Cloning and characterization of PhPI9 involved in floral development from Phalaenopsis Orchid

GUO Bin, DAI Wei, CHEN Donghong, WEI Xing, MING Feng

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PDF(683 KB)
Front. Biol. ›› 2007, Vol. 2 ›› Issue (4) : 408-413. DOI: 10.1007/s11515-007-0062-y

Cloning and characterization of PhPI9 involved in floral development from Phalaenopsis Orchid

  • GUO Bin, DAI Wei, CHEN Donghong, WEI Xing, MING Feng
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Abstract

In the attempt to discover new genes involved in the floral development in monocotyledonousin species, we have cloned and characterized the homologous PISTALLATA-like (PI-like) gene from Phalaenopsis hybrid cultivar named PhPI9 (Phalaenopsis PI STILLATA # 9). The cDNA of PhPI9 has a fragment of 834 bp and has 60% identity with the PISTILATA from Arabidopsis. The deduced amino acid sequence of PhPI9 had the typical PI-motif. It also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Southern analysis showed that PhPI9 was present in the Phalaenopsis orchid genome as a single copy. Furthermore, it was expressed only in the lip of the Phalaenopsis flower and no expression was detected in vegetative organs. Thus, as a B-function MADS-box gene, PhPI9 specifies floral organ identity in orchids.

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GUO Bin, DAI Wei, CHEN Donghong, WEI Xing, MING Feng. Cloning and characterization of PhPI9 involved in floral development from Phalaenopsis Orchid. Front. Biol., 2007, 2(4): 408‒413 https://doi.org/10.1007/s11515-007-0062-y
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