Eurotium cristatum (E. cristatum), commonly known as “golden flower”, is the dominant strain in the microbial fermentation process of Fu brick tea. E. cristatum has favorable biological characteristics, including enzyme production, antimicrobial properties, immune regulation, antitumor properties, fat reduction capabilities, and weight loss benefits. With its probiotic characteristics, E. cristatum can be combined with different varieties of tea substrates to make a variety of fermented teas. More importantly, in the process of tea fermentation, E. cristatum can secrete a variety of extracellular enzymes, including some hydrolytic enzymes and oxidoreductases. They metabolize and transform various chemical components in tea through a series of reactions such as oxidation, degradation, and condensation, which significantly affect the quality of tea. In this review, by summarizing its basic functional characteristics as well as its application in fermented tea, an in-depth analysis of the key problems existing in the fermentation application of E. cristatum is described and some beneficial suggestions are presented in order to provide a rich theoretical basis for the development and utilization of E. cristatum to a greater extent.
Acknowledgments
None.
Funding
This research was funded by the Central Government Guides Local Science and Technology Development Fund Project of Hebei Province (No. 226Z2801G), a Postdoctoral research project of Hebei Province (No. B2022005014), Science and Technology Innovation Projects of the Hebei Academy of Agriculture and Forestry Sciences (No. C21R0803, C22R0802), and Research Fund of Hebei University of Economics and Business (No. 2021SGYB04).
Conflict of interest
The authors declare no conflict of interests relevant to this study.
Author contributions
QZ summarized and organized this paper by searching and reading a large number of papers. JZ summarized the “Conclusions and perspectives” and mainly revised and edited the paper.
| [1] |
Qin JQ, Luo ML, Zhang X, Ling FQ. Research process on medi-cal value of Eurotium cristatum Sci Tech Food Ind 2018; 39(24):336-339. doi:10.13386/j.issn1002-0306.2018.24.057. (in Chinese).
|
| [2] |
Chen RY, Fan ZY, Pan YL, Gao Y, He C, Huang YX, et al. Eurotium cristatum biotransformation plant phenolic compounds and af-fects their biological activities: a review Food R&D 2022; 43(8):193-201. doi:10.12161/j.issn.1005-6521.2022.08.026. (in Chinese).
|
| [3] |
Cai ZA, Liu SC, Liu ZH, Hu ZR, Jia HX, Hu BH, et al. Cellulase enzymatic property of Eurotium cristatum from brick tea Tea Sci 2010; 30(1):57-62. doi:10.13305/j.cnki.jts.2010.01.003. (in Chinese).
|
| [4] |
Zheng WJ, Wan XC, Bao GH. Brick dark tea: a review of the manufac-ture, chemical constituents and bioconversion of the major chemical components during fermentation. Phytochem Rev 2015; 14:499-523. doi:10.1007/s11101-015-9402-8.
|
| [5] |
Jiang LZ, Wang L, Yang T, Zheng HN, Zhao JL, Xiang DB, et al. Research progress on Eurotium cristatum and its fermentation application Sci Tech Food Ind 2022; 43(4):454-462. doi:10.13386/j.issn1002-0306.2021030124. (in Chinese).
|
| [6] |
Huang Y, Shi R, Su EZ. Progress in the research and application of the Eurotium cristatum Bioprocess Eng 2017; 15(1):49-56. doi:10.3969/j.issn.1672-3678.2017.01.008. (in Chinese).
|
| [7] |
Wu F, Deng YZ, Qin YY, Jiang YH, Zhang LJ, Luo YC, et al. Isolation and identification of golden flower fungus in white tea Agric Res and Appl 2021; 34(6):1-6. (in Chinese).
|
| [8] |
Yang RJ, Wang QM, Peng WS, Ji AB, Zhang WJ, Yan L. Isolation and identification of microorganisms from 8 commercially avail-able “Jinhua” tea Tropical Agric 2019; 39(10):81-88. doi:10.12008/j.issn.1009-2196.2019.10.014. (in Chinese).
|
| [9] |
Guan PP, Munkhbayar E, Huang ZB, Li SG, Ji JH, Chen SH, et al. Isola-tion and identification of 23 fungal strains in tea leaves Jiangsu Agric Sci 2020; 48(16):285-286+288-290. doi:10.15889/j.issn.1002-1302.2020.16.053. (in Chinese).
|
| [10] |
Wang L, Tan GH, Pan QL, Peng XY, Zhang WM, Pang M, et al. Two spe-cies of aspergillus forming yellow cleistothecia popularly known as “golden flower” in dark brick tea of China Mycosystema 2015; 34(2):186-195. doi:10.13346/j.mycosystema.130275. (in Chinese).
|
| [11] |
Zhao RL, Xu W, Wu D, Jiang YH, Zhu Q. Microbial community di-versity of Fu brick tea produced in different regions by Illumina MiSeq technology. J Ecology 2017; 36(7):1865-1876. doi:10.13292/j.1000-4890.201707.012.
|
| [12] |
Liang XL. Isolation and identification of main molds and screening of dominant bacteria in Sichuan Fuzhuan brick tea Guang-dong Tea Ind 1996;(4):13-15. (in Chinese).
|
| [13] |
Tan YM, Wang YP, Ge YY, Ren XX, Wang YC, Liu ZY. Isolation and molecular identification of Aspergillus cristatus in fermented “fu-zhuan” brick tea from Guizhou Province Mycosystema 2017; 36(2):154-163. doi:10.13346/j.mycosystema.160125. (in Chinese).
|
| [14] |
Wu KW, Yang LN, Zhu LJ, Wang B, Liu H, He YT, et al. Advances on the application of Eurotium cristatum in Fermented Tea (in Chi-nese). Food R&D 2018; 39(1):189-193. doi:10.3969/j.issn.1005-6521.2018.01.037.
|
| [15] |
Li RX, Fang HF, Wang GH, Yang MH. Comparative analysis of the abil-ity of Aspergillus and Europium in dark tea to produce extracellular hydrolases Guangdong Agric Sci 2019; 46(1):108-114. doi:10.16768/j.issn.1004-874x.2019.01.016. (in Chinese).
|
| [16] |
Li JL, Hu BH, Zhao YB, Liu SC, Jiang YJ, Liu ZH. Anti-microbial Activity of Fermentation Supernatants of Eurotium cristatum Food Sci 2011; 32(11):157-160. (in Chinese).
|
| [17] |
Zhang FF, Liu F, Yuan C, Gao L, Wang Y, Ling PX, et al. Research on antimicrobial effects and characteristics of fermentation products of Eurotium cristatum F&D Products 2019; 21(1):62-65. doi:10.3969/j.issn.1672-979X.2019.01.015. (in Chinese).
|
| [18] |
Du FY, Li XM, Song JY, Li CS, Wang BG.Anthraquinone derivatives and an orsellinic acid ester from the marine alga-derived endophytic fun-gus Eurotium cristatum EN-22. Helv Chim Acta 2014; 97(7):973-978. doi:10.1002/hlca.201300358.
|
| [19] |
Xiao HS, Li TT, Tang H, Chen Y, Wang WJ, Liu C, et al. Antiiostatic activ-ity of propagule extracts of Eurotium cristatum Food and Fermn Ind 2020; 46(14):65-69. doi:10.13995/j.cnki.11-1802/ts.023391. (in Chinese).
|
| [20] |
Xie Z, Bai Y, Chen G, Rui Y, Chen D, Sun Y, et al. Modulation of gut homeostasis by exopolysaccharides from Aspergillus cristatus (MK346334), a strain of fungus isolated from Fuzhuan brick tea, contributes to immunomodulatory activity in cyclophosphamide-treated mice. Food Funct 2020; 11(12):10397-10412. doi:10.1039/d0fo02272a,PMID:33237077.
|
| [21] |
Almeida AP, Dethoup T, Singburaudom N, Lima R, Vasconcelos MH, Pinto M, et al. The in vitro anticancer activity of the crude extract of the sponge-associated fungus Eurotium cristatum and its second-ary metabolites. J Nat Pharm 2010; 1(1):25-29. doi:10.4103/2229-5119.73583.
|
| [22] |
Xu YM, Huang YM, Shi J, Zhou JL, Zhou HY, Pu H, et al. Study on anti-in-flammatory effect and mechanism of 2-6-H-5-B compound from Eu-rotium cristatum Mod Appl Sci in China 2022; 39(2):161-167. doi:10.13748/j.cnki.issn1007-7693.2022.02.004. (in Chinese).
|
| [23] |
Kou J, Shi LN, Ma TY. Effect of sweet potato tea fermented by Euro-tium cristatum on lipid metabolism and gut microbiota in hyperlipi-demic rats Food and Fermn Ind 2023; 49(16):231-239. doi:10.13995/j.cnki.11-1802/ts.034117. (in Chinese).
|
| [24] |
Liu W, Zhang GC, Li Q, Tian Y, Meng J, Zhong YT, et al. Study on identification and lipid-lowering effect of Eurotium cristatum in Fu tea from Jingyang county J Food Safety and Quality Testing 2019; 10(23):7907-7914. doi:10.19812/j.cnki.jfsq11-5956/ts.2019.23.018. (in Chinese).
|
| [25] |
Xiao J, Hou C, Chen X, Ying J, Zhu X, Wang LM, et al. Dark tea with pericarpium citri reticulatae improves glucose metabolism and li-pid metabolism disorders induced by high-fat diet in mice. Food Sci 2022; 43(5):133-142. doi:10.7506/spkx1002-6630-20210316-218.
|
| [26] |
Zhang HY. Research progress on application of bursa of coronoid in fermented tea Heilongjiang Sci 2018; 9(22):30-31. (in Chinese).
|
| [27] |
Zhang W, Wang MY, You JG, Ni L. Fermentation optimization of cul-ture medium and isolation and identification of antimicrobial active substances from Eurotiumcristatum China Food News 2022; 22(2):345-356. doi:10.16429/j.1009-7848.2022.02.037. (in Chinese).
|
| [28] |
Peng Y. The effects of Eurotium cristatum fermentation broth on immunological indicators in immunosuppressed mice Food and Mach 2021; 37(8):169-173. doi:10.13652/j.issn.1003-5788.2021.08.029. (in Chinese).
|
| [29] |
Ma YR, Wang SM, Li XZ, Wang H, He FT, Liu GP. Comparative on the functional properties of mulberry black tea fermented by Phellinus igniarius and Eurotium cristatum Chinese Edible Fungus 2022; 41(3):55-58+64. doi:10.13629/j.cnki.53-1054.2022.03.011. (in Chinese).
|
| [30] |
Yang LN, Wu KW, Xu QY, Wang RY, Qu G, Wu HT, et al. Effects of Eurotium cristatum fermentation on main quality components and flavor of Salvia plebeia tea Food and Fermn Ind 2019; 45(13):121-125. doi:10.13995/j.cnki.11-1802/ts.019965. (in Chinese).
|
| [31] |
Zou MM, Dong QH, Huang Y, Su EZ. Submerged liquid fermentation of raw dark tea by Eurotium cristatum Bioprocess Eng 2019; 17(4):409-417. doi:10.3969/j.issn.1672-3678.2019.04.012. (in Chinese).
|
| [32] |
Luo M, Yu MY, Yu LJ, Huang JA, Wang KB, Liu ZH. Effect of fermen-tation by Eurotium cristatum LJSC. 2001 on the fermentation quality of raw dark tea made from different varieties Food sci 2023; 44(14):106-115. doi:10.7506/spkx1002-6630-20221024-233. (in Chinese).
|
| [33] |
Chao J, Luo Q, Li JL, Yuan Y, Zhou MX, Yin FY, et al. Variation of char-acteristic flavor and volatile substances in Cyclocarya paliurus fer-mented with Eurotium cristatum Food and Fermn Ind 2023; 49(9):57-64+81. doi:10.13995/j.cnki.11-1802/ts.033030. (in Chinese).
|
| [34] |
Peng YQ, Liu Y, Yu LJ. Analysis of Anti-Bacterial and Quality Com-ponents Contents Varieties of Ampelopsis grossedentata-Dark Tea Compounds With Eurotium cristatum Fermentation Hubei Forestry Sci and Tech 2022; 51(3):25-31. (in Chinese).
|
| [35] |
Bo JH, Gong LJ, Ye XM, Lu ZD, Li J, Luo WM, et al. Dynamic changes of main quality components in jinhua white tea processing Mod Food Tech 2022; 38(1):306-314+20. doi:10.13982/j.mfst.1673-9078.2022.1.0471. (in Chinese).
|
| [36] |
Zheng SH, Huang D, Luo HB, Zhang LL, Gong DG, Du H, et al. Study on the quality of mulberry leaf tea fermented by Eurotium cristatum Food Sci Tech 2021; 46(3):44-48. doi:10.13684/j.cnki.spkj.2021.03.009. (in Chinese).
|
| [37] |
Yang LN, Wu KW, Lin S, Wu HT, Li DH, Du JY, et al. Orthogonal ar-ray optimization of the acanthopanax senticosus tea based on sensory evaluation J Bohai University (Nat Sci Edit) 2019; 40(1):11-18. doi:10.13831/j.cnki.issn.1673-0569.2019.01.002. (in Chinese).
|
| [38] |
Xiao Y, Li M, Liu Y, Xu S, Zhong K, Wu Y, et al. The effect of Euro-tium cristatum (MF800948) fermentation on the quality of autumn green tea. Food Chem 2021;358:129848. doi:10.1016/j.food-chem.2021.129848,PMID:33933981.
|
| [39] |
Xie J, Chen J, Mei XR, Zhu MJ, Li XL, Du J, et al. Biotransformation of Phlorizin by Eurotium cristatum to Increase the Antioxidant and Antibacterial Activity of Docynia indica Leaves. Curr Microbiol 2021; 78(4):1590-1601. doi:10.1007/s00284-021-02366-3,PMID:33686505.
|
| [40] |
Liu L, Shi J, Yuan Y, Yue T. Changes in the metabolite composition and enzyme activity of fermented tea during processing. Food Res Int 2022;158:111428. doi:10.1016/j.foodres.2022.111428,PMID:35840188.
|
| [41] |
An T, Chen M, Zu Z, Chen Q, Lu H, Yue P, et al. Untargeted and targeted metabolomics reveal changes in the chemical constituents of instant dark tea during liquid-state fermentation by Eurotium cristatum. Food Res Int 2021;148:110623. doi:10.1016/j.foodres.2021.110623,PMID:34507767.
|
| [42] |
Xiao Y, Zhong K, Bai JR, Wu YP, Gao H. Insight into effects of isolat-ed Eurotium cristatum from Pingwu Fuzhuan brick tea on the fer-mentation process and quality characteristics of Fuzhuan brick tea. J Sci Food Agric 2020; 100(9):3598-3607. doi:10.1002/jsfa.10353,PMID:32100298.
|
| [43] |
Ding L, Li XY, Liu JX, Wang YC. Study on the technology of dande-lion fermented tea Food R&D 2020; 41(15):75-79. doi:10.12161/j.issn.1005-6521.2020.15.014. (in Chinese).
|
| [44] |
Wang L. Development and quality analysis of solid-state fermented tartary buckwheat leaves tea fermented by Eurotium cristatum [Dissertation]. Chengdu: Chengdu University; 2023. doi: 10.27917/d.cnki.gcxdy.2022.000217. (in Chinese)
|
| [45] |
Zhang LH, Li ZZ, Zhao GY, Zong W. Process optimization of fermenta-tion of Eucommia ulmoides tea by Eurotium cristatum Sci Tech Food Ind 2019; 40(21):118-123. doi:10.13386/j.issn1002-0306.2019.21.019. (in Chinese).
|
| [46] |
Zou JM, Song ZR, Cai DL, Cai BY, Zhang JH, Zhang GG. Analysis on the main active components and antioxidant activity of Tieguanyin tea fermentation by Eurotium cristatum Sci Tech Food Ind 2021; 42(22):369-375. doi:10.13386/j.issn1002-0306.2021020174. (in Chinese).
|
| [47] |
Wen YJ, Peng SF, Feng J, Liu RG, Kou J. The main components and efficacy of Fu tea Food Safety Guide 2022(11):190-192. doi:10.16043/j.cnki.cfs.2022.11.037. (in Chinese).
|
| [48] |
Zheng MX, Li HJ, Chen SN, Zhao Z, Liu XQ, Li XH, et al. Effect of fer-mentation with Eurotium cristatum on aroma components of tea infusions Food Sci 2019; 40(18):223-228. doi:10.7506/spkx1002-6630-20181022-230. (in Chinese).
|
| [49] |
Zou M, Guo X, Huang Y, Cao F, Su E, Wang J. Improvement of the qual-ity of Ginkgo biloba leaves fermented by Eurotium cristatum as high value-added feed. Processes 2019; 7(9):627. doi:10.3390/pr7090627.
|
| [50] |
Xiao Y, Li M, Liu Y, Xu S, Zhong K, Wu Y, et al. The effect of Euro-tium cristatum (MF800948) fermentation on the quality of autumn green tea. Food Chem 2021;358:129848. doi:10.1016/j.food-chem.2021.129848,PMID:33933981.
|
| [51] |
Tu Z, Mei HL, Li H, Liu XQ, Emmanuel A, Zhang CL, et al. Effects of co-fermentation by Eurotium cristatum and Lactobacillus plantar-um on the quality of green tea liquid beverage Tea Sci 2018; 38(5):496-507. doi:10.13305/j.cnki.jts.2018.05.007. (in Chinese).
|
| [52] |
Xiao Y, Zhong K, Bai JR, Wu YP, Zhang JQ, Gao H. The biochemical characteristics of a novel fermented loose tea by Eurotium cristatum (MF800948) and its hypolipidemic activity in a zebrafish model. LWT 2020;117:108629. doi:10.1016/j.lwt.2019.108629.
|
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