dough and Cookie Characteristics.” Journal of Food Science and Technology 53, no. 10: 3715-3724. https://doi.org/10.1007/s13197-016-2345-2.
|
| [3] |
Ahmadzadeh, S., T. Clary, A. Rosales, and A. Ubeyitogullari. 2024. “Upcycling Imperfect Broccoli and Carrots Into Healthy Snacks Using an Innovative 3D Food Printing Approach.” Food Science & Nutrition 12, no. 1: 84-93. https://doi.org/10.1002/fsn3.3820.
|
| [4] |
Al-Ansi, W., A. Ali Mahdi, Y. Li, H. Qian, and L. Wang. 2018. “Optimization and Acceptability Evaluation of Shapporah Biscuits Formulated by Different Ingredients: Using Response Surface Methodology (RSM).” Journal of Food and Nutrition Research 6, no. 3: 192-199. https://doi.org/10.12691/jfnr-6-3-9.
|
| [5] |
AOAC. 2000. Official Methods of Analysis of AOAC International (Method No. 925.45). 17th ed. Association of Official Analytical Chemists.
|
| [6] |
Bugday, Z. Y., A. Venkatachalam, P. D. Anderson, and R. G. M. van der Sman. 2024. “Rheology of Paste-Like Food Inks for 3D Printing: Effects of Nutrient and Water Content.” Current Research in Food Science 9: 100847. https://doi.org/10.1016/j.crfs.2024.100847.
|
| [7] |
Carvajal-Mena, N., G. Tabilo-Munizaga, M. Pérez-Won, C. Herrera-Lavados, R. Lemus-Mondaca, and L. Moreno-Osorio. 2023. “Evaluation of Physicochemical Properties of Starch-Protein Gels: Printability and Postprocessing.” LWT 182: 114797. https://doi.org/10.1016/j.lwt.2023.114797.
|
| [8] |
Chang, M.-Y., and H.-S. Chen. 2022. “Understanding Consumers’ Intentions to Purchase Clean Label Products: Evidence From Taiwan.” Nutrients 14, no. 18: 3684. https://doi.org/10.3390/nu14183684.
|
| [9] |
Chen, J., H. Sun, T. Mu, et al. 2022. “Effect of Temperature on Rheological, Structural, and Textural Properties of Soy Protein Isolate Pastes for 3D Food Printing.” Journal of Food Engineering 323: 110917. https://doi.org/10.1016/j.jfoodeng.2021.110917.
|
| [10] |
Coțovanu, I., and S. Mironeasa. 2021. “Impact of Different Amaranth Particle Sizes Addition Level on Wheat Flour Dough Rheology and Bread Features.” Foods 10, no. 7: 1539. https://doi.org/10.3390/foods10071539.
|
| [11] |
Derossi, A., R. Caporizzi, D. Azzollini, and C. Severini. 2018. “Application of 3D Printing for Customized Food. A Case on the Development of a Fruit-Based Snack for Children.” Journal of Food Engineering 220: 65-75. https://doi.org/10.1016/j.jfoodeng.2017.05.015.
|
| [12] |
Derossi, A., R. Caporizzi, M. Paolillo, and C. Severini. 2021. “Programmable Texture Properties of Cereal-Based Snack Mediated by 3D Printing Technology.” Journal of Food Engineering 289: 110160. https://doi.org/10.1016/j.jfoodeng.2020.110160.
|
| [13] |
Dick, A., X. Dong, B. Bhandari, and S. Prakash. 2021. “The Role of Hydrocolloids on the 3D Printability of Meat Products.” Food Hydrocolloids 119: 106879. https://doi.org/10.1016/j.foodhyd.2021.106879.
|
| [14] |
Fan, M., Y.-J. Choi, N. E. Wedamulla, et al. 2024. “Different Particle Sizes of Momordica charantia Leaf Powder Modify the Rheological and Textural Properties of Corn Starch-Based 3D Food Printing Ink.” Heliyon 10, no. 4: e24915. https://doi.org/10.1016/j.heliyon.2024.e24915.
|
| [15] |
García-Segovia, P., V. García-Alcaraz, S. Balasch-Parisi, and J. Martínez-Monzó. 2020. “3D Printing of Gels Based on Xanthan/Konjac Gums.” Innovative Food Science & Emerging Technologies 64: 102343. https://doi.org/10.1016/j.ifset.2020.102343.
|
| [16] |
Grace, N. C. F., and C. Jeyakumar Henry. 2020. “The Physicochemical Characterization of Unconventional Starches and Flours Used in Asia.” Foods 9, no. 2: 182. https://doi.org/10.3390/foods9020182.
|
| [17] |
He, A., J. Xu, Q. Hu, et al. 2023. “Effects of Gums on 3D Printing Performance of Pleurotus Eryngii Powder.” Journal of Food Engineering 351: 111514. https://doi.org/10.1016/j.jfoodeng.2023.111514.
|
| [18] |
In, J., H. Jeong, S. Song, and S. C. Min. 2021. “Determination of Material Requirements for 3D Gel Food Printing Using a Fused Deposition Modeling 3D Printer.” Foods 10, no. 10: 2272. https://doi.org/10.3390/foods10102272.
|
| [19] |
Jeon, E. Y., Y. G. Chun, and B.-K. Kim. 2024. “Investigation of Carrot/Squid Blends as Edible Inks for Extrusion 3D Printing: Effect of Hydrocolloids Incorporation.” Journal of Food Engineering 364: 111777. https://doi.org/10.1016/j.jfoodeng.2023.111777.
|
| [20] |
Kajzer, M., and A. Diowksz. 2021. “The Clean Label Concept: Novel Approaches in Gluten-Free Breadmaking.” Applied Sciences 11, no. 13: 6129. https://doi.org/10.3390/app11136129.
|
| [21] |
Kim, J., J. S. Kim, J.-H. Lim, and K.-D. Moon. 2024. “Effects of Isolated Pea Protein on Honeyed Red Ginseng Manufactured by 3D Printing for Patients With Dysphagia.” LWT 191: 115570. https://doi.org/10.1016/j.lwt.2023.115570.
|
| [22] |
Kim, J., J. S. Kim, and K.-D. Moon. 2025. “3D-Printed Rice Cake for Dysphagia Diet: Effect of Rice Flour/κ-Carrageenan/Curdlan Complex Gel on Structure, Swallowability, and Storage.” Future Foods 11: 100537. https://doi.org/10.1016/j.fufo.2024.100537.
|
| [23] |
Le-Bail, A., B. C. Maniglia, and P. Le-Bail. 2020. “Recent Advances and Future Perspective in Additive Manufacturing of Foods Based on 3D Printing.” Current Opinion in Food Science 35: 54-64. https://doi.org/10.1016/j.cofs.2020.01.009.
|
| [24] |
Lee, D., M. I. Saleh, and Y. Lee. 2025. “Effect of Sucrose on the Rheology and 3D Printability of Pregelatinized Rice Flour Paste.” Foods 14, no. 7: 1107. https://doi.org/10.3390/foods14071107.
|
| [25] |
Lian, W., Q. Hu, M. Qu, et al. 2023. “Impact of Insoluble Dietary Fiber and CaCl2 on Structural Properties of Soybean Protein Isolate-Wheat Gluten Composite Gel.” Foods 12, no. 9: 1890. https://doi.org/10.3390/foods12091890.
|
| [26] |
Liang, Y.-X., J.-B. Xu, L. Zhou, X. Li, L. Zhang, and F.-B. Meng. 2025. “Effects of Different Fruit Freeze-Dried Powders on the 3D Printing Properties of Peach Gum-Based Gummy Candy Gels.” Food Chemistry: X 27: 102464. https://doi.org/10.1016/j.fochx.2025.102464.
|
| [27] |
Lille, M., A. Nurmela, E. Nordlund, S. Metsä-Kortelainen, and N. Sozer. 2018. “Applicability of Protein and Fiber-Rich Food Materials in Extrusion-Based 3D Printing.” Journal of Food Engineering 220: 20-27. https://doi.org/10.1016/j.jfoodeng.2017.04.034.
|
| [28] |
Liu, H., Q. Hu, S. Yang, L. Liu, and X. Dong. 2025. “Temperature-Mediated Gel Texture Transformation in Starch Noodles: In Respect of Glass Transition Temperature Tg’.” Gels 11, no. 8: 639. https://doi.org/10.3390/gels11080639.
|
| [29] |
Liu, L., X. Yang, B. Bhandari, Y. Meng, and S. Prakash. 2020. “Optimization of the Formulation and Properties of 3D-Printed Complex Egg White Protein Objects.” Foods 9, no. 2: 164. https://doi.org/10.3390/foods9020164.
|
| [30] |
Liu, T., J. Zheng, J. Du, and G. He. 2024. “Food Processing and Nutrition Strategies for Improving the Health of Elderly People With Dysphagia: A Review of Recent Developments.” Foods 13, no. 2: 215. https://doi.org/10.3390/foods13020215.
|
| [31] |
Liu, Z., B. Bhandari, S. Prakash, S. Mantihal, and M. Zhang. 2019. “Linking Rheology and Printability of a Multicomponent Gel System of Carrageenan-Xanthan-Starch in Extrusion Based Additive Manufacturing.” Food Hydrocolloids 87: 413-424. https://doi.org/10.1016/j.foodhyd.2018.08.026.
|
| [32] |
Liu, Z., B. Bhandari, S. Prakash, and M. Zhang. 2018. “Creation of Internal Structure of Mashed Potato Construct by 3D Printing and Its Textural Properties.” Food Research International 111: 534-543. https://doi.org/10.1016/j.foodres.2018.05.075.
|
| [33] |
Ma, H., M. Liu, Y. Liang, et al. 2022. “Research Progress on Properties of Pre-Gelatinized Starch and Its Application in Wheat Flour Products.” Grain & Oil Science and Technology 5, no. 2: 87-97. https://doi.org/10.1016/j.gaost.2022.01.001.
|
| [34] |
Matas, A., M. D. Molina-Montero, M. Igual, P. García-Segovia, and J. Martínez-Monzó. 2022. “Printability Prediction of Three Gels for 3D Food Printing.” Biology and Life Sciences Forum 18, no. 1: 25. https://doi.org/10.3390/Foods2022-12986.
|
| [35] |
Mauro, R. R., A. J. Vela, and F. Ronda. 2023. “Impact of Starch Concentration on the Pasting and Rheological Properties of Gluten-Free Gels. Effects of Amylose Content and Thermal and Hydration Properties.” Foods 12, no. 12: 2281. https://doi.org/10.3390/foods12122281.
|
| [36] |
Mohamed, A. A., M. S. Alamri, H. Al-Quh, et al. 2024. “Effect of Shearing and Annealing on the Pasting Properties of Different Starches.” Gels 10, no. 6: 350. https://doi.org/10.3390/gels10060350.
|
| [37] |
Mohamed, A. A., S. Hussain, M. S. Alamri, A. A. Abdo Qasem, M. A. Ibraheem, and M. I. Alhazmi. 2019. “Dynamic Rheological Properties of Corn Starch-Date Syrup Gels.” Journal of Food Science and Technology 56, no. 2: 927-936. https://doi.org/10.1007/s13197-018-03558-9.
|
| [38] |
Outrequin, T. C. R., C. Gamonpilas, W. Siriwatwechakul, and P. Sreearunothai. 2023. “Extrusion-Based 3D Printing of Food Biopolymers: A Highlight on the Important Rheological Parameters to Reach Printability.” Journal of Food Engineering 342: 111371. https://doi.org/10.1016/j.jfoodeng.2022.111371.
|
| [39] |
Pan, J., X. Chen, Y. Zhu, et al. 2024. “Design and Development of Dual-Extruder Food 3D Printer Based on Selective Compliance Assembly Robot Arm and Printing of Various Inks.” Journal of Food Engineering 370: 111973. https://doi.org/10.1016/j.jfoodeng.2024.111973.
|
| [40] |
Pan, Y., Q. Sun, Y. Liu, et al. 2021. “The Relationship Between Rheological and Textural Properties of Shrimp Surimi Adding Starch and 3D Printability Based on Principal Component Analysis.” Food Science & Nutrition 9, no. 6: 2985-2999. https://doi.org/10.1002/fsn3.2257.
|
| [41] |
Pant, A., A. Y. Lee, R. Karyappa, et al. 2021. “3D Food Printing of Fresh Vegetables Using Food Hydrocolloids for Dysphagic Patients.” Food Hydrocolloids 114: 106546. https://doi.org/10.1016/j.foodhyd.2020.106546.
|
| [42] |
Pant, A., P. X. Ni Leam, C. K. Chua, and U.-X. Tan. 2023. “Valorisation of Vegetable Food Waste Utilising Three-Dimensional Food Printing.” Virtual and Physical Prototyping 18, no. 1: e2146593. https://doi.org/10.1080/17452759.2022.2146593.
|
| [43] |
Pereira, J., H. Hu, L. Xing, W. Zhang, and G. Zhou. 2019. “Influence of Rice Flour, Glutinous Rice Flour, and Tapioca Starch on the Functional Properties and Quality of an Emulsion-Type Cooked Sausage.” Foods 9, no. 1: 9. https://doi.org/10.3390/foods9010009.
|
| [44] |
Pérez-Troncoso, D., D. M. Epstein, and J. A. Castañeda-García. 2021. “Consumers’ Preferences and Willingness to Pay for Personalised Nutrition.” Applied Health Economics and Health Policy 19, no. 5: 757-767. https://doi.org/10.1007/s40258-021-00647-3.
|
| [45] |
Pulatsu, E., and M. Lin. 2021. “A Review on Customizing Edible Food Materials Into 3D Printable Inks: Approaches and Strategies.” Trends in Food Science & Technology 107: 68-77. https://doi.org/10.1016/j.tifs.2020.11.023.
|
| [46] |
Qin, Z., Y. Yang, Z. Zhang, et al. 2025. “A Critical Review: Gel-Based Edible Inks for 3D Food Printing: Materials, Rheology-Geometry Mapping, and Control.” Gels 11, no. 10: 780. https://doi.org/10.3390/gels11100780.
|
| [47] |
da Quinta, N., A. B. Baranda, Y. Ríos, R. Llorente, A. B. Naranjo, and I. Martinez de Marañón. 2024. “Children's Physiological and Behavioural Response During the Observation, Olfaction, Manipulation, and Consumption of Food Products With Varied Textures. Part 2: Solid Products.” Food Quality and Preference 115: 105120. https://doi.org/10.1016/j.foodqual.2024.105120.
|
| [48] |
Quispe Santivañez, G. W., H. J. Javier Ninahuaman, J. Paucarchuco Soto, M. T. Pedrosa Silva Clerici, and R. Salvador-Reyes. 2025. “Optimization of 3D Extrusion Printing Parameters for Raw and Extruded Dehulled Andean Fava Bean Flours Using Response Surface Methodology (RSM).” Foods 14, no. 5: 715. https://doi.org/10.3390/foods14050715.
|
| [49] |
Said, N. S., I. F. Olawuyi, and W. Y. Lee. 2023. “Pectin Hydrogels: Gel-Forming Behaviors, Mechanisms, and Food Applications.” Gels 9, no. 9: 732. https://doi.org/10.3390/gels9090732.
|
| [50] |
De Salvo, M. I., C. A. Palla, and I. M. Cotabarren. 2024. “Development of Beetroot Powder-Enriched Inks for 3D Food Printing Based on Hydrogel/Oleogel Bigels.” Food Bioscience 62: 105278. https://doi.org/10.1016/j.fbio.2024.105278.
|
| [51] |
Seetapan, N., N. Limparyoon, C. Gamonpilas, P. Methacanon, and A. Fuongfuchat. 2015. “Effect of Cryogenic Freezing on Textural Properties and Microstructure of Rice Flour/Tapioca Starch Blend Gel.” Journal of Food Engineering 151: 51-59. https://doi.org/10.1016/j.jfoodeng.2014.11.025.
|
| [52] |
Severini, C., A. Derossi, I. Ricci, R. Caporizzi, and A. Fiore. 2018. “Printing a Blend of Fruit and Vegetables. New Advances on Critical Variables and Shelf Life of 3D Edible Objects.” Journal of Food Engineering 220: 89-100. https://doi.org/10.1016/j.jfoodeng.2017.08.025.
|
| [53] |
Shahrubudin, N., T. C. Lee, and R. Ramlan. 2019. “An Overview on 3D Printing Technology: Technological, Materials, and Applications.” Procedia Manufacturing 35: 1286-1296. https://doi.org/10.1016/j.promfg.2019.06.089.
|
| [54] |
Sharifi, Z., A. Jebelli Javan, M. A. Hesarinejad, and M. Parsaeimehr. 2023. “Application of Carrot Waste Extract and Lactobacillus plantarum in Alyssum homalocarpum Seed Gum-Alginate Beads to Create a Functional Synbiotic Yogurt.” Chemical and Biological Technologies in Agriculture 10, no. 1: 3. https://doi.org/10.1186/s40538-022-00377-1.
|
| [55] |
Strother, H., R. Moss, and M. B. McSweeney. 2020. “Comparison of 3D Printed and Molded Carrots Produced With Gelatin, Guar Gum and Xanthan Gum.” Journal of Texture Studies 51, no. 6: 852-860. https://doi.org/10.1111/jtxs.12545.
|
| [56] |
Tejada-Ortigoza, V., and E. Cuan-Urquizo. 2022. “Towards the Development of 3D-Printed Food: A Rheological and Mechanical Approach.” Foods 11, no. 9: 1191. https://doi.org/10.3390/foods11091191.
|
| [57] |
Theagarajan, R., J. A. Moses, and C. Anandharamakrishnan. 2020. “3D Extrusion Printability of Rice Starch and Optimization of Process Variables.” Food and Bioprocess Technology 13, no. 6: 1048-1062. https://doi.org/10.1007/s11947-020-02453-6.
|
| [58] |
Vashisht, P., L. Singh, G. S. Saini, et al. 2025. “Review of Potential Clean Label Ingredients in Yogurt, Cheese and Ice Cream Sector.” Food and Humanity 4: 100474. https://doi.org/10.1016/j.foohum.2024.100474.
|
| [59] |
Watcharakitti, J., E. E. Win, J. Nimnuan, and S. M. Smith. 2022. “Modified Starch-Based Adhesives: A Review.” Polymers 14, no. 10: 2023. https://doi.org/10.3390/polym14102023.
|
| [60] |
Wongkhueng, K., B. Thumthanaruk, Y. Wandee, B. Lamsal, and V. Rungsardthong. 2025. “Pasting Profiles and Gel Properties of Rice Flour Blended With Native, Cross-Linked, and Acetylated Tapioca Starch.” E3S Web of Conferences 610: 02002. https://doi.org/10.1051/e3sconf/202561002002.
|
| [61] |
Woodbury, T. J., S. L. Pitts, A. M. Pilch, P. Smith, and L. J. Mauer. 2023. “Mechanisms of the Different Effects of Sucrose, Glucose, Fructose, and a Glucose-Fructose Mixture on Wheat Starch Gelatinization, Pasting, and Retrogradation.” Journal of Food Science 88, no. 1: 293-314. https://doi.org/10.1111/1750-3841.16414.
|
| [62] |
Yang, G., Y. Tao, P. Wang, X. Xu, and X. Zhu. 2022. “Optimizing 3D Printing of Chicken Meat by Response Surface Methodology and Genetic Algorithm: Feasibility Study of 3D Printed Chicken Product.” LWT 154: 112693. https://doi.org/10.1016/j.lwt.2021.112693.
|
| [63] |
Zhang, Y., A. Y. Lee, K. Pojchanun, et al. 2022. “Systematic Engineering Approach for Optimization of Multi-Component Alternative Protein-Fortified 3D Printing Food Ink.” Food Hydrocolloids 131: 107803. https://doi.org/10.1016/j.foodhyd.2022.107803.
|
| [64] |
Zhao, J., W. Xie, Z. Chen, Y. Zheng, and S. Li. 2025. “Enhancement of Dough Processing and Steamed Bread Quality With Modified Soybean Residue Dietary Fiber.” Foods 14, no. 3: 346. https://doi.org/10.3390/foods14030346.
|
| [65] |
Zhao, P., D. Kou, R. Qiu, et al. 2025. “Development of Soy Protein Emulsion Gels-Based 3D Printed Dysphagia Foods: Effects of the Egg White Protein Supplementation.” Food Hydrocolloids 160: 110737. https://doi.org/10.1016/j.foodhyd.2024.110737.
|
| [66] |
Zhou, D.-N., B. Zhang, B. Chen, and H.-Q. Chen. 2017. “Effects of Oligosaccharides on Pasting, Thermal and Rheological Properties of Sweet Potato Starch.” Food Chemistry 230: 516-523. https://doi.org/10.1016/j.foodchem.2017.03.088.
|
| [67] |
Zhu, S., M. A. Stieger, A. J. van der Goot, and M. A. Schutyser. 2019. “Extrusion-Based 3D Printing of Food Pastes: Correlating Rheological Properties With Printing Behaviour.” Innovative Food Science & Emerging Technologies 58: 102214. https://doi.org/10.1016/j.ifset.2019.102214.
|
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2026 The Author(s). Food Bioengineering published by John Wiley & Sons Australia, Ltd on behalf of State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology.