Frontiers in Biology >

2013 , Vol. 8 >Issue 6: 626 - 631

DOI: https://doi.org/10.1007/s11515-013-1286-7

RESEARCH ARTICLE

A functional approach toward xerogel immobilization for encapsulation biocompatibility of Rhizobium toward biosensor

  • Pooja Arora 1 ,
  • Sunita Sharma , 2 ,
  • Sib Krishna Ghoshal 3 ,
  • Neeraj Dilbaghi , 1 ,
  • Ashok Chaudhury , 1
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  • 1. Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar-125001, Haryana, India
  • 2. Light and Matter Physics Group, Raman Research Institute, Bangalore, India
  • 3. Physics Department, University Teknologi of Malaysia, Malaysia

Received date: 13 Jul 2013

Accepted date: 11 Sep 2013

Published date: 01 Dec 2013

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg
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Abstract

Sol-gel derived silica has tremendous applications as a biocompatible scaffold for the immobilization of cells. The use of xerogel as a matrix in the blueprint of biosensors is an appealing proposition due to several inimitable characteristics of xerogels, primarily because of their high porous nature, amendable pore size, and exceptionally large internal surface area. Morphological (X-Ray Diffraction and Thermogravimmetric Analysis) and optical (Fourier Transform Infrared and UV-Vis absorption) studies of the silica matrices with entrapped Rhizobial (Rz) structure of the biomaterial has been made. Temporal and concentration dependent studies were conducted for impregnated samples; it showed that the response time for the new biosensor for determining the concentration of Rz is less than 20 min. In this work, first time a novel avenue to create a generic approach for the fabrication of biosensor has been created.

Key words： biosensor; Fourier Transform Infrared spectroscopy (FTIR); Rhizobium; Thermo Gravimmetric Analysis (TGA); sol-gel; xerogel; X-Ray Diffraction (XRD)

Cite this article

Pooja Arora , Sunita Sharma , Sib Krishna Ghoshal , Neeraj Dilbaghi , Ashok Chaudhury . A functional approach toward xerogel immobilization for encapsulation biocompatibility of Rhizobium toward biosensor[J]. Frontiers in Biology, 2013 , 8(6) : 626 -631 . DOI: 10.1007/s11515-013-1286-7

Acknowledgments

One of the authors (S. Sharma) is grateful to Prof. Devendra Mohan, Guru Jambheshwar University of Science and Technology, Hisar, India and Dr. Reji Philip, Raman Research Institute, Bangalore for the useful discussions and providing the experimental facilities to carry out this work.

References

Publishing order | Descend order by publishing year | Descend order by cited within
1
Zourob M (2008). In Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems Springer, New York, pp. 109–123

2
Kishen A, John M S, Lim C S, Asundi A (2003). A fiber optic biosensor (FOBS) to monitor mutans streptococci in human saliva. Biosens Bioelectron, 18(11): 1371–1378

DOI PMID

3
Tsai H C, Doong R A, Chiang H C, Chen K T (2003). Sol-gel derived urease-based optical biosensor for the rapid determination of heavy metals. Anal Chim Acta, 48(1): 75–81

DOI

4
Barbe C J, Kong L, Finnie K S, Calleja S, Hanna J V, Drabarek E, Cassidy D T, Blackford M G (2008). Sol-gel matrices for controlled release: from macro to nano using emulsion polymerisation. J Sol-Gel Sci Technol, 46(3): 393–401

DOI

5
Desimone M F, Alvarez G S, Foglia M L, Diaz L E (2009). Development of sol-gel hybrid materials for whole cell immobilization. Recent Pat Biotechnol, 3(1): 55–60

DOI PMID

6
Gupta R, Kumar A (2008). Bioactive materials for biomedical applications using sol-gel technology. Biomed Mater, 3(3): 034005

DOI PMID

7
Shaomin L, Zhi P X, Aimin Y, Haibin S, Lihong L (2007). New biosensors made of specially designed transparent chips with nano-optical tags. Smart Mater Struct, 16(6): 2214–2221

DOI

8
MacDonald C, Morrow R, Weiss A S, Bilek M M M (2008). Covalent attachment of functional protein to polymer surfaces: a novel one-step dry process. J R Soc Interface, 5(23): 663–669

DOI PMID

9
Niki M, Solovieva N, Apperson K, Birch D J S, Voloshinovskii A (2005). Scintillators based on aromatic dye molecules doped in a sol-gel glass host. Appl Phys Lett, 86(10): 101914–101920

DOI

10
Sharma S, Mohan D, Singh N, Sharma M, Sharma A K (2008a). Spectroscopic and lasing properties in xanthene dyes encapsulated in silica and polymeric matrices. Optik (Stuttg), 121(1): 11–18

DOI

11
Sharma S, Mohan D, Ghoshal S K (2008b). Measurement of nonlinear properties and optical limiting ability of Rhodamine6G doped silica and polymeric samples. Opt Commun, 281(10): 2923–2930

DOI

12
Somasegaran P, Hoben H J (1985). Methods in legume-Rhizobium technology. NIFTAL project and MIRCEN, University of Hawaii, HI

13
Vincent J M (1970). A manual for the practical study of root-nodule bacteria. IBP Handbook15, Blackwell, Oxford, pp. 164–171

14
Rao N S S (1999). Soil Microbiology. Oxford and IBH Publishing Co. Pvt. Ltd. New Delhi, Calcutta, pp. 181–187

15
Arnon D I, Hoagland D R (1940). Crop production in artificial culture solution and in soil with reference to factors influencing yields and absorption of inorganic nutrient. Soil Sci, 50: 463–469

16
Huang Y, Siganakis G, Moharam M G, Wu S T (2004). Broadband. Optical limiter based on photo induced anisotropy of bacteriorhodopsin films. Appl Phys Lett, 5(29): 5445–5452

DOI

17
Shah N H, Hafeez F Y, Asad S, Hussain A, Malik K A (1995) Biotechnology for Sustainable Development. (Eds.): K.A. Malik A. N and Khalid A M, NIBGE, Faisalabad, Pakistan, pp. 211–217

18
Anand R C, Dogra R C (1991). Physiological and biochemiscal characteristics of fast and slow growing Rhizobium sp., from pigeon pea (Cajanus cajan). J Appl Bacteriol, 70(3): 197–204

DOI

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