Relationship Between Gluten Structural Properties and Noodle Texture: Insights From Seven Wheat Varieties

Abstract

A comprehensive understanding of how gluten properties affect noodle texture remains limited. This study examined the impact of gluten physicochemical and structural properties on noodle texture. Seven wheat varieties from China, France, Canada, and Australia were utilized. Results indicated that increased surface hydrophobicity and higher β-sheet content determined based on the relative levels of each property among the tested varieties reduced gluten water retention, lowering noodle adhesiveness. Greater surface hydrophobicity also enhanced gluten thermal stability, improving noodle chewiness, hardness, and tensile properties. In contrast, higher α-helix content increased solubility, while a greater proportion of high molecular weight gluten subunits (HMW-GS) strengthened the gluten network, enhancing hardness and elasticity. Among the tested varieties, Australian durum wheat (AD) exhibited superior elasticity and balanced texture, with hardness (376.43 g), chewiness (267.13 g), adhesiveness (27.49), and resilience (0.815). These properties were linked to its high water-holding capacity (3.03 g/g), solubility (0.3 mg/mL), and thermal stability (T_d = 58.18°C). These findings clarify the role of gluten in noodle texture and establish protein-based criteria for wheat selection in processing.

Keywords

correlation analysis / gluten / noodles / textural properties

References

[1] AACC. 2000. Approved Methods of the American Association of Cereal Chemists (10th). Author.

[2] Akharume, F., D. Santra, and A. Adedeji. 2020. “Physicochemical and Functional Properties of Proso Millet Storage Protein Fractions.” Food Hydrocolloids 108: 105497. https://doi.org/10.1016/j.foodhyd.2019.105497.

[3] An, D., H. Li, D. Li, et al. 2022. “The Relation Between Wheat Starch Properties and Noodle Springiness: From the View of Microstructure Quantitative Analysis of Gluten-Based Network.” Food Chemistry 393: 133396. https://doi.org/10.1016/j.foodchem.2022.133396.

[4] Barak, S., D. Mudgil, and B. S. Khatkar. 2014. “Effect of Compositional Variation of Gluten Proteins and Rheological Characteristics of Wheat Flour on the Textural Quality of White Salted Noodles.” International Journal of Food Properties 17, no. 4: 731–740. https://doi.org/10.1080/10942912.2012.675611.

[5] Cai, M., C. Shen, Y. Li, S. Xiong, and F. Li. 2023. “The Quality Characteristics Comparison of Stone-Milled Dried Whole Wheat Noodles, Dried Wheat Noodles, and Commercially Dried Whole Wheat Noodles.” Foods 12, no. 1: 55. https://doi.org/10.3390/foods12010055.

[6] Chian, F. M., L. Kaur, T. Astruc, et al. 2021. “Shockwave Processing of Beef Brisket in Conjunction With Sous Vide Cooking: Effects on Protein Structural Characteristics and Muscle Microstructure.” Food Chemistry 343: 128500. https://doi.org/10.1016/j.foodchem.2020.128500.

[7] Cho, S.-W., C.-S. Kang, H. S. Ko, B.-K. Baik, K.-M. Cho, and C. S. Park. 2018. “Influence of Protein Characteristics and the Proportion of Gluten on End-Use Quality in Korean Wheat Cultivars.” Journal of Integrative Agriculture 17, no. 8: 1706–1719. https://doi.org/10.1016/S2095-3119(17)61822-7.

[8] Dewan, A., N. Chaudhary, and B. S. Khatkar. 2022. “Effects of Wheat Gliadin and Glutenin Fractions on Dough Properties, Oil Uptake, and Microstructure of Instant Noodles.” Journal of Food Processing and Preservation 46, no. 12: e17100. https://doi.org/10.1111/jfpp.17100.

[9] Ding, X., Z.-Y. Quan, W.-P. Chang, L. Li, and J.-Y. Qian. 2024. “Effect of Egg White Protein on the Protein Structure of Highland Barley Noodles During Processing.” Food Chemistry 433: 137320. https://doi.org/10.1016/j.foodchem.2023.137320.

[10] Dizlek, H., and J. M. Awika. 2023. “Determination of Basic Criteria That Influence the Functionality of Gluten Protein Fractions and Gluten Complex on Roll Bread Characteristics.” Food Chemistry 404: 134648. https://doi.org/10.1016/j.foodchem.2022.134648.

[11] Gao, X., J. Tong, L. Guo, et al. 2020. “Influence of Gluten and Starch Granules Interactions on Dough Mixing Properties in Wheat (Triticum aestivum L.).” Food Hydrocolloids 106: 105885. https://doi.org/10.1016/j.foodhyd.2020.105885.

[12] Grossmann, L., and D. J. McClements. 2023. “Current Insights Into Protein Solubility: A Review of Its Importance for Alternative Proteins.” Food Hydrocolloids 137: 108416. https://doi.org/10.1016/j.foodhyd.2022.108416.

[13] Hossain Brishti, F., S. Y. Chay, K. Muhammad, et al. 2021. “Structural and Rheological Changes of Texturized Mung Bean Protein Induced by Feed Moisture During Extrusion.” Food Chemistry 344: 128643. https://doi.org/10.1016/j.foodchem.2020.128643.

[14] Iwaki, S., K. Hayakawa, B.-X. Fu, and C. Otobe. 2021. “Changes in Hydrophobic Interactions Among Gluten Proteins During Dough Formation.” Processes 9, no. 7: 1244. https://doi.org/10.3390/pr9071244.

[15] Jeon, S., B.-K. Baik, and M. Kweon. 2019. “Solvent Retention Capacity Application to Assess Soft Wheat Flour Quality for Making White-Salted Noodles.” Cereal Chemistry 96, no. 3: 497–507. https://doi.org/10.1002/cche.10150.

[16] Jiang, Y., X. Zhou, Y. Zheng, D. Wang, Y. Deng, and Y. Zhao. 2021. “Impact of Ultrasonication/Shear Emulsifying/Microwave-Assisted Enzymatic Extraction on Rheological, Structural, and Functional Properties of Akebia trifoliata (Thunb.) Koidz. Seed Protein Isolates.” Food Hydrocolloids 112: 106355. https://doi.org/10.1016/j.foodhyd.2020.106355.

[17] Kaur, A., K. Shevkani, M. Katyal, N. Singh, A. K. Ahlawat, and A. M. Singh. 2016. “Physicochemical and Rheological Properties of Starch and Flour From Different Durum Wheat Varieties and Their Relationships With Noodle Quality.” Journal of Food Science and Technology 53, no. 4: 2127–2138. https://doi.org/10.1007/s13197-016-2202-3.

[18] Kovacs, M. I. P., B. X. Fu, S. M. Woods, and K. Khan. 2004. “Thermal Stability of Wheat Gluten Protein: Its Effect on Dough Properties and Noodle Texture.” Journal of Cereal Science 39, no. 1: 9–19. https://doi.org/10.1016/S0733-5210(03)00058-4.

[19] Kurotobi, T., T. Hoshino, Y. Kazami, F. Hayakawa, and Y. Hagura. 2018. “Relationship Between Sensory Analysis for Texture and Instrument Measurements in Model Strawberry Jam.” Journal of Texture Studies 49, no. 4: 359–369. https://doi.org/10.1111/jtxs.12348.

[20] Lagrain, B., B. G. Thewissen, K. Brijs, and J. A. Delcour. 2008. “Mechanism of Gliadin–Glutenin Cross-Linking During Hydrothermal Treatment.” Food Chemistry 107, no. 2: 753–760. https://doi.org/10.1016/j.foodchem.2007.08.082.

[21] Lambrecht, M. A., L. J. Deleu, I. Rombouts, and J. A. Delcour. 2018. “Heat-Induced Network Formation Between Proteins of Different Sources in Model Systems, Wheat-Based Noodles and Pound Cakes.” Food Hydrocolloids 79: 352–370. https://doi.org/10.1016/j.foodhyd.2017.12.032.

[22] Lambrecht, M. A., I. Rombouts, M. A. Nivelle, and J. A. Delcour. 2017. “The Impact of Protein Characteristics on the Protein Network in and Properties of Fresh and Cooked Wheat-Based Noodles.” Journal of Cereal Science 75: 234–242. https://doi.org/10.1016/j.jcs.2017.04.014.

[23] Li, H., J. Wang, L. Pan, and Q. Lu. 2019. “Effect of Amino and Thiol Groups of Wheat Gluten on the Quality Characteristics of Chinese Noodles.” Journal of Food Science and Technology 56, no. 6: 2825–2835. https://doi.org/10.1007/s13197-019-03688-8.

[24] Li, M., S. Dhital, and Y. Wei. 2017. “Multilevel Structure of Wheat Starch and Its Relationship to Noodle Eating Qualities.” Comprehensive Reviews in Food Science and Food Safety 16, no. 5: 1042–1055. https://doi.org/10.1111/1541-4337.12272.

[25] Li, M., Q. Yue, C. Liu, et al. 2021. “Interaction Between Gliadin/Glutenin and Starch Granules in Dough During Mixing.” LWT 148: 111624. https://doi.org/10.1016/j.lwt.2021.111624.

[26] Li, R., L. Wang, D. Hou, and S. Zhou. 2024. “Egg White Improved the Quality of Noodles With High Mung Bean Content by Protein Aggregation Behavior.” Cereal Chemistry 101, no. 1: 120–130. https://doi.org/10.1002/cche.10728.

[27] Li, S., W. He, D. Tang, G. Chen, and Y. Chen. 2024. “Effect of Ultrasonic Power Density on the Quality of Fresh Wet Noodles.” Journal of Cereal Science 116: 103863. https://doi.org/10.1016/j.jcs.2024.103863.

[28] Li, X., P. Liu, Z. Bai, et al. 2024. “Ultrasound-Assisted Process of Dough for Noodle Production: Textural Properties, Moisture Distribution, Microstructure and Cooking Quality.” Journal of Cereal Science 119: 104005. https://doi.org/10.1016/j.jcs.2024.104005.

[29] Li, Y., J. Fu, Q. Shen, and D. Yang. 2021. “High-Molecular-Weight Glutenin Subunits: Genetics, Structures, and Relation to End Use Qualities.” International Journal of Molecular Sciences 22, no. 1: 184. https://doi.org/10.3390/ijms22010184.

[30] Liu, M., M. Fan, H. Qian, Y. Li, and L. Wang. 2023. “Effect of Different Enzymes on Thermal and Structural Properties of Gluten, Gliadin, and Glutenin in Triticale Whole-Wheat Dough.” International Journal of Biological Macromolecules 253: 127384. https://doi.org/10.1016/j.ijbiomac.2023.127384.

[31] Ma, S., W. Han, L. Li, X. Zheng, and X. Wang. 2019. “The Thermal Stability, Structural Changeability, and Aggregability of Glutenin and Gliadin Proteins Induced by Wheat Bran Dietary Fiber.” Food & Function 10, no. 1: 172–179. https://doi.org/10.1039/C8FO01810C.

[32] Munch, M., L. Rezette, P. Buche, et al. 2025. “Dataset for Common Wheat (Triticum aestivum L.) Grain and Flour Characterization Using Classical and Advanced Analyses.” Data in Brief 59: 111375. https://doi.org/10.1016/j.dib.2025.111375.

[33] Mune Mune, M. A., D. S. Sogi, and S. R. Minka. 2018. “Response Surface Methodology for Investigating Structure–Function Relationship of Grain Legume Proteins.” Journal of Food Processing and Preservation 42, no. 2: e13524. https://doi.org/10.1111/jfpp.13524.

[34] Nadeem, M., F. M. Anjum, M. R. Khan, M. Sajjad, S. Hussain, and M. S. Arshad. 2016. “Electrophoretic Characteristics of Gluten Proteins as Influenced by Crop Year and Variety.” International Journal of Food Properties 19, no. 4: 897–910. https://doi.org/10.1080/10942912.2015.1045518.

[35] Paula, A. M., and A. C. Conti-Silva. 2014. “Texture Profile and Correlation Between Sensory and Instrumental Analyses on Extruded Snacks.” Journal of Food Engineering 121: 9–14. https://doi.org/10.1016/j.jfoodeng.2013.08.007.

[36] Pestorić, M., J. Mastilović, L. Pezo, et al. 2019. “Prediction of Commercial Spaghetti Quality Based on Sensory and Physicochemical Data.” Journal of Food Processing and Preservation 43, no. 11: e14172. https://doi.org/10.1111/jfpp.14172.

[37] Sun, J., M. Chen, X. Hou, et al. 2021. “Effect of Phosphate Salts on the Gluten Network Structure and Quality of Wheat Noodles.” Food Chemistry 358: 129895. https://doi.org/10.1016/j.foodchem.2021.129895.

[38] Wang, P., X. Zhao, R. Yang, et al. 2020. “Water-Extractable Arabinoxylan-Induced Changes in the Conformation and Polymerization Behavior of Gluten Upon Thermal Treatment.” Journal of Agricultural and Food Chemistry 68, no. 13: 4005–4016. https://doi.org/10.1021/acs.jafc.9b08122.

[39] Wang, Y.-H., Q.-Q. Zhang, Y.-Y. Guo, and F. Xu. 2021. “Effect of Flour Particle Size on the Qualities of Semi-Dried Noodles and Fine Dried Noodles.” Journal of Food Processing and Preservation 45, no. 7: e15632. https://doi.org/10.1111/jfpp.15632.

[40] Wang, Y. H., Y. R. Zhang, L. Qiao, W. M. Guo, Y. Y. Yang, and F. Xu. 2023. “Effects of Glutenin and Gliadin on the Surface Tackiness of Frozen Cooked Noodles.” Journal of Texture Studies 54: jtxs.12755. https://doi.org/10.1111/jtxs.12755.

[41] Wang, Y.-H., Y.-R. Zhang, X. Wang, et al. 2023. “Improving the Surface Tackiness of Frozen Cooked Noodles by the Addition of Glutenin, Gliadin, and Gluten.” LWT 179: 114637. https://doi.org/10.1016/j.lwt.2023.114637.

[42] Xiao, F., X. Zhang, M. Niu, et al. 2021. “Gluten Development and Water Distribution in Bread Dough Influenced by Bran Components and Glucose Oxidase.” LWT 137: 110427. https://doi.org/10.1016/j.lwt.2020.110427.

[43] Xu, W., D. J. McClements, Z. Xu, et al. 2024. “Optimization of Emulsion Properties of Chickpea Protein and Its Application in Food.” Journal of the American Oil Chemists' Society 101, no. 10: 971–980. https://doi.org/10.1002/aocs.12816.

[44] Yang, T., B. Wang, T. Lv, et al. 2025. “Investigating the Molecular Mechanism of High-Molecular-Weight Glutenin Subunit Affects Gluten Aggregation During Dough Mixing: Experimental Characterizations and Computational Simulations.” Food Chemistry 466: 142205. https://doi.org/10.1016/j.foodchem.2024.142205.

[45] Yao, M., M. Li, S. Dhital, Y. Tian, and B. Guo. 2020. “Texture and Digestion of Noodles With Varied Gluten Contents and Cooking Time: The View From Protein Matrix and Inner Structure.” Food Chemistry 315: 126230. https://doi.org/10.1016/j.foodchem.2020.126230.

[46] Zang, P., Y. Gao, P. Chen, C. Lv, and G. Zhao. 2022. “Recent Advances in the Study of Wheat Protein and Other Food Components Affecting the Gluten Network and the Properties of Noodles.” Foods 11, no. 23: 3824. https://doi.org/10.3390/foods11233824.

[47] Zhang, F., Y. Fu, Z. Liu, and Q. Shen. 2021. “Comparison of the Characteristics of Prolamins Among Foxtail Millet Varieties With Different Palatability: Structural, Morphological, and Physicochemical Properties.” International Journal of Biological Macromolecules 186: 194–205. https://doi.org/10.1016/j.ijbiomac.2021.07.051.

[48] Zhang, H., M. Fan, Y. Li, L. Wang, and H. Qian. 2023. “Study on the Prediction Model of Basic Components on the Quality of Buckwheat Noodles.” Journal of Texture Studies 54, no. 2: 245–257. https://doi.org/10.1111/jtxs.12733.

[49] Zhang, H., S. Liu, X. Feng, F. Ren, and J. Wang. 2023. “Effect of Hydrocolloids on Gluten Proteins, Dough, and Flour Products: A Review.” Food Research International 164: 112292. https://doi.org/10.1016/j.foodres.2022.112292.

[50] Zhang, H.-H., Q. Li, I. P. Claver, K.-X. Zhu, W. Peng, and H.-M. Zhou. 2010. “Effect of Cysteine on Structural, Rheological Properties and Solubility of Wheat Gluten by Enzymatic Hydrolysis.” International Journal of Food Science & Technology 45, no. 10: 2155–2161. https://doi.org/10.1111/j.1365-2621.2010.02384.x.

[51] Zhang, L., L.-J. Wang, W. Jiang, and J.-Y. Qian. 2017. “Effect of Pulsed Electric Field on Functional and Structural Properties of Canola Protein by Pretreating Seeds to Elevate Oil Yield.” LWT 84: 73–81. https://doi.org/10.1016/j.lwt.2017.05.048.

[52] Zhang, M., R. Jia, M. Ma, Q. Sun, M. Li, and F. Xie. 2024. “Effect of Sheeting Stress and Heating on the Molecular Chain Structure, Size, and Conformation of Gluten Proteins During Noodle Processing.” Food Hydrocolloids 146: 109266. https://doi.org/10.1016/j.foodhyd.2023.109266.

[53] Zhang, M., M. Ma, T. Yang, M. Li, and Q. Sun. 2022. “Dynamic Distribution and Transition of Gluten Proteins During Noodle Processing.” Food Hydrocolloids 123: 107114. https://doi.org/10.1016/j.foodhyd.2021.107114.

[54] Zhang, S., Y. Nie, H. Li, D. Zhu, H. Liu, and L. Yang. 2025. “The Gluten Aggregation Behavior and Quality of Whole Wheat Steamed Buns During Proofing.” Journal of Cereal Science 121: 104081. https://doi.org/10.1016/j.jcs.2024.104081.

[55] Zhang, X., Q. Guo, and W. Shi. 2023. “Ultrasound-Assisted Processing: Changes in Gel Properties, Water-Holding Capacity, and Protein Aggregation of Low-Salt Hypophthalmichthys molitrix Surimi by Soy Protein Isolate.” Ultrasonics Sonochemistry 92: 106258. https://doi.org/10.1016/j.ultsonch.2022.106258.

[56] Zhang, Y., C. Liu, J. Hong, et al. 2020. “Effect of Heat Treatment and Salt Addition on the Physicochemical Properties and Quality of Fresh Noodles.” International Journal of Food Science & Technology 55, no. 7: 2783–2793. https://doi.org/10.1111/ijfs.14531.

[57] Zhao, X., C. Hou, M. Tian, et al. 2020. “Effect of Water-Extractable Arabinoxylan With Different Molecular Weight on the Heat-Induced Aggregation Behavior of Gluten.” Food Hydrocolloids 99: 105318. https://doi.org/10.1016/j.foodhyd.2019.105318.

[58] Zhou, C., Y. Sun, Y. Yao, H. Li, and J. He. 2022. “Study of Noodle Quality Based on Protein Properties of Three Wheat Varieties.” Journal of Food Quality 2022: 1–13. https://doi.org/10.1155/2022/6383080.

[59] Zhou, H., Y. Zhang, Y. Yang, et al. 2023. “Effects of Low-Molecular-Weight Glutenin Subunit Encoded by Glu-A3 on Gluten and Chinese Fresh Noodle Quality.” Foods 12, no. 16: 3124. https://doi.org/10.3390/foods12163124.

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2025 The Author(s). Food Bioengineering published by John Wiley & Sons Australia, Ltd on behalf of State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology.