In the attempt to discover new genes involved in the floral development in monocotyledonousin species, we have cloned and characterized the homologous PISTALLATA-like (PI-like) gene from Phalaenopsis hybrid cultivar named PhPI9 (Phalaenopsis PI STILLATA # 9). The cDNA of PhPI9 has a fragment of 834 bp and has 60% identity with the PISTILATA from Arabidopsis. The deduced amino acid sequence of PhPI9 had the typical PI-motif. It also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Southern analysis showed that PhPI9 was present in the Phalaenopsis orchid genome as a single copy. Furthermore, it was expressed only in the lip of the Phalaenopsis flower and no expression was detected in vegetative organs. Thus, as a B-function MADS-box gene, PhPI9 specifies floral organ identity in orchids.
GUO Bin, DAI Wei, CHEN Donghong, WEI Xing, MING Feng
. Cloning and characterization of PhPI9 involved in floral development from Phalaenopsis Orchid[J]. Frontiers in Biology, 2007
, 2(4)
: 408
-413
.
DOI: 10.1007/s11515-007-0062-y