Frontiers in Biology >

2007 , Vol. 2 >Issue 4: 403 - 407

DOI: https://doi.org/10.1007/s11515-007-0061-z

Karyotyping of Brassica oleracea L. based on rDNA and Cot-1 DNA fluorescence in situ hybridization

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  • 1.College of Life Sciences, Henan Normal University, Xinxiang 453007, China; 2.Library, Henan Polytechnic University, Jiaozuo 454000, China; 3.College of Life Sciences, Zhengzhou University, Zhengzhou 450001, China; 4.Institute of Oil Crops, Chinese Academy of Agricultural Sciences, Wuhan 430062, China;

Published date: 05 Dec 2007

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Abstract

To explore an effective and reliable karyotyping method in Brassica crop plants, Cot-1 DNA was isolated from Brassica oleracea genome, labeled as probe with Biotin-Nick Translation Mix kit, in situ hybridized to mitotic spreads, and where specific fluorescent bands showed on each chromosome pair. 25S and 5S rDNA were labeled as probes with DIG-Nick Translation Mix kit and Biotin-Nick Translation Mix kit, respectively, in situ hybridized to mitotic preparations, where 25S rDNA could be detected on two chromosome pairs and 5S rDNA on only one. Cot-1 DNA contains rDNA and chromosome sites identity between Cot-1 DNA and 25S rDNA was determined by dual-colour fluorescence in situ hybridization. All these showed that the karyotyping technique based on a combination of rDNA and Cot-1 DNA chromosome landmarks is superior to all but one. A more exact karyotype of B. oleracea has been analyzed based on a combination of rDNA sites, Cot-1 DNA fluorescent bands, chromosome lengths and arm ratios.

Cite this article

WANG Taixia, WU Chunhong, HUANG Jinyong, WEI Wenhui . Karyotyping of Brassica oleracea L. based on rDNA and Cot-1 DNA fluorescence in situ hybridization[J]. Frontiers in Biology, 2007 , 2(4) : 403 -407 . DOI: 10.1007/s11515-007-0061-z

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